Giuliano Giuseppe Stirparo

NETSseq Reveals Inflammatory and Aging Mechanisms in Distinct Cell Types Driving Cerebellar Decline in Ataxia Telangiectasia

This study uses NETSseq to analyze cerebellar tissue from A-T patients and controls, identifying cell-type-specific gene dysregulation and aging signatures linked to neurological decline.

Cell surface fluctuations regulate early embryonic lineage sorting

The paper identifies dynamic cell surface fluctuations as a key factor in early embryonic lineage sorting, showing that increased surface fluctuations in primitive endoderm precursors drive their segregation from epiblast precursors.

Human Naive Epiblast Cells Possess Unrestricted Lineage Potential

The paper shows human naive epiblast cells can generate trophectoderm and trophoblast cells, unlike mouse embryonic stem cells, and identifies key regulators of this process.

Sox2 modulation increases naive pluripotency plasticity

This study shows that low Sox2 expression in naive pluripotent stem cells enhances their developmental plasticity without impairing neuroectoderm differentiation.

Oct4 regulates embryonic pluripotency via metabolic mechanisms and Stat3 signalling.

This study identifies OCT4's role in maintaining pluripotency through metabolic regulation and Stat3 signaling in mouse embryos.

Capture of mouse and human stem cells with features of formative pluripotency

The paper identifies and characterizes formative stem cells in mice and humans, showing they have unique transcriptional and epigenetic features compared to naive and primed pluripotent cells.

Cooperative molecular networks drive a mammalian cell state transition

The paper identifies molecular networks driving the transition from naive to formative pluripotency in mammalian cells using genetic and transcriptomic analyses.

Efficient RNA-mediated reprogramming of human somatic cells to naive pluripotency facilitated by tankyrase inhibition

This paper demonstrates that tankyrase inhibition enables efficient, transgene-free reprogramming of human somatic cells to naive pluripotency, with clonal expansion and differentiation capabilities.

Single-cell transcriptome analysis of human, marmoset and mouse embryos reveals common and divergent features of preimplantation development

This study compares single-cell transcriptomes of human, marmoset, and mouse embryos to identify conserved and divergent features of preimplantation development.

Integrated analysis of single-cell embryo data yields a unified transcriptome signature for the human preimplantation epiblast

Reanalysis of three human embryo scRNA-seq datasets resolves cell-type ambiguities, defines epiblast and primitive endoderm prototypes, and identifies t2iLGö and 5i/L/A naïve stem cells as epiblast-like.