Dyann F. Wirth

Thermal proteome profiling identifies new drug targets in Plasmodium falciparum parasites

The paper identifies new drug targets in Plasmodium falciparum using thermal proteome profiling combined with molecular docking and MS-CETSA.

De novo minibinders targeting RH5 achieve nanomolar inhibition of blood-stage malaria replication

This paper designs de novo minibinders that inhibit blood-stage malaria replication with nanomolar potency by targeting the RH5-basigin interaction.

Genetic background determines allele-specific Pfkelch13-mediated artemisinin tolerance and persistence in Plasmodium falciparum

The paper shows that genetic background influences whether Pfkelch13 mutations confer short-term artemisinin tolerance or long-term persistence in Plasmodium falciparum.

PfApiAT2 is a proline transporter essential for the transmission of Plasmodium falciparum by the mosquito vector.

The paper identifies PfApiAT2 as a proline transporter essential for Plasmodium falciparum oocyst development in mosquitoes, showing that its absence leads to defective sporozoite production and oocyst degeneration.

Shared Binding Site but Divergent Resistance Profiles Uncover Novel Resistance Mechanisms in Plasmodium HSP90 Inhibitors

This study identifies divergent resistance mechanisms in Plasmodium HSP90 inhibitors despite shared binding sites, showing that enhanced binding can paradoxically correlate with resistance.

Cellular and molecular basis of PfCoronin function in artemisinin resistance in Plasmodium falciparum

This study identifies PfCoronin's role in artemisinin resistance by showing mutations impair hemoglobin uptake and modulate drug susceptibility during the ring stage of Plasmodium falciparum.

Severe malarial anaemia is associated with parasite enrichment and host alterations in the bone marrow

This study shows that severe malarial anaemia is linked to increased red blood cell production, iron metabolism, tissue injury, and parasite biomass in bone marrow, with better resolution from bone marrow analysis than peripheral blood.

Phenotypic assessment and genetic validation of Plasmodium falciparum molecular markers associated with malaria chemoprevention in Senegal

The paper evaluates the impact of drug resistance mutations on antimalarial drug resistance and fitness in Plasmodium falciparum isolates from Senegal using genetic and phenotypic assays.

The Plasmepsin-Piperaquine Paradox Persists

This study uses CRISPR/Cas9 to knockout plasmepsin II and III in Cambodian malaria parasites, showing increased sensitivity to piperaquine and linking plasmepsin gene copy numbers to PPQ resistance.

MalKinID: A Likelihood-Based Model for Identifying Malaria Parasite Genealogical Relationships Using Identity-by-Descent

MalKinID identifies malaria parasite genealogical relationships using IBD proportions and segment distributions, showing high accuracy in outcrossed importations but reduced performance in inbreeding scenarios.

Defining the Yeast Resistome through in vitro Evolution and Whole Genome Sequencing

The paper identifies genetic determinants of chemical resistance in yeast using in vitro evolution and whole genome sequencing, revealing key transcription factors involved in drug resistance.

Biomarkers to distinguish bacterial from viral pediatric clinical pneumonia in a malaria endemic setting

The paper identifies a blood protein biomarker signature to distinguish bacterial from viral/malaria pneumonia in children in a malaria-endemic setting, with high sensitivity and specificity.

hmmIBD: software to infer pairwise identity by descent between haploid genotypes

The paper introduces hmmIBD, a software tool for estimating pairwise identity by descent between haploid genomes, validated using simulated data and applied to Plasmodium falciparum samples.

De Novo Mutations Resolve Disease Transmission Pathways in Clonal Malaria

This study detects de novo mutations in Plasmodium falciparum to reconstruct disease transmission pathways and identify clade expansions not detectable with previous methods.

Mapping The Malaria Parasite Drug-Able Genome Using In Vitro Evolution And Chemogenomics

Whole genome sequencing reveals how Plasmodium falciparum evolves resistance to diverse compounds and identifies new antimalarial drug targets.