flow cytometry

HIV-1 controllers possess a unique CD8+ T-cell activation phenotype and loss of control is associated with increased expression of exhaustion markers

The paper examines CD8+ T-cell exhaustion in HIV-1 controllers and finds that loss of control correlates with increased exhaustion markers, which can be reversed by immune checkpoint blockade.

CAR Tregs mediate linked suppression and infectious tolerance in islet transplantation

A2-CAR Tregs reduce BDC2.5 T cell engraftment, proliferation, and cytokine production, protecting mice from diabetes and inducing systemic tolerance.

Brain tumor induce immunoregulatory dendritic cells in tumor draining lymph nodes that can be targeted by OX40

This study identifies immunoregulatory dendritic cells in brain tumor draining lymph nodes and shows OX40 can target them to improve immunotherapy.

Assessing a Role for Sarcospan in Immune Function

This study investigates the role of sarcospan in immune function by analyzing its expression in immune cells and assessing its impact on B cell development and effector functions in SSPN-deficient mice.

Targeting the expansion of myeloid-derived suppressor cells in liver cirrhosis

This study investigates MDSC expansion in cirrhosis patients and murine models, showing poly(I:C) reduces MDSC and fibrosis in infected fibrotic mice.

A new cellular platform for studying autophagy

This paper introduces a CRISPR-based platform to study GABARAP proteins in autophagy, revealing their distinct associations with autophagosomes.

Exhaustion profile on classical monocytes after LPS stimulation in patients with Crohn's disease.

The paper examines monocyte subsets in Crohn's disease patients, finding classical monocytes show an exhaustion profile with increased CD38 and reduced IL-1β production after LPS stimulation.

KIR3DL1 and Tox identify clonally expanded encephalitogenic neuron-specific CD8+ T cells in autoimmune encephalitis

The paper identifies clonally expanded neuron-specific CD8+ T cells in anti-Ri AIE patients using an unbiased approach and shows they express KIR3DL1 and TOX.

34-parameter full spectrum immunophenotyping panel of human regulatory and effector lymphocytes

This paper introduces a 34-marker flow cytometry panel for comprehensive immunophenotyping of regulatory and effector lymphocytes in human samples.

Microbial cancer immunotherapy reprograms hematopoietic stem cells to enhance anti-tumor immunity

This study shows that bladder BCG immunotherapy reprograms hematopoietic stem cells to enhance anti-tumor immunity in mice and humans.

Bivalent COVID-19 vaccines boost the capacity of pre-existing SARS-CoV-2-specific memory B cells to cross-recognize Omicron subvariants

Altered co-stimulatory and inhibitory receptors on monocyte subsets in patients with visceral leishmaniasis

This study examines changes in co-stimulatory and inhibitory receptors on monocyte subsets in visceral leishmaniasis patients before and after treatment.

Protective function of ex vivo expanded CD8 T cells in a mouse model of adoptive therapy for cytomegalovirus infection depends on integrin beta 1 but not CXCR3, CTLA4, or PD-1 expression

IMMUNOPHENOTYPING AND FUNCTIONAL ANALYSIS OF NK CELL SUBSETS IN Mycobacterium tuberculosis-INFECTED INDIVIDUALS

The paper analyzes NK cell subsets and function in individuals with latent TB, active TB, and healthy controls to explore their role in TB pathogenesis.

CD39 is expressed on functional effector and tissue resident memory CD8+ T cells

This study identifies CD39 expression on functional effector and tissue-resident memory CD8+ T cells, showing its role in memory differentiation and function, distinct from its role in T cell exhaustion.

Establishing a method for the cryopreservation of viable peripheral blood mononuclear cells in the International Space Station

This study develops a cryopreservation method for blood cells under ISS constraints, demonstrating viability recovery and subset analysis using flow cytometry.

Differentiation marker-negative CD4+ T cells persist after yellow fever virus vaccination and contribute to durable memory

The paper identifies TMN CD4+ T cells that persist after YFV vaccination and contribute to long-term immunological memory.

Stiffness regulates dendritic cell and macrophage subtype development and increased stiffness induces a tumor-associated macrophage phenotype in cancer co-cultures

This study shows that tissue stiffness influences dendritic cell and macrophage subtype development, with increased stiffness promoting a tumor-associated macrophage phenotype in cancer co-cultures.

Constitutive immune surveillance of nasal mucosa by three neutrophil subsets with distinct origin, phenotype, and function

This study identifies three distinct neutrophil subsets in the murine nasal mucosa with unique origins, phenotypes, and functions in immune surveillance.

Shear Stress Promotes Remodeling of Platelet Glycosylation via Upregulation of Platelet Glycosidase Activity: The Ulterior Cause of MCS-Related Thrombocytopenia?

This study shows shear stress increases platelet glycosidase activity, leading to glycosylation remodeling, platelet count decline, and microvesiculation in MCS patients.

Early cytokine-driven adaptation of survival pathways in lymphoid cells during targeted therapies

This study shows that CLL cells rapidly adapt to venetoclax by upregulating pro-survival proteins and cytokines, suggesting combination therapies targeting these pathways could improve treatment outcomes.

Reconstitution of the uterine immune milieu after transplantation

This study shows that the uterine immune system can fully reconstitute after uterus transplantation or hematopoietic stem cell transplantation, despite immunosuppressive drugs.

Multimodal and spatially resolved profiling identifies distinct patterns of T-cell infiltration in nodal B-cell lymphoma entities

Exposure to HIV alters the composition of maternal microchimeric T cells in infants

The paper examines how HIV exposure in infants affects maternal microchimeric T cells, finding reduced levels in CD8+ T cells among HIV-exposed uninfected infants.

Beta Cells Deficient for Renalase Counteract Autoimmunity by Shaping Natural Killer Cell Activity

This study shows that beta cells lacking Renalase reduce autoimmunity by altering Natural Killer cell activity and enhancing anti-inflammatory responses.

OCA-B/Pou2af1 is sufficient to promote CD4+ T cell memory and prospectively identifies memory precursors

This study shows that OCA-B expression promotes CD4+ T cell memory and identifies memory precursor cells using a reporter mouse line.

High-dimensional mass cytometry reveals stemness state heterogeneity in pancreatic ductal adenocarcinoma

The paper uses mass cytometry to analyze stemness states in PDAC, revealing molecular heterogeneity and identifying a subset with plastic phenotypes.

Streptococcal meningoencephalitis dynamically shapes developmental monocyte and macrophage trajectories in the CNS

This study shows that streptococcal meningoencephalitis alters monocyte and macrophage dynamics in the dura by shifting their origin from skull bone marrow to peripheral sources and increasing MDP-Mo-derived cells.

Introducing ARTiMiS: A low-cost flow imaging microscope for phytoplankton monitoring in engineered and natural environments

The paper introduces ARTiMiS, a low-cost flow imaging microscope for real-time phytoplankton monitoring, and demonstrates its performance in laboratory and full-scale cultivation systems.

Treponema pallidum periplasmic and membrane proteins are recognized by circulating and skin CD4+ T cells

The paper identifies Tp-specific CD4+ T cell responses in blood and skin of syphilis patients, showing persistence after treatment and highlighting potential vaccine targets.