fluorescence / live-cell microscopy

The paper uses optogenetics and cryo-ET to visualize actin cytoskeleton dynamics during lamellipodia formation, revealing distinct structural phases and actin turnover mechanisms.

This paper uses conditional GANs to colorize brightfield images of cardiac organoids, providing cell type-specific fluorescence information to enhance their characterization.

The paper introduces a method to map molecular complexes using IRIS super-resolution imaging and PC-coloring, revealing laminar complex formation in clathrin-coated structures upon EGF stimulation.

This paper analyzes the evolutionary dynamics of FoxQ2 transcription factors across metazoans, identifying three ancient paralogs and revealing conserved and novel expression patterns in chordates.

This paper designs new bimane-based fluorophores sensitive to polarity and viscosity for imaging α-synuclein in Parkinson's disease.

The paper introduces far-red chemigenetic kinase activity biosensors enabling five-color multiplexing, 4D imaging, and super-resolution via STED microscopy.

The paper uses single-molecule microscopy to show TFIIE subunits dynamically interact with preinitiation complexes, with kinetic changes influenced by TFIIH and disease mutations.

This study uses single-molecule imaging to reveal dynamic Ipl1 recruitment to kinetochores and spindles in yeast, showing changes under different conditions.

This study investigates how lenacapavir affects HIV-1 capsid structure and nuclear entry by combining simulations and live-cell imaging, showing that it causes hyperstabilization and structural defects leading to capsid failure.

This paper introduces an open-source 3D active stabilization system for fluorescence microscopy to maintain sample stability during long imaging sessions.

The paper shows that membrane-associated σ factors disrupt rRNA operon clustering in E. coli by interacting with core-RNAP and competing with σD.

This paper introduces a convolutional neural network for aberration correction in laser scanning microscopy using detector array data, achieving high-resolution imaging with minimal sample stress.

The paper introduces a method to quantify SnRK1.1 spatiotemporal localization using fluorescence confocal imaging and validates it with DCMU treatment and software comparison.

The paper introduces anionic cyanine-based plasma membrane probes for live cell and in vivo imaging, showing improved staining and visualization of neuronal structures in mice.

The paper introduces a red fluorescent Lifeact marker to study actin morphology in podocytes using intravital imaging and super-resolution microscopy.

In DT40 cells, condensins displace extrusive cohesin, bypass cohesive cohesin and stall against each other while extruding ~1-3 kb/sec, yielding irregular helical chromatids of consecutive loops.

The paper uses a SNAP25-based FRET construct to show SNARE complex assembly and disassembly dynamics in live cells, revealing that FRET ratio transients reflect complex disassembly rather than diffusion.

The paper introduces VP-CLEM-Kit, a cost-effective SR-vCLEM pipeline for visual proteomics, achieving 5-fold resolution improvement at 7-fold lower cost.

This study shows that axial asymmetry in Neisseria gonorrhoeae guides orthogonal division planes, maintaining diplococcal morphology through ParB segregation and MinCDE system function.

This study uses multi-channel smFRET to show that EF-G adopts a compact conformation with specific mutations, impacting translocation and Poly(Phe) synthesis.

This paper introduces MacqD, a deep learning model for detecting macaques in complex lab environments, achieving high F1-scores across various scenarios.

This paper introduces a method to refold fully denatured GFPs within SDS-PAGE gels using cyclodextrin and methanol, enabling in-gel fluorescence detection.

This paper introduces a Bayesian method to estimate the number of fluorescent emitters in diffraction-limited spots, showing improved accuracy over existing methods.

This paper introduces ReFLeCT, a high-speed volumetric fluorescence imaging technique that captures 4D videos of freely moving zebrafish and Drosophila larvae with high-resolution 3D reconstruction.

CellCycleNet predicts cell cycle phases from 3D nuclear-stained images, outperforming SVM models and showing strong generalization across microscopes.

This paper provides a practical guide for Single-Particle Tracking in live-cell imaging, covering sample preparation, microscope settings, and analysis software comparison.

ExSPM reveals spatiotemporal heterogeneities in lipid droplet polarity, showing dynamics and pathway-specific kinetics of lipid metabolism.

This study identifies Mpf1 as a novel factor influencing the dual targeting of tail-anchored proteins between mitochondria and peroxisomes in yeast.