protein folding & dynamics

This study characterizes the MERS-CoV replication-transcription complex and shows its structural similarity to SARS-CoV-2, with cross-species compatibility highlighting evolutionary conservation and virus-specific adaptations.

The paper introduces synthetic tools to redirect and chemically control the Arg/N-degron pathway by modifying PRT1's substrate recognition and using nanobodies to induce ubiquitination.

The paper uses deep mutational scanning to study pleiotropic effects in rhodopsin, revealing how mutations impact multiple molecular phenotypes and shape light sensitivity.

This study shows Aβ42 activates DDR2 in Alzheimer's disease models by non-canonically bypassing collagen ligands and promotes its nuclear enrichment, with structural insights into the mechanism and potential therapeutic inhibition.

This study shows the ER membrane protein complex (EMC) acts as a chaperone to facilitate the assembly of voltage-gated calcium channels during their biogenesis.

The paper profiles protein coronas on carbon nanotubes and finds that surface chemistry and dispersion forces shape corona composition and binding, with fluorescence responses partially decoupled from enrichment.

The paper investigates how ZEB85 activates TrkB by identifying a sulfotyrosine motif essential for agonist activation, while BDNF uses a different binding site.

This paper introduces Hi-APEX, a H2O2-independent APEX2 variant for in vivo spatial multi-omics, showing improved performance in capturing redox-sensitive processes and enabling in vivo applications.

This study examines how mutations in the N-terminal domain of SARS-CoV-2 Spike affect antigenic properties and immune escape, finding that structural flexibility and loss of heme-metabolite interaction reduce antibody neutralization.

This study validates engineered hyper-stable human serum albumin variants for their structural integrity and functional adaptability in biotechnological applications.

This paper introduces a series of BzAM terpolymers with varying hydrophobicity to solubilize GPCRs in nanodiscs, showing that solubilization efficiency and functional integrity are preserved.

This study shows that the LMD-3 mutation in C. elegans causes reproductive failure by disrupting lysosomal function and proteostasis, which can be reversed with vitamin B12 supplementation.

The paper uses molecular dynamics simulations to study how D-Retro-Inverso peptides modulate TTR fibril stability and identifies DRI-K6V as a potential disruptor of TTR fibrils.

This study identifies kinetic differences in RAS-BRAF engagement between KRAS and NRAS, showing how oncogenic mutations and inhibitor sensitivity are influenced by these dynamics.

HTFAST enables high-throughput, quantitative affinity measurement of cell-free expressed binders using FRET, validated with nanobodies and SARS-CoV-2 sdAbs.

This study shows that Grb2 phosphorylation at Y160 inhibits EGFR condensate formation, thereby reducing Ras activation, and reveals how kinase inhibition can promote Ras signaling under certain conditions.

This study uses molecular dynamics simulations to investigate how extracellular acidification affects the structure and function of HCN2 channels by disrupting hydrogen bond networks involving residue E212.

This paper introduces a magnetic bead-based sample preparation method for top-down proteomics to improve sensitivity and reduce sample loss during protein aggregation capture.

This paper introduces a method to measure RNA structure and protein binding in human cells, showing how RNA folding and sequence determine protein occupancy and function.

This study uses HS-AFM to show yeast TOM complexes predominantly form trimers that dynamically dissociate into dimers and monomers, revealing structural and dynamic features of the TOM complex.

This study shows KhpA and KhpB in Campylobacter jejuni depend on each other for RNA recognition, with KhpA using a KH domain and KhpB an atypical R3H domain.

The paper introduces FlexENN, a graph neural network that predicts binding energies for both folded and intrinsically disordered protein complexes, showing improved performance over rigid-body methods.

The paper predicts the structure of a 89-kDa invasion-tip protein from Holospora obtusa and suggests a pH-dependent conformational change may aid in escaping the host digestive vacuole.

This study identifies a cryptic divalent metal binding pocket that regulates Cas9's metal-dependent activity through conformational activation and catalytic efficiency.

This paper determines two X-ray crystal structures of a thermophilic lipase, showing that Pro367 ring puckering is associated with calcium binding and conformational selection.

This paper introduces HOClSense dyes, a chlorination-based indicator for subcellular HOCl detection, and demonstrates their use in identifying STING pathway-induced HOCl and abnormal HOCl production in Niemann-Pick diseases.

This study inhibits prolyl hydroxylase to enhance collagen type I production in mouse cells under normoxia, achieving a 29-fold increase.