Sperm and Testicular Function

This study uses a novel in vivo protein-degradation approach to show NFYA is essential for spermatogenesis by regulating stage-specific transcriptional programs through chromatin interactions.

This study identifies a haploid-specific genome quality control program in spermatids that eliminates damaged nuclei, revealing a non-canonical role for H2Av in maintaining genome integrity during spermatogenesis.

This study shows that ectopic SOX17 in Sertoli cells promotes Sertoli valve formation and partially rescues infertility in Sox17-null mice.

The paper uses single-cell RNA sequencing to study D. pseudoobscura testes, identifying transcriptional differences between eusperm and parasperm lineages during spermatogenesis.

This study identifies the mouse homolog of KK-LC-1, shows it is expressed in testis and sperm, and finds species-specific differences in its role in sperm motility between humans and mice.

Deep proteome and phosphoproteome of Id4-eGFP-sorted mouse spermatogonial stem and progenitor cells reveal 8,464 proteins, poor RNA-protein concordance, and phosphorylation-driven kinase candidates tested functionally.

The paper shows that properly purified cauda sperm lack somatic contamination and that pretreatment disrupts chromatin structure, validating untreated sperm profiles.

The paper measures nucleosome occupancy in mouse and human sperm using single-molecule footprinting and finds species-specific differences in residual nucleosome retention.

The paper uses phosphoproteomic analysis to identify kinases involved in human sperm capacitation, finding PLK1 inhibition affects motility and acrosome reaction.

The paper tracks clonal dynamics of spermatogonial stem cells in zebrafish over time and finds non-neutral drift patterns inconsistent with random chance.

This study shows that ACTRT2 is essential for acrosome stability in mouse sperm, with its loss causing subfertility and structural defects during spermiogenesis.

This study shows that high levels of misfolded protein aggregates in bovine sperm impair preimplantation embryo development, affecting blastocyst formation and increasing developmental arrest.

This paper develops E-InfertilityTest, an explainable AI model to classify infertile versus fertile sperm using transcriptomic data and provides gene-level explanations for predictions.

This study identifies SPE-40/FAM187 as a conserved sperm protein essential for fertilization in C. elegans, showing that its mutation causes sub-fertility without affecting sperm production or motility.

This study shows that cholesterol-based extenders can cryopreserve beef and bison semen without equilibration, reducing processing time and maintaining sperm quality.

This study identifies SPARK as a conserved multi-protein complex essential for vertebrate fertilization, linking gamete recognition to membrane fusion.

This study identifies FIMP as a binding partner of TMEM95, determines their complex structure, and shows that disrupting this interaction inhibits sperm-egg fusion.

The paper introduces hSPICER, a functional assay to assess sperm fertilizing potential by measuring cell-cell fusion with hJUNO-expressing cells, showing correlation with in vitro fertilization outcomes.

The paper identifies a population of male germ cells in mice that exhibit non-apoptotic cell death characteristics during G0 arrest, suggesting selection based on cellular health for spermatogonial stem cell precursors.

This study identifies MCT1 as a metabolic checkpoint regulator in spermatogenesis, showing its role in meiotic progression through epigenetic mechanisms.

This study identifies transcriptional states and regulators of spermatogonial stem cell differentiation in marmoset testes across developmental stages.

The paper shows SMG6 and PIWIL1 work together in the chromatoid body to regulate mRNAs via piRNA and NMD pathways, with SMG6's endonuclease activity aiding piRNA-guided degradation.

The paper defines metabolic programs during spermatogenesis by analyzing transcriptomic, proteomic, and metabolic changes across meiotic stages.

This study shows that THC exposure to postejaculatory sperm reduces motility, damages DNA, impairs acrosome integrity, alters the methylome, and decreases embryo development potential.

This study finds that cancer survivors have a higher prevalence of loss of Y chromosome in their spermatozoa compared to controls, suggesting it as a potential biomarker for genomic instability and disease risk.

This study evaluates a novel sperm capacitation technology, HyperBull, in bovine IVF and finds it increases hatched blastocyst rates compared to conventional methods.

The paper defines a 3D chromatin compartment blueprint that programs histone-to-protamine exchange during spermiogenesis using ATAC-seq, CUT&Tag, and Hi-C.

This study maps chromatin accessibility and protamine incorporation during spermiogenesis, revealing compartment-specific dynamics and their role in genome stability.

This study shows that acute paternal immune activation alters sperm small RNAs, which reprogram embryonic gene expression and enhance offspring survival from viral infection.