DNA Repair Mechanisms

This study examines the distinct roles of RNase HII and HIII in removing RNA:DNA hybrids and their impact on mutagenesis and DNA replication.

This study shows that catalytically inactive PARP1 causes embryonic lethality and genome instability in a dominant-negative manner, revealing its severe impact on normal tissues.

The paper identifies epistasis between mutator alleles in mice, showing that combined mutator alleles at two loci increase C>A mutations compared to single mutator alleles.

This study investigates how changing protein-DNA interactions enable ORC to switch DNA binding sites during replication origin licensing, revealing a stepwise mechanism involving DNA unbending, sliding, and reduced ORC stability.

The paper investigates how ORC phosphorylation inhibits helicase loading by preventing Mcm2-7 ring closure in budding yeast.

The paper investigates the genetic factors influencing error-prone NHEJ repair of chromosomal DSBs with 5' overhangs in yeast, identifying roles for Pol4, TLS polymerases, and Exo1/Sgs1.

The paper develops Indel-Seq to study insertion mechanisms at DNA double-strand breaks, finding insertions arise from transcription and require DNA-PK and ssDNA overhangs.

The paper identifies two distinct pathways of genome rearrangement induced by multi-invasion during homologous recombination and their impact on genome stability.

This study shows that long-range DNA end resection by Exo1 and Sgs1-Dna2 supports homologous recombination through checkpoint activation, not by generating extensive homology.

This study identifies TLK1-mediated phosphorylation of RAD54 at specific sites that regulate homologous recombination repair, with distinct effects on repair efficiency and cell survival.

The paper shows that endogenous oxidative stress drives mutagenesis in bacteria through transcription-coupled repair, contributing to antibiotic resistance evolution.

This study investigates the role of the SAP domain in Ku-DNA interaction phases, showing it facilitates initial binding but not sliding, and finds that reduced loading efficiency does not impair NHEJ or telomere maintenance in Arabidopsis.

The paper investigates how the intracellular level of Rad51 in S. cerevisiae is regulated through proteolysis involving SUMO and ubiquitin modifications.

This study reports cryo-EM structures of nucleosomes with deoxyinosine and their complexes with DNA glycosylase AAG, revealing how AAG accesses damaged sites on nucleosomes through structural dynamics.

This study shows ATR kinase prevents replication resource exhaustion in early S phase, supporting normal cell proliferation by regulating origin firing.

This study identifies SIRT1's role in regulating DNA damage signaling via interaction with the PP4 phosphatase complex, showing SIRT1 inhibits PP4 to modulate key DDR markers.

This study shows that progerin expression in a mouse model shifts DNA double-strand break repair from precise ligation to error-prone non-homologous end joining, potentially contributing to genomic instability and aging.

The paper identifies PRDX1 as essential for DNA damage repair by scavenging nuclear ROS and regulating aspartate synthesis during the DNA damage response.

This study identifies ATM phosphorylation of DNA-PKcs at T4102 as critical for stabilizing NHEJ machinery and promoting DSB repair.

The paper identifies HEIP1 as a conserved protein involved in meiotic crossover formation, showing its role in both early and late stages of the class I crossover pathway in Arabidopsis and its presence in mammals.

This study identifies Ixr1's role in DNA replication stress response through its interaction with Mrc1 and DNA binding domains, showing sensitivity to HU and gene expression changes.

This study identifies a PP4-dependent mechanism for Hop1 and Red1 assembly onto meiotic chromatin in budding yeast, independent of DSB formation.

The paper identifies actin nucleators as essential for protecting replication forks by modulating RPA activity and preventing ssDNA degradation during replicative stress.

This study investigates neurodegeneration in Xeroderma pigmentosum using stem cell-derived neurons, identifying endoplasmic reticulum stress and UPS dysfunction as key factors and developing an early detection assay.

The paper identifies Rad53-dependent protein localization changes during replication stress, revealing Mec1-independent activation of Rad53 in yeast.

The paper identifies Rpa1/Ssb1 mutants in fission yeast with partial checkpoint signaling defects, providing tools to study RPA's multiple functions.

This study identifies the FIGNL1-FIRRM complex as essential for completing meiotic recombination in mice by regulating RAD51 and DMC1 loading, independent of DNA damage.

This study characterizes the NSE6 subunit of the moss Physcomitrium patens SMC5/6 complex, identifying a conserved motif and functional roles in complex dynamics and DNA repair.

This study identifies higher-order coordination of DNA repair proteins in single cells by analyzing their genome-wide localization using DamID and ChIC sequencing.

This study identifies UVSSA's role in transcription-coupled repair of DNA interstrand crosslinks and links its expression to chemotherapy resistance in cancer cells.