Advanced biosensing and bioanalysis techniques

Allosteric Ligand-Aptamer Complexes Orchestrate Supramolecular or Transient Catalytic, Transcription and Fibrinogenesis Processes

This paper demonstrates allosteric ligand/aptamer complexes that regulate catalytic, transcription, and fibrinogenesis processes through supramolecular assembly and transient stabilization.

Catch-and-Display Immunoassay for Digital Biomarker Detection

This paper introduces a cost-effective digital immunoassay called CAD-IA for detecting low-abundance biomarkers, demonstrating its sensitivity and linearity using the TBI biomarker S100B.

Nanowire-based biosensor for short DNA using fluorescent silver nanoclusters

This paper introduces a nanowire-based biosensor using fluorescent silver nanoclusters for sensitive, label-free detection of short DNA without amplification, achieving single-molecule sensitivity for HIV-1 DNA.

Emergence of a new material through ligation of DNA nanostar hydrogels

This paper ligates DNA nanostar hydrogels to improve their mechanical properties and nuclease resistance, enabling their use as programmable biomaterials.

LASSO: versatile and selective biomolecule pulldown with combinatorial DNA-crosslinked polymers

LASSO is a sequence-selective biomolecule isolation platform that achieves high efficiency, low off-target effects, and cost-effectiveness for diverse targets.

DNA nanodevice for analysis of force-activated protein extension and interactions

The paper develops a DNA nanodevice to apply controlled forces to proteins, demonstrating its use in studying mechanosensitive protein interactions and identifying novel tension-dependent binders.

gFET-based aptasensors technology allows sensitive and specific quantification of the ESKAPE pathogens

This paper develops a gFET-based aptasensor platform for rapid, sensitive, and specific detection of ESKAPE pathogens with high analytical performance.

Hairpin-Functionalized Gold Nanoparticles as an Adaptable Platform for Detecting MicroRNA Signatures

This paper presents a gold nanoparticle-based sensor for detecting microRNA signatures with high sensitivity and versatility, adaptable for point-of-care applications.

Bioimaging with fluorescent nucleic-acid aptamers for the specific detection and quantification of Pseudomonas aeruginosa alone and in heterogeneous bacterial populations

This study develops a fluorescent aptamer-based bioimaging method to detect Pseudomonas aeruginosa with high specificity and quantifies its performance using automated software.

Self-assembled cell-scale containers made from DNA origami membranes

This paper introduces DNA origami membranes that self-assemble into cell-scale containers, enabling programmable compartmentalization for bottom-up biology and soft robotics.

Multi-stem-loop constrained library enables accelerated SELEX for aptamers with superior kinetics and affinity

This paper develops a multi-stem-loop constrained library to accelerate SELEX for aptamers, resulting in candidates with superior binding kinetics and affinity in fewer rounds.

Information Theory-Guided Detection of Biomarkers Using Programmable Aptamer Arrays

The paper introduces a nucleic acid device called CDA for point-of-care diagnosis using endogenous biomarkers, demonstrating high classification accuracy and efficient diagnostic strategies.

Unconventional DNA architecture in a dopamine-bound aptamer complex

This study determines the structure of a dopamine-binding DNA aptamer using NMR, revealing an unconventional DNA fold distinct from typical DNA structures and providing insights into its specificity and potential applications in biosensor design.

DNA Motors Powered by Exonuclease III for Autonomous Rolling Motion and Biosensing Applications

This paper develops an RNA-free DNA motor powered by Exonuclease III, demonstrating autonomous rolling motion and biosensing capabilities through aptamer-functionalized components.

Improving Electrochemical Aptasensor Sensitivity for Bacillus cereus Spore Detection in Food Safety Applications

This paper develops an electrochemical aptasensor for rapid detection of Bacillus cereus spores in food, achieving low limits of detection and high specificity.

Proximity-based super-resolution imaging enabled by DNA base-stacking interactions

This paper introduces Stack-pPAINT, a super-resolution imaging technique using DNA base-stacking interactions to detect proximal target molecules, demonstrating its application in visualizing microtubular structures.

SELEX-HTCFQ Platform: Developing DNA Enhancers of ADAR1 to Suppress ZBP1-Dependent Immunopathology

The paper develops a SELEX-HTCFQ platform to identify ADAR1-specific enhancers that suppress ZBP1-dependent immunopathology by selectively modulating ADAR1 activity.

Single-Cell Super-Resolution Quantification of Oxidative DNA Damage via Aptamer-Assisted DNA-PAINT

This paper develops a super-resolution imaging method to quantify oxidative DNA damage at the single-cell level using DNA aptamers and DNA-PAINT, showing improved specificity and resolution over traditional methods.

A facile and reproducible method for the purification of peptide- and protein-functionalized DNA nanostructures

This paper introduces a fast and efficient purification method for peptide- and protein-functionalized DNA nanostructures with high yield and purity.

Inter-Instrument Quantification of Fluorescence Read-out Signal Using DNA Origami Calibrator Beads

This study uses DNA origami calibrator beads to calibrate and compare the sensitivity of five flow cytometers for detecting dimly fluorescent nano-sized particles.

Compact RNA sensors for increasingly complex functions of multiple inputs

The paper designs compact RNA sensors for complex functions of multiple inputs using crowdsourced designs and an algorithm, achieving applications like tuberculosis diagnosis.

Ultrasensitive graphene FET aptasensor for direct attomolar detection of glutamate in human clinical samples

This paper develops an ultrasensitive graphene FET aptasensor for detecting glutamate in clinical samples, showing high sensitivity and clinical relevance in Alzheimer's Disease.

Controlled reassociation of multistranded, polycrossover DNA molecules into double helices

This paper demonstrates temperature and sequence-controlled reassociation of polycrossover DNA nanostructures into duplexes using anti-PX structures and formamide.

Captamer: A Novel Quantitative Protein Detecting Method Depending on Aptamer-activated Molecular Switches and RPA Signal Amplification

Captamer is a new protein quantification method using aptamer-based molecular switches and RPA amplification, achieving high sensitivity and rapid detection of proteins like SARS-CoV-2 nucleocapsid and Tau441.

MEMO: A Micro Memo Sensor Detecting microRNA-RISC using an Accurate Cell-Free Expression Platform

The paper introduces a cell-free biosensor for detecting microRNA-RISC interactions with high sensitivity and specificity.

DNA branch migration across intact membranes powers an inside-out GPCR analog for lysis-free detection of intracellular RNA with on-membrane amplification

This paper demonstrates DNA branch migration across lipid membranes to detect intracellular RNA without lysing cells, using a synthetic GPCR-like system called HALOS.

Customizable silicification of DNA origami nanostructures

This study introduces two customizable silicification methods for DNA origami nanostructures to enhance their functionality and stability in various environments.

Spring-loaded DNA origami arrays as energy-supplied hardware for modular nanorobots

This paper introduces reconfigurable DNA origami arrays as a network of two-state systems to enable multi-stimuli, multi-output nanorobots with controlled logic and timing.

Vesicle-templated self-assembly of programmable freestanding multi-μm DNA shells

This paper introduces a method to create freestanding DNA shells that mimic lipid membranes, demonstrating their ability to retain geometric features from templates and form tunable compartments.

Reversible Sandwich-Based Particle Nanoswitch for Continuous Protein Monitoring at Picomolar Concentrations with Automated Calibration

This paper introduces a reversible particle nanoswitch for continuous protein monitoring at picomolar concentrations with automated calibration, achieving accurate long-term measurements.