Advanced biosensing and bioanalysis techniques

Resolving multivalent antibody binding states with defined spatial antigen-patterning

This paper introduces PANMAP, a new assay that measures antibody avidity profiles by controlling antigen spacing, revealing how spatial constraints affect binding equilibria.

Lifetime-based multiplexed detection of viral RNA using fluorogenic aptamers

This paper introduces a fluorescence-lifetime-based multiplexed detection strategy using fluorogenic aptamers to distinguish viral RNA targets with minimal cross-reactivity and spectral overlap.

Coding Agents as a Mechanism for Formalizing and Transferring Domain Knowledge in DNA Origami Design

This paper introduces a coding agent that formalizes and transfers domain knowledge in DNA origami design through text-based instructions, enabling autonomous design and simulation.

Mechanical properties of DNA double-crossover motifs

The paper investigates the mechanical properties of DNA double-crossover motifs using molecular dynamics simulations and finds anisotropic bending rigidity and twist stiffness.

Alpha-tocopherol conjugated DNA tetrahedron with enhanced cellular uptake and selective cytotoxicity for cancer therapeutics

This paper develops a DNA tetrahedron conjugated with alpha-tocopherol to enhance cellular uptake and selectively kill cancer cells through ROS-induced apoptosis.

Beyond Metabolites: A Wearable Differential Biointerface Integrating Antibody and Aptamer Probes for the Real-Time Tracking of Proteins In Vivo

The paper introduces a wearable device for real-time protein monitoring using antibody and aptamer probes, demonstrating its effectiveness in tracking cardiac troponin I in vivo.

Label-free toehold mediated strand displacement on 3D printed hybrid paper-polymer platform for protein sensing

This paper develops a 3D printed DNA logic gate for detecting PDGF BB with pico molar sensitivity and specificity.

A rapid, sensitive, and quantitative high plex biomarker digital detection platform enabled by Hypercoding

This paper introduces Hypercoding, a high-plex biomarker detection platform that enables sensitive and quantitative analysis of multi-omic targets with high accuracy and resolution.

In-Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing

This paper introduces a method to isolate ssDNA from dsDNA sources for DNA origami assembly, demonstrating its effectiveness across various dsDNA types and lengths.

Mechanistic insights into the color transformation of a non-FRET substrate for RNase activity detection

This paper identifies the mechanism of color change in DNA/AgNC substrates upon nucleolytic cleavage and demonstrates their application in sensitive virus detection.

Clamp the LAMP: a photoelectrochemical platform for KRAS mutation detection via wild-type blocking

This paper introduces a photoelectrochemical platform for KRAS mutation detection that uses molecular suppression of wild-type alleles to enhance sensitivity and specificity.

Visualizing and Quantifying microRNA Induced DNA Origami Separation at the Nanoscale

This paper presents a method using DNA origami disassembly driven by miRNA to detect small RNA, showing robust detection in RNAse-containing environments.

Magnetic DNA Origami Nanorotors

This paper demonstrates magnetic DNA origami nanorotors that can be controlled and rotated using magnetic fields, achieving torque values of 10-100 pN nm.

Modular Scaffold Crystals for Programmable Installation and Structural Observation of DNA-Binding Proteins

This paper develops modular DNA scaffold crystals for programmable installation and structural observation of DNA-binding proteins, enabling high-throughput structure determination and functional applications.

Intracellular Expression of a Fluorogenic DNA Aptamer Using Retron Eco2

This study demonstrates the use of the bacterial retron system Eco2 to express a DNA aptamer intracellularly in E. coli, showing comparable activity to chemically synthesized controls.

Tunable DNA Origami Nanosensors for Detection of Multiscale Spatial Ion Concentration Gradients

This paper introduces a DNA origami-based sensor (NanoDyn) capable of detecting Na+ ion gradients across multiple spatial scales, demonstrating tunable sensitivity and resolution.

Evolutionary selection of DNA nanostructures for cellular uptake

This paper develops an evolutionary selection method to identify DNA nanostructures with cell-specific uptake by screening large libraries in mammalian cells.

AptaBLE: A Deep Learning Platform for Aptamer Generation and Analysis

AptaBLE is a deep learning framework that predicts aptamer-protein binding and generates novel aptamers with high specificity and low Kd values.

High-Performance, Computer-Controlled Bipedal DNA Motor

This paper describes a high-fidelity, computer-controlled DNA motor that moves bidirectionally with high efficiency and precision, outperforming previous designs.

Programmable RNA detection generates DNA barcodes for multiplexed phage-host interaction screening

This paper introduces Detectrons, RNA-to-DNA transduction biosensors for phage-host interaction screening, enabling multiplexed detection and quantitative susceptibility profiling.

Visualizing single base RNA mutations in living cells through DNA nanostructure mediated amplification

The paper introduces a DNA nanostructure-based platform for single-base RNA mutation detection in living cells, demonstrating its ability to distinguish insulin mRNAs and detect KRASG12D mutations with high specificity.

Selective and robust dopamine detection is enabled by aptamer-SWCNT optical sensors in physiological media

This study develops dopamine-selective aptamer-SWCNT sensors that show improved selectivity and sensitivity in complex biological media.

Modular DNA nanosensors enable state-aware multiplexed single-molecule mass photometry in complex media

This paper introduces modular DNA nanosensors that enable label-free, multiplexed single-molecule mass photometry to resolve protein biochemical states in complex media without washing or immobilisation.

Uncovering Design and Assembly Rules for mRNA-DNA Origami

This study identifies design and assembly rules for mRNA-DNA hybrid origami to enable high-yield formation of stable nanostructures.

Multiplexed cellular and tissue imaging via plasmonic heating activated signal exchange of DNA probes

This paper introduces PHASER, a method for rapid, multiplexed fluorescence imaging using plasmonic heating to activate DNA thermal probes, achieving 5 targets in a single channel.

Predicting DNA origami stability in physiological media by machine learning

This paper uses machine learning to predict DNA origami stability in physiological conditions based on physicochemical parameters and DLS measurements.

Engineered retrons generate genome-independent protein-binding DNA for cellular control

The paper demonstrates using engineered retrons to generate programmable DNA for sequence-specific protein-binding, enabling dynamic control of gene networks and feedback circuits in living cells.

Complex cooperativity in DNA origami revealed via design dependent defectivity

The paper explores cooperativity in DNA origami by analyzing design variations and their impact on assembly yield, identifying metrics that correlate with defectivity and yield.

Bacteriophage-Mimetic DNA Origami Needle for Targeted Membrane Penetration and Cytosolic Cargo Delivery

This paper describes a DNA origami needle that targets cancer cells and delivers cargo into the cytosol by mimicking bacteriophage mechanisms.

Aptamer-based DNA nanoswitches for multiplexed protein detection

The paper develops aptamer-based DNA nanoswitches for detecting proteins, demonstrating dual detection of thrombin and VEGF.