Lipid Membrane Structure and Behavior

Two temperature-dependent membrane fluidity regimes in Gram-positive bacteria

The paper measures membrane fluidity in Gram-positive bacteria across temperatures and finds fluidity is maintained at low temperatures but not at higher temperatures.

Anionic bacterial sphingolipids increase membrane stiffness

This study identifies anionic bacterial sphingolipids, specifically ceramide phosphoglycerate, and shows they increase membrane zeta potential and stiffness.

Membrane curvature enhances oxidation within lipid bilayers in a composition-dependent manner

This study shows that membrane curvature and lipid composition together influence oxidation rates in lipid bilayers, with curvature increasing oxidation and cholesterol modulating this effect.

Fusion peptides of enveloped viruses actively mediate membrane fusion in model cell membranes

The paper investigates how fusion peptides from enveloped viruses mediate membrane fusion in model cell membranes using microfluidic devices and fluorescence microscopy.

ATP-driven membrane binding and polymerization of bacterial actin MreB promotes local membrane fluidization

This study investigates how ATP and ADP binding affects MreB polymerization and membrane interaction in Geobacillus stearothermophilus, revealing distinct binding affinities and membrane fluidizing effects.

Quantitative biophysical analysis of human septin hexamer and octamer self-assembly on model membranes

This study quantitatively compares the membrane binding and self-assembly of human septin hexamers and octamers using biophysical methods, revealing distinct structural properties and functional implications.

MEMBRANE PORES ACT AS SELF-RESEALING LIPID SCRAMBLASES

The paper shows that lipid asymmetry drives pore formation in membranes, which function as self-resealing scramblases to restore balance.

A single vesicle fluorescence microscopy platform to quantify phospholipid scrambling

The paper introduces a single vesicle fluorescence microscopy platform to quantify phospholipid scrambling by VDAC1 dimers and opsin, revealing kinetic heterogeneity in scrambling rates.

Membrane Lipid Composition and Asymmetry Act as a Switch for Cell-Penetrating Peptide Translocation versus Leakage

This paper shows that membrane lipid composition and asymmetry determine whether a CPP translocates or causes leakage, using simulations and experiments.

DNA Double-decker Ring Scaffolded Nanodisc for Self-assembly of Membrane Protein into Lipid Bilayer

This study introduces a DNA-based nanodisc scaffold for membrane protein assembly, demonstrating its compatibility with various lipids and detergents.

Two-Dimensional Phase Separation of DNA Nanomotifs Anchored to Lipid Bilayers

This paper studies DNA nanomotifs anchored to lipid bilayers, showing they undergo two-dimensional phase separation based on sticky end interactions, independent of lipid phase separation.

Bigger Isn't Always Better: Comparing System Size, Hydration, and Software for Lipid Membrane Analysis

This study compares membrane structural properties across different system sizes and hydration levels, and evaluates four analysis software tools for their performance and consistency.

Membrane structural properties in Staphylococcus aureus are tuned by the carotenoid 4,4'-diaponeurosporenoic acid

This study investigates how 4,4'-DNPA, a carotenoid in S. aureus, affects membrane biophysical properties, showing it increases membrane order and stability compared to STX.

High-Throughput Characterization of Trends in Transmembrane Helix Partitioning into Membrane Domains

This study uses high-throughput simulations to analyze transmembrane helix partitioning in lipid membranes, finding most peptides prefer the disordered phase and some localize at the interface.

Peptide-DNA Conjugates for Formation of Lipid Nanodiscs

This paper introduces DNA-templated peptide-DNA conjugates that form defined lipid nanodiscs with controlled structure and peptide arrangement.

Quantifying interleaflet coupling of phase behavior and observing anti-registered phases in asymmetric lipid bilayers

This study quantifies interleaflet coupling in asymmetric lipid bilayers and observes anti-registered phases in 14:1-PC systems.

Bacterial lipids traverse the hydrophobic groove of TamB

This study shows that TamB transfers lipids through a hydrophobic groove connecting inner and outer membranes in Gram-negative bacteria, with lipid release dependent on TamA binding.

A Dynamic NMR Lineshape Simulation Framework for Lipid Diffusion and Membrane Thinning in Bicelles and Nanodiscs

The paper presents a dynamic NMR lineshape simulation framework for lipid diffusion and membrane thinning in bicelles and nanodiscs, enabling accurate interpretation of anisotropic NMR spectra.

Slow diffusion and signal amplification on membranes regulated by phospholipase D

This study shows phospholipase D (PLD) reduces lipid and protein diffusion in membranes by producing phosphatidic acid, which affects phosphoinositide signaling and membrane protrusion.

Quantifying 3D Live-Cell Membrane Dynamics Using Dynamic Metal-Induced Energy Transfer Spectroscopy (dynaMIET)

This paper introduces dynaMIET, a method to measure 3D membrane dynamics in live cells, and shows it can track lateral diffusion and vertical fluctuations simultaneously.

Amino Acid Insertion Energetics in a POPC Bilayer from Unbiased Molecular Dynamics

The paper quantifies amino acid side-chain insertion energetics in a POPC bilayer using unbiased molecular dynamics simulations and derives potentials of mean force to characterize bilayer partitioning.

Photoreceptor outer segment disk rim curvature relies on a tetraspanin interaction web

This study investigates the structural role of PRPH2 and Rom1 in photoreceptor outer segment disk curvature using BN-PAGE and MD simulations, revealing their interaction web and curvature mechanisms.

Deciphering Membrane Pore Formation Mechanisms of Plasmodium falciparum Perforin-Like Protein 1 (PfPLP1)

This study models the structure and simulates the membrane binding and pore formation mechanism of PfPLP1, revealing how it interacts with host cell membranes and forms pores.

Exploring the effects of Golgi Reassembly and Stacking Proteins in lipid membranes

This study investigates the interaction of myristoylated GRASP65 and GRASP55 with lipid membranes using reconstitution and various analytical techniques, revealing their impact on membrane dynamics.

DNA-Lipid Nanodiscs with a Polyethylene Glycol Interface

This paper introduces DNA-Lipid Nanodiscs (DLNs) with a PEG interface to study membrane proteins and lipid bilayers, showing effective shielding of hydrophobic mismatch through PEG-lipid interactions.

Coarse-Grained Simulations of Mycobacterial Outer Membranes Reveal Fluidity-Dependent PDIM Redistribution Across Different Lipid Environments

This study develops and validates coarse-grained models of mycobacterial outer membranes to investigate lipid organization and PDIM behavior under different lipid environments.

Mechanisms controlling the plasma membrane targeting and the nanodomain organization of the plant SPFH protein HIR2

This study identifies S-acylation and oligomerization as key factors controlling HIR2 plasma membrane targeting and nanodomain organization in Arabidopsis.

Unexpected softening of giant unilamellar vesicles by budding yeast septin filaments: a curvature dependent mechanism

The paper investigates how budding yeast septin filaments affect the mechanical properties of giant unilamellar vesicles (GUVs), finding that large GUVs become more deformable while small ones become less deformable due to septin-induced membrane reshaping.

Differential effects of lysophospholipid headgroups, acyl chain length and saturation on vacuole acidification, Ca2+ transport, and fusion

The paper examines how lysophospholipid headgroups and acyl chain properties affect yeast vacuole fusion, Ca2+ transport, and acidification.

Membrane dynamics-mediated heat retention dictates economical intracellular energy flow

This study experimentally demonstrates intracellular heat retention mediated by membrane dynamics, showing heat generated by metabolism is partially retained and used for efficient energy flow.