Microfluidic and Capillary Electrophoresis Applications

3D Hydrodynamic Flow Lithography

This paper introduces a 3D hydrodynamic flow lithography method to rapidly generate complex microstructures with tailored functionalities.

Selective Laser Etching technology for reconfigurable microfluidic and electrochemistry-on-chip

The paper introduces Selective Laser Etching (SLE) for creating 3D microfluidic and electrochemical devices, demonstrating its use in fabricating functional sliding elements for reconfigurable chips.

Dual-Mode Microfluidic Immunostaining (Dumi) Device for Diagnostic Biomarkers Detection and Tumor Microenvironment Evaluation

The paper introduces a microfluidic device that reduces tissue usage for immunostaining and TME analysis in rare tumors, achieving >90% reduction in section usage and enabling comprehensive biomarker detection.

Deterministic DNA barcoding using vacuum-driven loading of free oligonucleotides to microwell arrays

This paper introduces a deterministic DNA barcoding method using vacuum-driven microfluidics to load oligonucleotides into microwells, achieving uniform barcoding with reduced reagent use and cross-contamination.

Large field of view fluorescence imaging of microfluidic devices with a tandem-lens macroscope

This paper introduces a macroscope for wide-field fluorescence imaging in microfluidic devices, achieving high sensitivity and temporal resolution over a large area.

Magnetically Responsive Nanocultures for Direct Microbial Assessment in Soil Environments

The paper introduces magnetic nanocultures for isolating and growing environmental microbes under near-native conditions, demonstrating their feasibility for discovering uncultured species.

Single-cell Organelle Extraction with Cellular Indexing

The paper introduces VacTrap, a microfluidic device that isolates and spatially indexes single nuclei from mammalian cells with high efficiency, enabling single-cell multiomics applications.

Paired Analyses of Nuclear Protein Targets and Genomic DNA by Single-Cell Western Blot and Single-Cell PCR

The paper introduces SplitBlot, a microfluidic single-cell assay that pairs genomic DNA analysis with nucleo-cytoplasmic protein measurements from the same cell.

Integrating microfluidic automation into thermoplastic devices for analysis of small volumes of blood

This paper develops a thermoplastic microfluidic device for automated blood analysis, demonstrating plasma extraction and simultaneous detection of glucose, lactate, and H2O2 with performance comparable to standard methods.

Spatiotemporal control of a multilayered co-axial flow in a 3D printed microchannel with cascaded nozzles

The paper develops a 3D-printed microfluidic device with cascaded nozzles to control multilayered co-axial flows, achieving a minimal delay time and reduced backflow index.

Development of an on-chip fluorescence anisotropy immunoassay for human C-peptide secretion reveals a general roadmap for tracer optimization

The paper develops an on-chip fluorescence anisotropy immunoassay for human C-peptide by optimizing antibody-tracer pairs to achieve high sensitivity and fast kinetics for microfluidic measurement.

Microvalve-based devices for flow-free gradient generators

This paper introduces microvalve-based devices for flow-free gradient generation, enabling precise control and automation of microfluidic experiments.

A microfluidic platform for extraction and analysis of bacterial genomic DNA

This paper introduces a microfluidic platform for extracting and analyzing bacterial genomic DNA without fragmentation, demonstrating its use on B. subtilis cells.

Teach your microscope how to print: Low-cost and rapid-iteration microfabrication for biology

This paper introduces a low-cost microfabrication method using 3D printing and repurposed microscopes for biological applications, achieving micrometer precision without clean room infrastructure.

A single microfluidic device for multi-omics analysis sample preparation

The paper develops a microfluidic device for simultaneous proteomic and genomic analysis of microbial samples, showing it can process low-abundant samples with reduced bias.

The dynamics of capillary flow in an open-channel system featuring trigger valves

This paper demonstrates the implementation of trigger valves in open-channel microfluidic systems and develops a model to describe their flow dynamics.

Electromigration of Charged Analytes Through Immiscible Fluids in Multiphasic Electrophoresis

This study investigates how an interleaving oil layer affects the electrotransfer of charged analytes into a hydrogel, showing increased peak skew and dispersion due to oil viscosity and phase mobility mismatch.

5-axis CNC micro-milling machine for three-dimensional microfluidics

This paper introduces a 5-axis CNC micro-milling machine for prototyping 3D microfluidic channels with high resolution, geometric complexity, and material versatility.

Microfluidic interfaces for chronic bidirectional access to the brain.

This paper develops microfluidic interfaces for chronic fluid access to the brain in mice, enabling precise injection and long-term sampling of CSF and biomarkers.

Direct encapsulation of biomolecules in semi-permeable microcapsules produced with double-emulsions

This paper describes a method to directly encapsulate biomolecules in semi-permeable microcapsules using double-emulsions, enabling applications in diagnostics and therapeutics.

On demand nanoliter sampling probe for collection of brain fluid

This paper introduces a new microfabricated neural probe that enables precise, transient-free sampling of brain extracellular fluid biomarkers in vivo and in vitro.

Microfluidic-based imaging of complete C. elegans larval development

This paper introduces a microfluidic imaging method for long-term, stable, and parallel live-imaging of C. elegans larval development, enabling observation of previously inaccessible processes.

A plug and play microfluidic platform for standardized sensitive low-input Chromatin Immunoprecipitation

This paper introduces a microfluidic platform for standardized, sensitive ChIP-Seq on low-input samples, enabling high-throughput and reproducible epigenetic profiling.

Sequencing Metrics of Human Genomes Extracted from Single Cancer Cells Individually Isolated in a Valveless Microfluidic Device

This paper describes a valveless microfluidic device for isolating and sequencing single cancer cells, achieving high-quality genome coverage with minimal contamination.

Microfluidic Transfection for High-Throughput Mammalian Protein Expression

This paper describes a microfluidic transfection device that enables high-throughput mammalian protein expression with controlled culturing and real-time imaging.

Openspritzer: an open hardware pressure ejection system for reliably delivering picolitre volumes.

Openspritzer is an open hardware system for delivering picolitre volumes with performance comparable to commercial systems, demonstrated in neurobiology applications.