Ion channel regulation and function

Epileptic encephalopathy-related Kv2.1 mutants impair channel clustering and membrane distribution but not neuronal excitability

This study examines how mutations in Kv2.1, a potassium channel linked to epileptic encephalopathy, affect channel clustering and neuronal excitability, finding impaired clustering but no change in excitability.

Ionic conductances driving tonic firing in Purkinje neurons of larval zebrafish

The paper identifies L-type calcium and SK-type potassium currents as essential for tonic firing in larval zebrafish Purkinje neurons, proposing a conserved mechanism for sustained high-frequency firing.

Nckx30c, a Drosophila K+-dependent Na+/Ca2+ exchanger, regulates temperature-sensitive convulsions and age-related neurodegeneration

This study identifies Nckx30c as a Drosophila Na+/Ca2+ exchanger that regulates temperature-sensitive convulsions and age-related neurodegeneration through its role in Ca2+ homeostasis.

A bi-directional binding site linking the α2δ-1 subunit to the intrinsic speed control process in VSD I of voltage-gated calcium channels

This study identifies a bi-directional binding site between the α2δ-1 subunit and the voltage-sensing domain I of calcium channels, revealing how it modulates activation kinetics.

Neuromodulation of zebrafish primary motoneuron firing is shaped by developmental changes in the M-current

The paper investigates how neuromodulators like serotonin and acetylcholine modulate the M-current in zebrafish primary motoneurons during development, showing age-dependent effects on firing properties.

Early-stage determinants of T1-S1 conformations in Kv1.3 channels

This study identifies molecular determinants of early-stage folding in Kv1.3 channels, revealing dynamic interfaces and domain interactions that influence tetramerization and conformation.

Evolution towards higher unitary conductance in mammals makes BK channels more efficient and precise

This study shows that mammalian BK channels have higher unitary conductance, leading to more efficient calcium activation and stronger potassium currents compared to invertebrate channels.

HCN channel currents underlie distinct neurophysiology of mediodorsal thalamus subnuclei

This study identifies differences in HCN channel activity across mediodorsal thalamus subnuclei, linking these differences to distinct intrinsic oscillatory properties and cellular resonance.

An ion channel omnimodel for standardized biophysical neuron modelling

The paper introduces a standardized ion channel model based on Hodgkin-Huxley formulation, demonstrating its ability to fit most voltage-gated models and enable parameter comparison and inference.

Intrinsic mechanisms contributing to the biophysical signature of mouse gamma motoneurons

This study identifies intrinsic biophysical properties of gamma motoneurons in mice, revealing mechanisms underlying their low recruitment threshold and high firing rates.

Secondary structure transitions and dual PIP2 binding define cardiac KCNQ1-KCNE1 channel gating

This study determines the structure of KCNQ1+KCNE1 in closed and open states, revealing structural transitions and dual PIP2 binding that regulate channel gating and IKs function.

Natural xanthones as α-Mangostin induce vasorelaxation involving key gating residues in the S6 domain of BK channels

The paper identifies Mangostin as a BK channel activator that induces vasorelaxation by modulating key S6 domain residues.

Electrophysiological classification of CACNA1G gene variants associated with neurodevelopmental and neurological disorders

This study uses automated and manual patch-clamp techniques to analyze Cav3.1 channel variants, identifying gain- and loss-of-function properties linked to neurological disorders.

Lipids modulate open probability of RyR1 under cryo-EM conditions

This study shows that lipid concentration affects RyR1 open probability under cryo-EM conditions, with higher concentrations increasing open probability but lipid nanodiscs keeping RyR1 closed.

Tracing the evolutionary origins of human voltage-gated K+ channels: where they come from and what we lost along the way

The paper traces the evolutionary origins of human voltage-gated K+ channels, identifying which were present in common ancestors and which were lost, and functionally characterizes the missing channels from other vertebrates.

Atomistic Simulation of Voltage Activation of a Truncated BK Channel

The paper uses atomistic simulations to study voltage activation of BK channels, revealing unique VSD movement and coupling mechanisms distinct from canonical Kv channels.

Shaker it OFF: Biophysical Characterization of an Inactivating Potassium Conductance Mediating Object Segmentation in a Collision-Detecting Neuron

The paper characterizes a Shaker potassium channel in a grasshopper neuron involved in visual object segmentation through electrophysiology and computational modeling.

Ca2+-calmodulin regulates Kv7.1 channel gating by allosterically interfering with its inactivation path

The paper identifies the inactivation paths of Kv7.1 channels and shows how Ca2+-calmodulin prevents inactivation through allosteric interference.

Atomistic Mechanism of Cation Permeation and Pore Voltage Sensitivity in Cyclic Nucleotide-Gated CNGA1 Ion Channel

The paper uses molecular dynamics simulations to reveal the atomistic mechanism of cation permeation and voltage sensitivity in the CNGA1 ion channel.

YnaI exemplifies the diversity of structural gating mechanisms in mechanosensitive channels of small conductance

The paper investigates the structural gating mechanisms of YnaI, showing it opens via radial relocation of sensor paddles and pore shortening, contrasting with MscS's tilt-based mechanism.

Sevoflurane inhibition of the developmentally expressed neuronal sodium channel Nav1.3

This study investigates how sevoflurane affects Nav1.3 sodium channels in developing neurons, showing voltage-dependent inhibition and altered inactivation properties.

IonBench: a benchmark of optimisation strategies for mathematical models of ion channel currents

IonBench evaluates optimization strategies for ion channel models, identifying an efficient approach that reduces computation time and improves reliability.

Distinct electrophysiological signatures reveal neuronal heterogeneity in the mouse fasciola cinereum

This study identifies distinct electrophysiological properties of neurons in the mouse fasciola cinereum, revealing their unique intrinsic excitability and synaptic characteristics compared to neighboring hippocampal regions.

Effects of Natural Lithium and Lithium Isotopes on Voltage Gated Sodium Channel Activity in SH-SY5Y and IPSC Derived Cortical Neurons

This study investigates how lithium isotopes affect sodium channel activity in SH-SY5Y and iPSC-derived cortical neurons, finding no significant differences in SH-SY5Y cells but small differences in iPSC-derived neurons.

Atomistic Mechanism of Non-Canonical Voltage Gating in TREK K2P Channels

This paper reveals the atomistic mechanism of voltage gating in TREK K2P channels through molecular dynamics simulations and electrophysiology, showing how SF conformational flexibility and water influx regulate ion permeation.

Sensitivity analysis of voltage-gated ion channel models.

This study analyzes how model topology and stimulation protocols affect parameter accessibility in voltage-gated ion channel models using Sobol sensitivity analysis.

Structural determinants for GqPCR-mediated inhibition of TASK K2P K+ channels and their dysfunction in disease

This study identifies the molecular mechanisms of GqPCR-mediated inhibition of TASK-1 and TASK-3 K2P channels and links their dysfunction to disease.

Structural and Mechanistic Insights into Symmetry Conversion in Plant GORK K+ Channel Regulation

The paper solves the cryo-EM structure of Arabidopsis GORK, revealing symmetry reduction driven by ANK dimerization, which regulates K+ channel activity in guard cells.

Voltage Sensor Conformations Induced by LQTS-associated Mutations in hERG Potassium Channels

The paper identifies intermediate voltage-sensor conformations in hERG channels caused by LQTS-associated mutations using FLIM, tmFRET, and MD simulations.

Investigating the Functional Role of the DI-DII Linker in Nav1.5 Channel Function

This study investigates the functional role of the I-II linker in Nav1.5 channels, finding that a point mutation significantly affects channel gating while deletions have minimal impact.