Fungal and yeast genetics research

Tor1 dysfunction promotes phenotypic diversity in the asexual fungus Candida albicans

The paper shows that TOR pathway dysfunction increases phenotypic switching in C. albicans, suggesting this process is regulated.

Long-read genomes reveal pangenomic variation underlying yeast phenotypic diversity

The paper uses long-read sequencing to identify pangenomic genes contributing to yeast phenotypic variation by resolving QTLs that are missed by short-read methods.

Overexpression profiling reveals cellular requirements in context of genetic backgrounds and environments

The paper identifies genes whose overexpression is adaptive under stress in budding yeast, revealing cellular requirements influenced by genetic background and environment.

Boosting N-terminally anchored yeast surface display via structural insights into S. cerevisiae Pir proteins

The paper uses structural insights into yeast Pir proteins to engineer more efficient N-terminally anchored surface display constructs in S. cerevisiae.

Repeat-induced point mutation and gene conversion coinciding with heterochromatin shape the genome of a plant pathogenic fungus

The paper analyzes meiosis-associated genetic changes in Zymoseptoria tritici using whole-genome sequencing, finding high recombination rates, gene conversions in heterochromatin, and repeat-induced point mutations as a major source of de novo mutations.

Identification of Aspergillus niger aquaporins involved in hydrogen peroxide signaling

The paper identifies AQPF, an aquaporin in Aspergillus niger, as facilitating hydrogen peroxide transport across the cell membrane, supporting its role as a signaling molecule in glucose oxidase induction.

Proteome dynamics during transition from exponential to stationary phase under aerobic and anaerobic conditions in yeast

This study quantifies proteome dynamics in yeast during the transition from exponential to stationary phase under aerobic and anaerobic conditions, revealing significant differences in proteome changes between the two environments.

Coding Sequence Insertions in Fungal Genomes are Intrinsically Disordered and can Impart Functionally-Important Properties on the Host Protein

The paper identifies and characterizes clade-defining insertions in fungal proteomes, showing they are intrinsically disordered and can confer functional properties on host proteins.

The ethanol tolerance in Saccharomyces cerevisiae under a phenomics perspective

The paper investigates ethanol tolerance in Saccharomyces cerevisiae by analyzing lncRNAs and metabolic pathways in high and low tolerance strains.

Conservation and Expansion of Transcriptional Factor Repertoire in the Fusarium oxysporum Species Complex

This study examines transcription factor repertoires in the Fusarium oxysporum species complex, finding expanded families linked to host-specific adaptation and pathogenicity.

Rgs1 is a regulator of effector gene expression during plant infection by the rice blast fungus Magnaporthe oryzae

The paper identifies Rgs1 as a regulator of effector gene expression in the rice blast fungus M. oryzae, showing it controls at least 60 effector genes before plant infection.

Intergenic regions of Saccharomycotina yeasts are enriched in potential to form transmembrane domains

The paper investigates TM-forming potential in intergenic regions of Saccharomycotina yeasts and finds it is pervasive but variable across species.

Biocontainment strategies for in vivo applications of Saccharomyces boulardii

This paper develops a biocontainment strategy for Saccharomyces boulardii by knocking out THI6 and BTS1, resulting in auxotrophy and cold sensitivity, which limits its growth outside the host.

The GUN mutants: new weapons to unravel ascospore germination regulation in the model fungus Podospora anserina

The paper identifies GUN1, a gene involved in ascospore germination in Podospora anserina, and shows its conservation with Pth2 in Magnaporthe oryzae.

A high-quality reference genome for the fission yeast Schizosaccharomyces osmophilus

This paper presents a high-quality reference genome for S. osmophilus, a newly identified fission yeast species, and uses it to conduct comparative genomic analyses across fission yeast species.

Recognition of Galactose by a Scaffold Protein Recruits a Transcriptional Activator for the GAL Regulon Induction in Candida albicans

The paper identifies Rep1 as a galactose sensor in Candida albicans that recruits Cga1 to activate GAL gene transcription, revealing a novel regulatory mechanism in fungal metabolism.

The Adr1 transcription factor directs regulation of the ergosterol pathway and azole resistance in C. albicans

This study identifies Adr1 as a transcription factor regulating ergosterol pathway and azole resistance in C. albicans, with functional interactions with Upc2.

High quality de novo genome assembly of non-conventional yeast Kazachstania bulderi a new potential low pH production host for biorefineries.

The paper presents a high-quality genome assembly of Kazachstania bulderi, a non-conventional yeast, using long-read sequencing to support biotechnological applications.

Mining novel cis-regulatory elements from the emergent host Rhodosporidium toruloides using transcriptomic data

The paper uses transcriptomic data from Rhodosporidium toruloides to identify novel cis-regulatory elements by predicting transcription factor binding motifs under stress and substrate conditions.

Lessons from the meiotic recombination landscape of the ZMM deficient budding yeast Lachancea waltii

The paper analyzes meiotic recombination in Lachancea waltii, identifying differences in crossover distribution, LOH events, and recombination hotspots compared to related species.

Improving Therapeutic Protein Secretion in the Probiotic Yeast Saccharomyces boulardii using a Multifactorial Engineering Approach

This paper improves therapeutic protein secretion in Saccharomyces boulardii using genetic modifications and proteomics, achieving >10-fold higher secretion of a neutralizing peptide.

PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae

The paper investigates how PKA signaling coordinates growth and metabolism in yeast during anaerobic xylose fermentation by analyzing transcriptomic, lipidomic, and phosphoproteomic data.

Tup1 is Critical for Transcriptional Repression in Quiescence in S. cerevisiae

This study shows that Tup1 is essential for transcriptional repression in quiescent S. cerevisiae cells during glucose starvation.

Dynamic metabolome profiling uncovers potential TOR signaling genes

The paper uses dynamic metabolomics to identify potential TOR signaling genes in yeast by analyzing metabolite responses to rapamycin treatment.

OPENPichia: building a free-to-operate Komagataella phaffii protein expression toolkit

The paper develops an open-source yeast chassis, OPENPichia, for synthetic biology by reverse engineering and characterizing industrial Pichia strains.

Comprehensive re-analysis of hairpin RNAs in fungi reveals ancestral links

The paper re-analyzes mi/milRNA hairpin RNAs in 40 fungal species, identifies high-quality loci, and provides a centralized resource for future research.

Developing a temperature-inducible transcriptional rheostat in Neurospora crassa

The paper characterizes the temperature responsiveness of hsp promoters in Neurospora crassa, identifying hsp30 as a highly tunable promoter for synthetic biology applications.

Strain dependent differences in coordination of yeast signaling networks

This study compares two yeast strains to show how genetic variation affects signaling network coordination between the HOG and mating pathways.

An adaptive interaction between cell type and metabolism drives ploidy evolution in a wild yeast

The paper shows that ploidy reduction in yeast populations evolved for maltose utilization is driven by an interaction between cell type and metabolism, with haploid-like transcriptional programs conferring fitness advantages.

Modeling single-cell phenotypes links yeast stress acclimation to transcriptional repression and pre-stress cellular states

The paper models single-cell phenotypes in yeast to show that transcriptional repression during stress improves post-stress growth recovery.