Fungal and yeast genetics research

Comparative proteome analysis of different Saccharomyces cerevisiae strains during growth on sucrose and glucose

The paper compares proteomes of three Saccharomyces cerevisiae strains grown on sucrose and glucose, finding Mnp1 is consistently higher on sucrose and opposite biological function patterns between strains.

Protein kinase A gene knockout in Coprinopsis cinerea by CRISPR/Cas9 RNP complex system

This study uses CRISPR/Cas9 RNP to knockout the CcPka1 gene in Coprinopsis cinerea, revealing its role in nutrient signaling and fruiting body formation.

Architectural Groups of a Subtelomeric Gene Family Evolve Along Distinct Paths in Candida albicans

The paper examines the evolution of the TLO gene family in Candida albicans, revealing distinct evolutionary paths among structural groups and identifying functional motifs.

Gene mapping methodology powered by induced genome rearrangements

This study compares TAQing and conventional mutagenesis to identify genes responsible for flocculation in yeast, finding TAQing narrows down candidate loci more effectively.

Reconstruct a eukaryotic chromosome arm by de novo design and synthesis

This paper designs and synthesizes a functional neochromosome in yeast that replaces a native chromosome arm, demonstrating genome plasticity and synthetic chromosome viability.

Reduced Cdc14 phosphatase activity impairs septation, hyphal differentiation and pathogenesis and causes echinocandin hypersensitivity in Candida albicans

This paper shows that reducing Cdc14 phosphatase activity in Candida albicans impairs cell wall integrity, septation, hyphal differentiation, and virulence, making it a potential antifungal drug target.

Integrative functional analysis uncovers metabolic differences between Candida species

The paper identifies metabolic differences between Candida species using genomic, metabolomic, and gene expression data, highlighting unique pathways in the AGAu cluster.

Genome-wide base editor screen identifies regulators of protein abundance in yeast

The paper uses a CRISPR base editor screen in yeast to identify regulators of protein abundance, revealing both specific and broad regulatory relationships.

Top-down, knowledge-based genetic reduction of yeast central carbon metabolism

The paper reduces genetic complexity in yeast central carbon metabolism by deleting 35 genes, resulting in minimal phenotypic changes under various conditions.

Complex genetics cause and constrain fungal persistence in different parts of the mammalian body

The paper identifies genetic loci influencing fungal persistence in different mammalian organs using a yeast model, showing complex interactions between general and organ-specific alleles.

The protein phosphatases MoPtc1 and MoPtc2 are induced during pathogen-host interactions and play synergistic roles in regulating MAPK pathways in Magnaporthe oryzae

This study identifies MoPtc1 and MoPtc2 as type 2C phosphatases that synergistically regulate MAPK pathways in Magnaporthe oryzae during pathogen-host interactions.

A moonlighting function of a chitin polysaccharide monooxygenase, CWR-1, in allorecognition in Neurospora crassa

The paper identifies CWR-1 as a chitin polysaccharide monooxygenase involved in allorecognition in Neurospora crassa, showing its PMO domain mediates cell fusion blockage through protein-protein interactions with CWR-2.

Constitutive activation of TORC1 signaling attenuates virulence in the cross-kingdom fungal pathogen Fusarium oxysporum

This study shows that constitutive activation of TORC1 signaling in Fusarium oxysporum reduces virulence by impairing invasive hyphal growth and pathogenicity in both plant and animal hosts.

Laboratory evolution and polyploid SCRaMbLE reveal genomic plasticity to synthetic chromosome defects and rearrangements

The paper debugs a synthetic yeast chromosome and uses laboratory evolution to identify genomic rearrangements that suppress growth defects caused by synthetic modifications.

Synthetic yeast chromosome XI design enables extrachromosomal circular DNA formation on demand

The paper constructs a synthetic yeast chromosome XI and demonstrates how non-coding DNA modifications affect cell fitness, enabling on-demand extrachromosomal circular DNA formation.

Ddp1 cooperates with Ppx1 to counter a stress response initiated by non-vacuolar polyphosphate

The paper investigates how Ddp1 and Ppx1 counter polyphosphate toxicity in yeast by analyzing stress responses and enzyme functions.

Large excess capacity of glycolytic enzymes in Saccharomyces cerevisiae under glucose-limited conditions

The paper shows that Saccharomyces cerevisiae maintains excess glycolytic enzyme capacity under glucose-limited conditions as an adaptation to environmental nutrient fluctuations.

Using canavanine resistance to measure mutation rates in Schizosaccharomyces pombe

The paper measures mutation rates in S. pombe using canavanine resistance and finds that spontaneous mutants predominantly have an R175C mutation in the any1 gene, which confers resistance through altered arginine transport and cell morphology.

Bursts of genomic instability potentiate phenotypic and genomic diversification in Saccharomyces cerevisiae

The paper shows that stochastic genomic instability in yeast leads to phenotypic and genomic diversification through transient bursts of structural variations.

High sorbic acid resistance of Penicillium roqueforti is mediated by the SORBUS gene cluster

The paper identifies the SORBUS gene cluster as the mediator of high sorbic acid resistance in Penicillium roqueforti through genome-wide association, SNP analysis, and genetic engineering.

CeGAL: revisiting a widespread fungal-specific TF family using an in silico error-aware approach to identify missing zinc cluster domains

The paper identifies that most MHD-only fungal TFs lack a DNA binding domain due to annotation errors, redefining the CeGAL family as a new TF family with Zn2C6-MHD domains.

Genomic expression responses in sensu stricto Saccharomyces yeast to DNA damage induced by methyl methanesulfonate

The paper examines transcriptome responses to MMS-induced DNA damage in four Saccharomyces species, revealing species-specific gene expression changes and functional genomic insights.

Transcription factor lineages in plant-pathogenic fungi, connecting diversity with fungal virulence

This study analyzes transcription factor diversity across plant-pathogenic fungi to understand its relationship with virulence.

LncRNAs of Saccharomyces cerevisiae dodge the cell cycle arrest imposed by the ethanol stress

The paper investigates how two lncRNAs in Saccharomyces cerevisiae influence cell cycle arrest during ethanol stress, showing they bypass checkpoints in different tolerant strains.

Molecular cloning and recombinant protein expression of 9-Lipoxygenase gene from Solanum tuberosum in Pichia pastoris yeast cells

This study clones and expresses a 9-lipoxygenase gene from Solanum tuberosum in Pichia pastoris to produce recombinant protein for biodegradable fungicide development.

Unlocking the functional potential of polyploid yeasts

The paper introduces a method to improve industrial yeast strains through recombination during mitotic growth, identifying clones with enhanced biotechnological traits.

Regulated use of alternative Transcription Start Sites controls the production of cytosolic or mitochondrial forms of branched-chain aminotransferase in Kluyveromyces marxianus

The paper investigates how alternative transcription start sites regulate the production of cytosolic and mitochondrial branched-chain aminotransferase proteoforms in Kluyveromyces marxianus, showing condition-specific regulation by transcription factors.

Comprehensive analysis of meiosis-derived cDNA libraries reveals gene isoforms and mitochondrial proteins important for competitive fitness

The paper constructs and analyzes meiotic cDNA libraries in yeast to identify gene isoforms and mitochondrial proteins affecting competitive fitness and respiration.

Aneuploidy influences the gene expression profiles in Saccharomyces pastorianus group I and II strains during fermentation.

This study shows that gene copy number correlates with expression levels of orthologous genes in Saccharomyces pastorianus strains during fermentation, influencing fermentation properties through differential contributions of S. cerevisiae and S. eubayanus alleles.

Full-length Genome of a Ogataea polymorpha strain CBS4732 ura3Δ reveals large duplicated segments in subtelomeric regions

The paper reports the full-length genome of O. polymorpha HU-11/CBS4732, revealing large duplicated segments in subtelomeric regions and structural variations between CBS4732 and NCYC495.