Fungal and yeast genetics research

PKA-regulated carbohydrate production protects cells by altering cytoplasmic biophysical properties

This study shows PKA regulates cytoplasmic density by controlling glycogen and trehalose, which affect diffusion and confer stress resistance.

Contrasting evolutionary trajectories of nitrate assimilation across Brettanomyces bruxellensis lineages

The paper investigates nitrate assimilation in B. bruxellensis lineages using genomic and phenotypic data, finding it is widespread but unevenly distributed and influenced by gene copy number and functionality.

Genetic determinants that permit growth without the core septin Cdc12 in Cryptococcus neoformans

The paper identifies genetic factors that allow Cryptococcus neoformans to grow without the core septin Cdc12, revealing compensatory mechanisms for septin-independent cell integrity.

A fungal hemophore relay mediates heme transfer via transient protein interactions

This study investigates heme transfer in Candida albicans using fluorescent hemophore fusions, revealing distinct kinetic mechanisms for heme acquisition and transfer between hemophores.

Capture of the SmSTRIPAK proxiome identifies the greenbeard proteins SmDOC1/2 as regulators of the MAK2 pathway to control sexual development in Sordaria macrospora

The paper identifies SmDOC1/2 as regulators of the MAK2 pathway in Sordaria macrospora, showing non-redundant roles in sexual development through proximity mapping and genetic analysis.

A Glucan Synthase-Remodeler Module Organizes Branched Glucan Assembly in the Fungal Cell Wall

This study identifies Ghs2 as a binding partner of Bgs3 in fission yeast, showing they co-localize and work together to synthesize and branch β-1,3-glucan in the fungal cell wall.

Four-species Aspergillus pan-GWAS reveals rare genome expansion in pathogenicity and contraction in domestication

This study performs pan-GWAS across four Aspergillus species to show that pathogenicity involves rare genome expansion while industrial strains show rare genome contraction.

A haploid wild yeast resource for exploring the natural ecology of Saccharomyces cerevisiae

This paper establishes a haploid yeast strain collection to study ecological and reproductive divergence in wild S. cerevisiae, finding that structural genomic variation and metabolic gene divergence drive these traits.

Post-translational modification fidelity of recombinant human lactopontin expressed in Kluyveromyces lactis

This study shows that recombinant human lactopontin produced in Kluyveromyces lactis has structural and functional similarities to native human lactopontin, with consistent phosphorylation and glycosylation patterns across production scales.

Expansion and optimization of the auxin-inducible degron 2 (AID2) system in Candida pathogens

The paper expands and optimizes the AID2 system for use in Candida species, including clinical isolates and C. auris, enhancing its utility for protein function studies.

Transcriptional responses of acute glucose deprivation reveal a role for Snf12 and Spt20 in metabolic adaptation during stress

This study investigates the transcriptional responses of yeast to glucose deprivation, identifying roles for Snf12 and Spt20 in metabolic adaptation and stress signaling.

Species Context Reverses PPIP5K Control of Fungal Morphogenesis and Actin Organization

The paper shows that the PPIP5K enzyme Asp1 produces opposite effects on fungal morphology and actin organization in different species, depending on the host cellular environment.

Repurposing native non-homologous end joining for multicopy random integration in Wickerhamomyces ciferrii

This paper repurposes the native non-homologous end joining (NHEJ) bias in Wickerhamomyces ciferrii to create a multicopy genome integration platform for metabolic engineering.

Experimental design and analysis of sulfide-induced invasive growth in wine yeast

This study identifies conditions for observing sulfide-induced invasive growth in wine yeast and shows genetic and environmental factors influence this behavior.

A CandiChrome toolkit for multicolor labeling of Candida cells

The paper develops CandiChrome, a multicolor labeling toolkit for Candida albicans, enabling strain labeling, mixed-population imaging, and competition assays with up to 21 distinct populations resolution.

Glucose repression of HXK1 is glucose flux-dependent via non-canonical regulation of Mig1

This study shows that glucose repression of HXK1 depends on glucose flux rather than concentration, mediated by the Snf1/AMPK pathway at low glucose levels and an unknown mechanism at high levels.

Distinguishing Polymer Turnover from De Novo Biosynthesis Reveals Dynamic Cell Wall Remodeling During Aspergillus fumigatus Conidial Germination

The paper distinguishes polymer turnover from de novo biosynthesis in Aspergillus fumigatus during conidial germination using isotope-edited solid-state NMR, revealing dynamic cell wall remodeling.

The hybrid yeast Saccharomyces pastorianus TUM 34/70 repurposes the pseudohyphal growth signaling network to establish a flocculation phenotype

The paper identifies Ste20p and Ypi1p as key proteins involved in flocculation in S. pastorianus by repurposing the pseudohyphal growth signaling network.

cis- and trans-regulatory factors contributing to divergent activity of the TDH3 promoter in Saccharomyces yeast

The paper identifies cis-regulatory changes in the TDH3 promoter that increase expression in S. cerevisiae by altering transcription factor interactions, while preserving expression dynamics.

Humanization of N-glycan-dependent protein quality control system in Kluyveromyces marxianus promotes glycoprotein secretion

The paper enhances glycoprotein secretion in yeast by engineering a human-like protein quality control system, resulting in up to 12-fold increased production of therapeutic glycoproteins.

The Candida Genome Database: New Interface and New Tools

The paper describes updates to the Candida Genome Database, including a new interface and tools for improved data access and analysis.

A recent domestication event in Penicillium biforme, independent of the emblematic cheese mold P. camemberti

The paper identifies a new Penicillium biforme lineage, cheesy, selected for food fermentation, with genetic and phenotypic differences from P. camemberti and traits advantageous for cheesemaking.

A Multiplex Droplet Digital PCR Assay for Chromosome Copy Number Determination in Candida albicans

The paper develops and validates a 16-plex droplet digital PCR assay for accurate chromosome copy number quantification in C. albicans, showing strong correlation with whole-genome sequencing.

Synthetic Combinatorial Minimisation of Cell Cycle Control in Yeast

This study minimizes the yeast cell cycle gene set using synthetic genomics, identifying minimal gene combinations that maintain viability and robustness across conditions.

The Ancient Origin and Dynamic Diversification of the Fungal Poly(ADP)-ribose Polymerase Protein Family

This study analyzes the evolution of fungal PARP proteins across 534 species, identifying dynamic diversification and lineage-specific gains and losses.

Elevated substitution rates and increased purifying selection associated with thermophily in Chaetomiaceae fungi

The paper investigates genomic adaptations in thermophilic Chaetomiaceae fungi, finding higher substitution rates and stronger purifying selection compared to mesophiles.

Chromosome-scale CRISPR screening reveals secretory pathway genes as drivers of aneuploidy-mediated antifungal tolerance

The paper uses CRISPR screening to identify secretory pathway genes that drive antifungal tolerance in Candida albicans through aneuploidy.

Komagataella phaffii encodes two functional Pho4 transcription factors

The paper identifies two functional Pho4 transcription factors in Komagataella phaffii and shows they have overlapping but distinct roles in stress response.

Solid-state NMR Reveals Mobility-Based Organisation of the Schizosaccharomyces pombe Cell Wall

This study uses solid-state NMR to show how glucan synthases affect the molecular organisation and dynamics of the S. pombe cell wall through mobility-based reorganisation.

Paralogs of the Candida albicans TLO gene family form interconnected functional networks with incomplete redundancy

The paper investigates paralog redundancy in the Candida albicans TLO gene family, finding that while most paralogs show redundancy, some exhibit non-redundant functions and form interconnected networks.