Advanced Fluorescence Microscopy Techniques

The paper introduces new voltage sensors that enable simultaneous cellular-resolution activity imaging from multiple neuron-types in awake animals.

The paper develops improved genetically-encoded cAMP sensors, cAMPFIREs, for high-sensitivity in vivo imaging of cAMP dynamics in neurons and Drosophila.

The paper describes a mounting protocol for live imaging of C. elegans larvae and adults using light sheet fluorescence microscopy.

This study compares GCaMP-X and GCaMP for long-term Ca2+ imaging in neurons, finding GCaMP-X provides better long-term expression and less cytotoxicity.

EZ Clear provides a simple, rapid, and robust method for clearing whole adult mouse organs, preserving fluorescence and enabling downstream analysis.

FT-GO improves fluorescent histochemical analysis by enhancing signal detection while reducing background noise in mouse and marmoset brains.

This paper develops a new genetically encoded fluorescent cAMP indicator, G-Flamp1, which shows high sensitivity, brightness, and fast response kinetics for in vivo cAMP imaging.

Deep-SMOLM improves 3D orientation and 2D position measurement of overlapping single molecules with high precision and speed.

This paper demonstrates the use of SPAD sensors to image neural activity at kilohertz frame rates, resolving subthreshold and spiking activity with shot noise limited signals.

This paper develops a TIS window technique for long-term high-resolution cortical imaging in awake mice without skull removal.

This study shows that striatal fiber photometry signals mainly reflect non-somatic calcium changes, not somatic ones.

The paper introduces mBeRFP, a red fluorescent protein that enables multichannel live imaging with minimal crosstalk and low photobleaching, suitable for applications like FRAP and laser ablation in Drosophila.

The paper identifies a short fluorescent protein isoform in mCherry that causes background fluorescence and proposes a modified version to improve reporter function.

The paper develops a technique to image human brain organoids at mesoscopic and super-resolution scales, revealing cellular and synaptic structures during development.

This paper reviews Fluorescence Fluctuation based Super Resolution Microscopy methods, explaining their principles and guiding their selection for live-cell imaging.

This paper introduces spectral fiber-photometry methods to correct hemoglobin absorption artifacts in fluorescent sensor recordings, improving accuracy in measuring neuronal activity.

This paper combines skull optical clearing and three-photon microscopy to achieve deep brain imaging through an intact mouse skull.

This study demonstrates isotropic expansion of the nucleus using expansion microscopy to investigate nanoscale DNA damage response structures.

This paper introduces globLoc, a GPU-based global fitting algorithm for multi-channel single-molecule localization, improving 3D precision and color assignment in SMLM.

Minian is an open-source pipeline for analyzing single-photon in vivo calcium imaging data, offering low memory usage, interactive visualizations, and parameter exploration tools.

This study uses a fluorescent nanosensor paint to show that dopamine release from individual varicosities is highly heterogeneous and requires molecular priming.

This paper develops a method to 3D molecularly characterize cleared human brain tissues using light-sheet fluorescence microscopy.

The paper introduces a fast particle fusion method for single-molecule localization microscopy that reduces computational cost from N² to linear scaling by using joint registration of multiple point clouds.

The paper evaluates tdTomato and mScarlet fluorescent proteins for dual-color three-photon microscopy in the mouse brain, finding high signal-to-background in specific wavelength ranges.

This paper develops an optically cleared intervertebral window for long-term, high-resolution, inflammation-free in vivo imaging of mouse spinal cord dynamics.

The paper introduces pixOL, a method that improves the precision of measuring 3D orientation and localization of fluorescent molecules using engineered dipole-spread functions.

FRET-IBRA processes large FRET image stacks with flexibility and speed, providing accurate ratio images and background correction while maintaining high signal-to-noise ratio.

This paper introduces a novel single objective light-sheet microscope that achieves high resolution, large field of view, and multi-view imaging for various biological systems.

This paper introduces a lightweight two-photon microscope for imaging over 1,000 neurons in freely moving mice, enabling large-scale calcium imaging with high resolution and minimal behavioral disruption.