Advanced Fluorescence Microscopy Techniques

The paper develops a spatially-detailed regression model to separate hemodynamic signals from GCaMP fluorescence in widefield imaging of awake mice, improving accuracy near blood vessels and along the sagittal midline.

The paper develops a method to simultaneously image local microcircuits and whole cortical activity in awake mice, and uses it to study neuron associations with network motifs.

The paper develops an algorithm to estimate fluorescent molecule numbers in single cells using photobleaching fluctuations, validated in yeast with Bayesian inference and noise modeling.

This paper introduces dual-plane 3-photon microscopy with remote focusing, enabling faster in vivo calcium imaging of two cortical planes in mice.

This paper introduces tapered optical fibers for efficient and selective light collection from deep brain tissues, enabling multisite photometry and customizable collection volumes.

The paper uses phasor-FLIM to assess pre-implantation embryo quality by analyzing fluorescence lifetime, achieving 86% accuracy in identifying healthy embryos.

This study optimizes and validates a hydrogel-based brain tissue clearing protocol, showing uniform expansion across anatomical regions and spatial scales.

The paper introduces a new genetically encoded voltage indicator, Voltron, which improves brightness and photostability for extended in vivo voltage imaging in mice, zebrafish, and fruit flies.

This paper introduces a two-photon imaging technique that achieves high-speed, high-resolution imaging of neural activity in mice at over 1 kHz frame rates.

The paper introduces MASH, a method for cytoarchitectonic labeling and optical clearing of human cortex samples, enabling scalable 3D characterization of neocortical cytoarchitecture.

The paper introduces a GFP-based cGMP sensor, WincG2, for real-time imaging of cGMP dynamics in intact C. elegans, showing its sensitivity to cGMP changes and responses to NaCl and SDS.

This paper develops a genetically encoded fluorescent sensor, iGABASnFR, to monitor GABA in vivo and demonstrates its use in tracking GABA dynamics in various neural contexts.

ColiCoords is a Python package that analyzes bacterial fluorescence microscopy data by transforming Cartesian coordinates to cellular coordinates, enabling statistical analysis of single-cell data.

The paper introduces a wearable neuroimager for whole-brain imaging in mice, revealing premotor activity across the motor cortex.

This study develops a dual-color fluorescent transcriptional timer to monitor spatio-temporal gene expression dynamics in Drosophila, identifying new genes with dynamic expression patterns.

The paper quantifies very low-abundant proteins in bacteria using HaloTag and epifluorescence microscopy, achieving ≥80% detection efficiency.

This paper develops a dual-color two-photon microscope for 3D volumetric imaging of neural activity in awake mice, revealing new insights into cortical layer interactions and functional connectivity.

This paper introduces a tomography method for fast volume imaging using Bessel beams and neural networks, suitable for dynamic samples like neural activity.

This paper introduces a light-sheet microscopy method for fast, large-scale volumetric brain imaging at synaptic resolution, achieving whole mouse brain scans in 2 hours.

WindSTORM improves high-throughput nanoscopy by enabling fast and accurate emitter localization with robust background correction.

This study compares neuronal response measurements from miniaturized microscopes and two-photon calcium imaging in mouse visual cortex, finding strong correlations in response amplitude, signal-to-noise ratio, and orientation tuning.

The paper develops GRABNE sensors for rapid, specific in vivo detection of norepinephrine, demonstrating their use in various experimental contexts.

This paper introduces a progressive refinement method (PRIS) for 3D high-density super-resolution imaging using sparse recovery, demonstrating its effectiveness with various PSF types and multi-channel observations.

The paper characterizes 27 fluorescent proteins in vivo in yeast, finding differences from in vitro data and showing how codon optimization affects their properties.

MemBright is a family of fluorescent membrane probes that enable high-contrast, selective plasma membrane staining for advanced cellular imaging and neuroscience applications.

This paper demonstrates the feasibility of two-photon frequency division multiplexing (2P-FDM) for recording calcium signals in brain tissue, showing a significant reduction in cross-talk and improved signal quality.

NanoJ provides a high-performance open-source toolbox for super-resolution microscopy, offering tools for data alignment, image reconstruction, quality assessment, structural modeling, and sample control.

This paper develops a method for high fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins and light-sheet imaging.

The paper combines expansion and lattice light sheet microscopy to enable high-speed, nanoscale molecular imaging of neural circuits across large volumes.

The paper introduces an automated Bayesian pipeline for analyzing single-molecule binding data and benchmarks it using TtAgo binding properties.