Glycosylation and Glycoproteins Research

Excessive O-GlcNAcylation causes heart failure and sudden death

This paper shows that excessive O-GlcNAcylation causes heart failure and sudden death in mice, while reducing it protects against cardiac stress.

Expanding N-Glycopeptide Identifications by Fragmentation Prediction and Glycome Network Smoothing

The paper improves N-glycopeptide identification using fragmentation prediction and glycome network smoothing, demonstrating performance gains on existing datasets.

OGP: A Repository of Experimentally Characterized O-Glycoproteins to Facilitate Studies on O-Glycosylation

OGP provides a comprehensive repository of O-glycoproteins and a prediction tool to facilitate O-glycosylation studies.

Precise, Fast and Comprehensive Analysis of Intact Glycopeptides and Modified Saccharide Units with pGlyco3

pGlyco3 is a glycan-first search engine for analyzing glycopeptides and modified saccharide units, offering faster performance without sacrificing accuracy.

O-GlcNAcAtlas: A Database of Experimentally Identified O-GlcNAc Sites and Proteins

O-GlcNAcAtlas compiles experimentally identified O-GlcNAc sites and proteins, providing a curated database for functional and computational studies.

A Drosophila natural variation screen identifies NKCC1 as a substrate of NGLY1 deglycosylation and a modifier of NGLY1 deficiency

SugarPy facilitates the universal, discovery-driven analysis of intact glycopeptides

SugarPy enables glycoproteome analysis without glycan databases, validated on human breast milk and other organisms with uncommon glycans.

Ethanolamine phosphate on the second mannose as bridge in GPI anchored proteins: Towards understanding inherited PIGG deficiency

The paper shows EthN-P on Man2 is the preferential bridge for GPI-AP attachment, challenging the current view of GPI anchoring and explaining PIGG deficiency mechanisms.

O-Pair Search with MetaMorpheus for O-glycopeptide Characterization

O-Pair Search identifies O-glycopeptides and localizes O-glycosites using collision- and electron-based dissociation spectra, improving speed and identification rates.

Fast and Comprehensive N- and O-glycoproteomics analysis with MSFragger-Glyco

MSFragger-Glyco improves glycopeptide identification in N- and O-glycoproteomics data, increasing glycoPSMs and revealing new glycan trends.

Assembly Mechanism of Mucin and von Willebrand Factor Polymers

The paper presents an integrated structural analysis of intestinal mucin MUC2, revealing the shared mechanism of polymer formation for mucosal barriers and blood clotting.

Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation

This paper introduces GalNAzMe, a monosaccharide probe that selectively labels O-GalNAc glycosylation in living cells, enabling detailed study of this cancer-relevant modification.

Unliganded and CMP-Neu5Ac bound structures of human α-2,6-sialyltransferase ST6Gal I at high resolution

The paper presents two high-resolution structures of human ST6Gal I, one unliganded and one with CMP-Neu5Ac bound, revealing structural flexibility and substrate binding details.

Novel Serine/Threonine-O-glycosylation with N-Acetylneuraminic acid and 3-Deoxy-D-manno-octulosonic acid by Maf glycosyltransferases

This paper identifies two novel O-glycosylation modifications on bacterial flagellins catalyzed by Maf glycosyltransferases in Geobacillus kaustophilus and Clostridium botulinum.

GlypNirO: An automated workflow for quantitative N- and O-linked glycoproteomic data analysis

GlypNirO automates quantitative glycoproteomic analysis by integrating Byonic and Proteome Discoverer outputs, identifying glycosylation changes in hepatocellular carcinoma as potential biomarkers.

Mass spectrometry analysis of newly emerging coronavirus HCoV-19 spike S protein and human ACE2 reveals camouflaging glycans and unique post-translational modifications

The paper uses mass spectrometry to analyze post-translational modifications and glycans of SARS-CoV-2 spike protein and hACE2, revealing structural details relevant to virus-host interactions and vaccine development.

Sialylation of Asparagine 612 inhibits Aconitase activity during mouse sperm capacitation; A possible mechanism for the switch from oxidative phosphorylation to glycolysis

The paper identifies sialylation changes in mouse sperm during capacitation, showing that sialylation of ACO2 inhibits its activity, potentially driving the metabolic switch from oxidative phosphorylation to glycolysis.

Sialylation and fucosylation changes of cytidine monophosphate-Nacetylneuraminic acid hydroxylase (CMAH) and glycoprotein, alpha1,3-galactosyltransferase(GGTA1) knockout pig erythrocyte membranes

The paper analyzes glycosylation changes in GGTA1/CMAH DKO pig RBCs using mass spectrometry and lectin blotting, finding reduced sialylation and fucosylation.

Enrichment-Free Identification of Native Definitive (EnFIND) O-glycoproteome of antibodies in autoimmune diseases

This paper introduces an enrichment-free method to analyze native O-glycoproteomes, revealing high O-glycosylation in antibodies from autoimmune diseases.

Examining and fine-tuning the selection of glycan compositions with GlyConnect Compozitor

The paper introduces GlyConnect Compozitor, a tool for visualizing and comparing glycan compositions to aid in selecting appropriate compositions for glycoproteomics analysis.

Systematic evaluation of normalization methods for glycomics data based on performance of network inference

The paper evaluates normalization methods for glycomics data by assessing how well they enable accurate reconstruction of known glycosylation pathway reactions using Gaussian Graphical Models.

Algorithmic biosynthesis of eukaryotic glycans

The paper resolves the paradox of glycan microheterogeneity by developing algorithmic compartments that convert inputs to unique outputs, and applies this to real glycan datasets.

The Function Annotations Of ST3GAL4 In Human LAMP1 And Lassa Virus GP-C Interaction From The Perspective Of Systems Virology

This study investigates the role of ST3GAL4 in the interaction between Lassa virus GP-C and human LAMP1, identifying sialylated glycans as mediators of viral entry.

Synaptic transmission induces site-specific changes in sialylation on N-linked glycoproteins in rat nerve terminals

This study identifies site-specific changes in sialylation of N-linked glycoproteins in rat nerve terminals following brief depolarization.

Assignment of coronavirus spike protein site-specific glycosylation using GlycReSoft

The paper demonstrates the use of GlycReSoft to analyze site-specific glycosylation of the SARS-CoV-2 spike protein from glycoproteomics data.

Off-target glycans encountered along the synthetic biology route towards humanized N-glycans in Pichia pastoris

The paper identifies off-target glycans formed during the engineering of Pichia pastoris to produce humanized N-glycans, highlighting challenges in achieving precise glycosylation control.

Chemical precision glyco-mutagenesis by glycosyltransferase engineering in living cells

The paper introduces a method to chemically tag glycans in living cells using engineered glycosyltransferases to study glycan structure and biosynthesis.

Mutually exclusive locales for N-linked glycans and disorder in glycoproteins

The paper analyzes N-linked glycosylation sites in human glycoproteins and finds they are more prevalent in ordered regions than disordered regions, suggesting a functional trade-off between protein stability and flexibility.

SweetOrigins: Extracting Evolutionary Information from Glycans

This paper develops a deep learning model to extract evolutionary information from glycans, revealing insights into host-pathogen interactions and phenotypic diversity.

Deep evolutionary analysis reveals the design principles of fold A glycosyltransferases

The paper uses evolutionary analysis of GT-A fold sequences to identify core components and hypervariable loops contributing to catalytic diversity, and develops a machine learning classifier for donor specificity prediction.