Glycosylation and Glycoproteins Research

Algorithmic biosynthesis of eukaryotic glycans

The paper resolves the paradox of glycan microheterogeneity by developing algorithmic compartments that convert inputs to unique outputs, and applies this to real glycan datasets.

The Function Annotations Of ST3GAL4 In Human LAMP1 And Lassa Virus GP-C Interaction From The Perspective Of Systems Virology

This study investigates the role of ST3GAL4 in the interaction between Lassa virus GP-C and human LAMP1, identifying sialylated glycans as mediators of viral entry.

Synaptic transmission induces site-specific changes in sialylation on N-linked glycoproteins in rat nerve terminals

This study identifies site-specific changes in sialylation of N-linked glycoproteins in rat nerve terminals following brief depolarization.

Assignment of coronavirus spike protein site-specific glycosylation using GlycReSoft

The paper demonstrates the use of GlycReSoft to analyze site-specific glycosylation of the SARS-CoV-2 spike protein from glycoproteomics data.

Off-target glycans encountered along the synthetic biology route towards humanized N-glycans in Pichia pastoris

The paper identifies off-target glycans formed during the engineering of Pichia pastoris to produce humanized N-glycans, highlighting challenges in achieving precise glycosylation control.

Chemical precision glyco-mutagenesis by glycosyltransferase engineering in living cells

The paper introduces a method to chemically tag glycans in living cells using engineered glycosyltransferases to study glycan structure and biosynthesis.

Mutually exclusive locales for N-linked glycans and disorder in glycoproteins

The paper analyzes N-linked glycosylation sites in human glycoproteins and finds they are more prevalent in ordered regions than disordered regions, suggesting a functional trade-off between protein stability and flexibility.

SweetOrigins: Extracting Evolutionary Information from Glycans

This paper develops a deep learning model to extract evolutionary information from glycans, revealing insights into host-pathogen interactions and phenotypic diversity.

Deep evolutionary analysis reveals the design principles of fold A glycosyltransferases

The paper uses evolutionary analysis of GT-A fold sequences to identify core components and hypervariable loops contributing to catalytic diversity, and develops a machine learning classifier for donor specificity prediction.

Glycoinformatics approach for identifying target positions to inhibit initial binding of SARS-CoV-2 S1 protein to the host cell

The paper predicts O-glycosylation and phosphorylation sites on SARS-CoV-2 S1 protein to identify potential targets for inhibiting initial host cell binding.

Detecting substrate glycans of fucosyltransferases on glycoproteins with fluorescent fucose

The paper detects substrate glycans of fucosyltransferases using fluorescent fucose incorporation to study their specificity and interactions with sialylation.

N-linked surface glycan biosynthesis, composition, inhibition, and function in cnidarian-dinoflagellate symbiosis

This study investigates N-glycan biosynthesis in the dinoflagellate Breviolum minutum and shows that inhibiting Golgi processing reduces its colonization of the sea anemone Aiptasia.

Using Natural Language Processing to Learn the Grammar of Glycans

This paper develops a language model for glycans, SweetTalk, to analyze substructure motifs, classify linkages, and predict immunogenicity with 92% accuracy.

A Chemoenzymatic Method for Glycoproteomic N-glycan Type Quantitation

The paper introduces a chemoenzymatic method to quantitatively analyze N-glycan types on glycoproteins, demonstrating its application in characterizing glycan heterogeneity in IgG1 and MCF7 cells.

A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

The paper introduces a cell-free platform for assembling protein glycosylation pathways and demonstrates its use in creating vaccine proteins and antibody constant regions with specific glycan motifs.

Multilayered N-glycoproteomics reveals impaired N-glycosylation promoting Alzheimer’s disease

This study uses multilayered N-glycoproteomics to show impaired N-glycosylation in Alzheimer's disease mouse models, affecting protein function and neuronal health.

EXoO-Tn: Tag-n-Map the Tn Antigen in the Human Proteome

The paper introduces EXoO-Tn, a method to map Tn-glycosylation sites in the human proteome, identifying 947 sites from 480 glycoproteins in Jurkat cells.

Mammalian Y RNAs are modified at discrete guanosine residues with N-glycans

The paper identifies N-glycan modifications on mammalian Y RNAs, showing RNA can be a scaffold for glycosylation in the secretory pathway.

Genetic and functional evidence relates a missense variant in B4GALT1 to lower LDL-C and fibrinogen

The paper identifies a missense variant in B4GALT1 associated with lower LDL-C and fibrinogen, supported by genetic, functional, and glycan profiling evidence.

Correcting for sparsity and non-independence in glycomic data through a systems biology framework

The paper introduces GlyCompare, a glycomic analysis method that addresses sparsity and non-independence by leveraging biosynthetic pathways, revealing biological insights in glycoprofiles and human milk oligosaccharides.

Altered protein expression of galactose and N-acetylgalactosamine transferases in schizophrenia superior temporal gyrus

The paper investigates protein expression levels of galactose and N-acetylgalactosamine transferases in schizophrenia brain tissue and identifies reduced B4GALT1 and GALNT16.

Capturing site-specific heterogeneity with large-scale N-glycoproteome analysis

This study captures site-specific N-glycoproteome heterogeneity using AI-ETD, identifying 1,545 N-glycosites from mouse brain tissue and developing new visualizations for glycopeptide analysis.

Defining the genetic control of human blood plasma N-glycome using genome-wide association study

This study identifies twelve genetic loci associated with human blood plasma N-glycome composition through genome-wide association analysis, revealing both direct and indirect genetic influences on glycosylation.

Mapping In Vivo O-Glycoproteome Using Site-specific Extraction of O-linked glycopeptides (EXoO)

The paper introduces EXoO, a method for site-specific extraction of O-linked glycopeptides, enabling the mapping of over 3,000 O-linked glycosylation sites across human tissues and revealing significant differences between tumor and normal kidney tissues.

e-workflow for recording of glycomic mass spectrometric data in compliance with reporting guidelines

The paper describes an e-infrastructure for recording glycomic mass spectrometric data in compliance with MIRAGE reporting guidelines, enabling standardized data submission to a public repository.

Increased α-2,8-sialyltransferase 8B (ST8SIA2) expression in schizophrenia superior temporal gyrus

The paper finds increased ST8SIA2 protein expression in schizophrenia superior temporal gyrus, suggesting a potential compensatory mechanism for impaired PSA-NCAM synthesis.

Glycosylation enzyme mRNA expression in dorsolateral prefrontal cortex of elderly patients with schizophrenia: Evidence for dysregulation of multiple glycosylation pathways

The paper identifies dysregulated mRNA expression of 36 glycosylation enzymes in schizophrenia patients, suggesting disrupted glycosylation pathways contribute to the disorder.

Reanalysis of global proteomic and phosphoproteomic data identified a large number of glycopeptides

The paper reanalyzes proteomic and phosphoproteomic data to identify a large number of previously unknown N-linked glycopeptides using GPQuest 2.0.

N-linked glycosylation of the antagonist Short gastrulation increases the functional complexity of BMP signals

The paper shows that N-glycosylation of Sog in Drosophila affects BMP signaling by controlling extracellular Sog levels and distribution, adding complexity to BMP regulation.

A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases

This paper presents a cell-free platform for synthesizing active oligosaccharyltransferases (OSTs) and demonstrates its ability to produce and test multiple OSTs for glycosylation reactions.