Advanced Electron Microscopy Techniques and Applications

The paper introduces FlexConsensus, an algorithm that merges conformational landscapes from different CryoEM heterogeneity methods into a shared consensus space to improve reliability and reproducibility.

The paper introduces a modification to cryo-FIB milling patterns by adding fillets to reduce stress concentration and lamella breakage, improving yield by 33%.

This paper introduces tilt-corrected bright-field scanning-TEM (tcBF-STEM) for cryo-EM, showing improved contrast and efficiency for thick samples, with a subnanometer resolution SPA map of a virus-like particle.

The paper introduces a method to separate atomic displacement and local resolution effects in crystallographic and cryo-EM maps by modeling Fourier ripples.

The paper investigates using p-values to estimate the number of distinguishable classes in cryoEM data by testing if classifications differ from random partitions.

This paper introduces a genetically encoded FerriTag system for labeling target proteins in live cells for cryo-electron tomography, enabling precise localization with high contrast.

This paper introduces a method to validate 3D cryoEM reconstructions by separating scattering contributions into real and imaginary components to determine handedness and assess reconstruction quality.

This paper introduces a method to reduce preferred orientation in cryo-EM samples using laser flash melting and revitrification, improving protein structure determination.

The paper validates helical symmetry parameters in EMDB by identifying and correcting inconsistencies between parameters and density maps.

Magellon provides a flexible and scalable cryo-EM platform with microservices architecture for data management, processing, and visualization.

This study uses cryogenic light microscopy to determine three conformational states of PIEZO1 in the cell membrane, revealing structural changes associated with channel opening.

The paper uses cryo-EM to analyze membrane proteins, revealing unexpected high-resolution structures of ArnA and AcrB instead of the intended FtsH and YidC complex.

The paper introduces Di-Gluebodies, covalently-dimerized nanobodies, to improve cryo-EM structure determination of small proteins by providing rigidity and modularity.

The paper uses cryoEELS and cryoET to study calcium distribution in mitochondria of human retinal ganglion cells under calcifying conditions, revealing mitochondrial calcification.

This paper demonstrates that a 100 keV cryo-TEM with advanced components can achieve sub-3 Å resolution structures of various proteins and viruses.

The paper introduces AIVE, an AI-assisted method for segmenting membrane boundaries in vEM data, revealing a new type of mitochondrial contact called intrusions.

The paper determines the atomic structure of the PS2 S-layer from Corynebacterium glutamicum using cryo-EM and shows its polar assembly mechanism and phylogenetic distribution.

EMSequenceFinder accurately assigns amino acid sequences to cryo-EM map backbone fragments, outperforming existing methods in resolution 3-10 Å maps.

This paper introduces EMERALD-ID, a method for identifying small molecules in cryo-EM data, achieving 43% exact identification and 66% close identification of common ligands.

This study investigates how cooling temperature, immersion medium, and EM grid material affect devitrification in cryo-SMLM, showing that metallic supports in cold nitrogen tolerate higher laser intensities.

This study develops a deep learning method to automatically remove corrupted tilts in cryo-ET, showing high efficiency and reliability with trained models for direct application.

The paper introduces and analyzes Q-scores to assess the reliability of atomic coordinate models fitted to 3DEM maps, showing their correlation with map resolution and model quality.

This paper introduces a foam film vitrification method for cryo-EM that improves ice thickness control and particle orientation, simplifying specimen preparation.

This study determines the structure of the complete extracellular bacterial flagellum, revealing mechanisms of flagellin incorporation and filament assembly.

The paper determines the in situ structure of a high-torque bacterial flagellar motor using cryogenic electron microscopy, revealing protein adaptations and universal principles of torque generation.

This paper develops an ultrafast pulsed ponderomotive phase plate to enhance contrast in cryo-ET by synchronizing electron packets with laser pulses.

The paper introduces cryoDRGN-AI, a method for ab initio 3D reconstruction of cryo-EM and cryo-ET datasets, revealing new conformational states and unresolved motions.

The paper characterizes a two-component encapsulin from Streptomyces lydicus, showing differential assembly and mixed shell formation with irregular tiling.

The paper compares CEMOVIS and FIB-milling for cryo-EM sample preparation, showing CEMOVIS provides more imageable area with comparable structural preservation.

Xenon plasma FIB cryo lift-out from high-pressure-frozen mouse hippocampal slices up to 200 µm thick enables fluorescence-guided cryoET of CA1 synapses and apical dendrite cytoskeleton.