Holger Gerhardt

Neuroblastoma-derived small extracellular vesicles retaincellular identity and adrenergic/mesenchymal state signatures

This study shows that neuroblastoma-derived sEVs retain genomic subgroup and lineage state signatures from their parental cells.

Tunable Laminar Perfusion Coordinates Endothelial and Perivascular Remodeling in Angiogenic Vasculature-on-Chip

This study introduces a tunable laminar perfusion system for vasculature-on-chip to study sustained flow effects on vascular remodeling and immune interactions.

Self-organized vascularized human liver spheroids: Serum-free culture conditions and use as tissue building blocks

The paper describes a method to generate vascularized human liver spheroids using specific cell types and media, enabling their use as tissue building blocks.

Gene-specific endothelial programs drive AVM pathogenesis in SMAD4 and ALK1 loss-of-function

This study identifies gene-specific mechanisms of AVM formation in endothelial cells with SMAD4 or ALK1 loss-of-function, showing distinct molecular and cellular pathways leading to different vascular malformations.

Endothelial YAP/TAZ rewiring under cardiometabolic stress drives sex-divergent vascular remodeling in heart failure with preserved ejection fraction

This study identifies endothelial YAP/TAZ signaling as a driver of sex-divergent vascular remodeling in HFpEF, linking cardiometabolic stress to microvascular changes through YAP1 and ESM1.

Disrupted endothelial cell-cell dynamics in ALK1 and SMAD4 deficiency drive arteriovenous malformations

This study shows that ALK1 and SMAD4 deficiencies disrupt endothelial cell interactions, leading to arteriovenous malformations through distinct biomechanical mechanisms.

Isogenic monocytes improve the responsiveness of hiPSC cardiac spheroids to cardiac stressors

The paper develops a cardiac spheroid model with monocytes to better mimic heart failure responses, showing improved functional and transcriptional changes compared to models without immune cells.

Connexin 41.8 mediates the correct temporal induction of haematopoietic stem and progenitor cells

This study shows that Cx41.8 regulates the timing of mitochondrial ROS production, which stabilizes the Hif pathway to enable proper haemogenic endothelium specification and HSPC induction in zebrafish.

Mechanosensitive PIEZO2 channels shape coronary artery development

This study shows PIEZO2 channels are critical for coronary artery development, as their absence causes vessel malformations and cardiac issues in mice.

Polarity-JaM: An image analysis toolbox for cell polarity, junction and morphology quantification

Polarity-JaM is an open-source image analysis tool for quantifying cell polarity, junctions, and morphology using deep learning and provides statistical analysis features.

Mechanical Strain Activates Planar Cell Polarity Signaling to Coordinate Vascular Cell Dynamics

This study identifies Vangl1 as a key mediator of flow-dependent cell dynamics in vascular remodeling through planar cell polarity signaling.

Pial collaterals develop through mosaic colonization of capillaries by arterial and microvascular endothelial cells

This study identifies a novel mechanism for pial collateral development in embryos and ischemic remodeling in mice, showing distinct roles of arterial and microvascular endothelial cells.

Reconstructing stochastic cell population trajectories reveals regulators and heterogeneity of endothelial flow-migration coupling driving vascular remodelling

The paper develops a computational model to analyze EC migration during vascular remodelling, finding that directional migration is driven by dual force fields and mediated by Cdc42 with cell population heterogeneity.

Coronary vessel assembly involves patterned endocardial sprouting and tip-cell-to artery specification

This study identifies endocardial sprouting angiogenesis as a key mechanism in coronary vessel formation, revealing distinct molecular profiles that shape coronary arteries and veins.

Svep1 stabilizes developmental vascular anastomosis in reduced flow conditions.

This study identifies Svep1 as a factor that stabilizes vascular anastomosis in zebrafish embryos under reduced flow conditions.

Wasp controls oriented migration of endothelial cells to achieve functional vascular patterning

The paper shows that WASp regulates endothelial cell migration directionality in zebrafish, leading to proper vascular patterning and preventing vascular malformations.

Association between erythrocyte dynamics and vessel remodelling in developmental vascular networks

The paper investigates how erythrocyte dynamics influence vascular remodelling in developing networks, finding that RBC depletion correlates with vessel regression and that plasma skimming drives this process.

Vasohibin-1 mediated tubulin detyrosination selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk

This study shows that Vasohibin-1 mediates tubulin detyrosination in zebrafish endothelial cells, selectively regulating secondary sprouting and lymphangiogenesis in the trunk.

PolNet Analysis: a software tool for the quantification of network-level endothelial cell polarity and blood flow during vascular remodelling

PolNet quantifies endothelial cell polarity and blood flow in vascular networks using image analysis and computational fluid dynamics.

YAP and TAZ regulate adherens junction dynamics and endothelial cell distribution during vascular development

This study shows YAP and TAZ regulate endothelial cell dynamics and vascular development by modulating junctions and signaling pathways.