Robert C. Spitale

Upregulation of Calbindin in Adult Inhibitory Neurons Reactivates Critical Period Plasticity in Mouse Visual Cortex

Quantification of subcellular RNA localization through direct detection of RNA oxidation

This paper introduces OINC-seq, a method to quantify subcellular RNA localization through direct oxidation detection, and demonstrates its application in various cellular compartments and live zebrafish.

DNA G-quadruplex is a transcriptional control device that regulates memory

The paper shows DNA G-quadruplex regulates gene expression in neurons, affecting memory formation and fear extinction.

The anti-cancer compound JTE-607 reveals hidden sequence specificity of the mRNA 3' processing machinery

The paper identifies sequence-specific sensitivity to JTE-607 in mRNA 3' processing by analyzing over 260,000 sequence variants and developing a predictive model.

Fear extinction is regulated by long noncoding RNA activity at the synapse

The paper identifies a lncRNA, Gas5, involved in fear extinction by regulating RNA granule trafficking and clustering at synapses in mice.

Aberrant splicing in Huntington's disease via disrupted TDP-43 activity accompanied by altered m6A RNA modification.

This study identifies TDP-43 dysfunction and reduced m6A modification as mechanisms causing aberrant splicing in Huntington's disease using mouse and human brain data.

Discovery of synapse-specific RNA N6-methyladenosine readers associated with the consolidation of fear extinction memory

The paper identifies synapse-specific m6A RNA readers and their role in fear extinction memory consolidation through RNA immunoprecipitation and sequencing in mice.

Analysis of subcellular transcriptomes by RNA proximity labeling with Halo-seq

This study introduces Halo-seq, a method for quantifying subcellular RNA populations, and finds that AU-rich elements are enriched in the nucleus and their export depends on HuR.

Identification of novel regulators of dendrite arborization using cell type-specific RNA metabolic labeling

The paper uses EC-tagging to profile md neuron transcriptomes and identifies two RNA-binding proteins involved in dendrite arborization.

Long-TUC-seq is a robust method for quantification of metabolically labeled full-length isoforms

This paper introduces Long-TUC-seq, a method combining metabolic labeling with long-read sequencing to reliably detect isoform-level transcription dynamics.

A technology-agnostic long-read analysis pipeline for transcriptome discovery and quantification

TALON annotates and counts full-length reads from PacBio or Nanopore in one framework; in mouse brain, 422 genes showed more novel-isoform reads than annotated ones.

Functional conservation of lncRNA JPX despite sequence and structural divergence

The paper investigates the functional conservation of lncRNA JPX across species despite sequence and structural divergence, showing it can functionally complement Jpx in mouse cells.

The accumulation of N6-methyl-2-deoxyadenosine in DNA drives activity-induced gene expression and is required for the extinction of conditioned fear

This study shows that m6dA accumulation in DNA of prefrontal cortical neurons is linked to fear extinction learning and gene expression.