DNA Repair Mechanisms

This study uses a CRISPR-based method to investigate ecDNA formation, showing efficient circularization and distinct repair pathways for ecDNA and chromosomal scars.

The paper reports the crystal structure of MEILB2-BRME1 complex and shows how BRCA2 binding causes it to form a V-shaped DNA clamp to facilitate meiotic recombination.

This study identifies the role of ATM and 53BP1 in regulating alternative end joining-mediated V(D)J recombination by analyzing repair outcomes of RAG1/2-initiated DSBs.

This study identifies RBM10's role in DNA replication and shows that targeting WEE1 can sensitize RBM10-deficient lung adenocarcinoma cells.

This study investigates DNA damage tolerance mechanisms in Drosophila melanogaster, showing that translesion synthesis is preferred over template switching for alkylation damage, with REV1 playing a critical role in coordinating this response.

The paper identifies mechanisms that limit Ku protein accumulation in chromatin, showing DNA-PKcs blocks Ku entry, and two additional mechanisms restrict Ku levels in the absence of DNA-PKcs.

The paper shows that germ cells in Drosophila tolerate DNA damage during meiosis but are sensitive to it during mitosis, revealing a threshold for DNA damage tolerance in germline development.

This study compares the localization and dynamics of the TLS polymerase Pol Y1 in Bacillus subtilis with its E. coli homolog Pol IV, revealing differences in their regulation and replication machinery association.

This study shows that CMG helicase disassembly is essential in budding yeast and occurs via two pathways involving ubiquitylation and Rrm3/Pif1 helicases.

The paper uses XR-seq to map transcription events in C. elegans by analyzing repair in XPC mutants, revealing previously unrecognized transcription sites.

This study identifies a novel regulatory mechanism for WRN degradation via the PIAS4-RNF4-p97/VCP axis in MSI-H cancer cells using single molecule tracking and inhibitor screening.

MADDD-seq detects DNA damage and mutations simultaneously in yeast using a modified double barcoding sequencing approach, revealing repair kinetics and mutagenesis over 24 hours.

This study shows eEF2K is critical for the DNA damage response to cisplatin, promoting p53 activation and DNA repair depending on damage severity.

This study shows that prolonged RNA Polymerase II stalling at DNA damage explains the differing phenotypes between Cockayne Syndrome and UV-sensitive Syndrome.

This study shows that non-homologous end joining (NHEJ) is the main pathway causing complex genomic rearrangements during chromothripsis from mitotic errors.

The paper develops a method to map Pol κ activity across the genome in human fibroblasts and finds it is enriched in euchromatin, gene promoters, and early S-phase replicated DNA.

This paper identifies a novel role for Cyclophilin A in DNA repair via interaction with the MRN complex, showing that its PPI activity is critical for homologous recombination repair after replication fork stalling.

This study identifies a novel phosphorylation motif used by DNA-PKcs, revealing its role in regulating splicing factors and transcriptional regulators across species.

This study investigates the DNA binding and bridging activities of CtIP in healthy and diseased states, identifying a C-terminal DNA-binding domain and showing that tetramerization is required for DNA bridging.

The paper studies DNA double-strand breaks (DSBs) during nematode programmed DNA elimination, showing that DSBs are processed with end resection and telomere addition, with biased repair on retained ends.

A multiplexed screen reveals how dozens of proteins sense the local chromatin context to tune the balance between two DNA repair pathways.

This study shows that Decitabine's DNA damage response depends on DNMT activity levels, with different repair pathways activated under varying conditions.

The paper investigates how Srs2 helicase and Rrp1/Rrp2 translocases affect replication stress response in fission yeast, showing differential checkpoint activation with overproduction.

This study characterizes the multivalent interactions of XRCC4 and XLF CTRs, showing they form condensates that enhance DNA ligation in vitro and suggest roles in NHEJ dynamics and cancer biology.

This study shows that HSR-9, the C. elegans ortholog of 53BP1, regulates meiotic recombination and X chromosome segregation, with both conserved and unique functions compared to somatic cells.

The paper identifies Msc1 as a nuclear envelope protein that enhances DNA repair in late mitosis by supporting homologous recombination.

The paper investigates how strand-asymmetric processes like replication and transcription influence DNA damage, repair, and mutagenesis, revealing mechanisms that shape cancer genome evolution.

The paper uses single-molecule imaging to reveal how two CMG helicases are assembled and activated at replication origins in metazoan genomes.

The paper investigates how SUMO protease and proteasome recruitment at nuclear pores affects replication dynamics at arrested forks in fission yeast.

The paper examines RAD51 transcriptional response and cell cycle dynamics in Arabidopsis root cell types under DNA damage, revealing differences in sensitivity to genotoxic stress.