Bacterial biofilms and quorum sensing

Avirulent Pseudomonas aeruginosa T3SS-negative strains belonging to Clade 5 produce variable quantities of secondary metabolites

The paper characterizes avirulent P. aeruginosa strains lacking T3SS genes, showing they produce variable secondary metabolites despite low cytotoxicity.

Pleiotropic regulation of bacterial toxin production and Allee effect govern microbial predator-prey interactions

The paper investigates how bacterial toxin production and Allee effects influence predator-prey dynamics between Pseudomonas fluorescens and amoebae, revealing spatiotemporal regulation of toxin production.

Absence of biofilm adhesin proteins changes surface attachment and cell strategy for Desulfovibrio vulgaris Hildenborough

This study shows that absence of two biofilm adhesins in Desulfovibrio vulgaris Hildenborough affects surface attachment and cell strategy, with distinct roles compared to other bacteria.

Surfactin accelerates Bacillus subtilis pellicle biofilm development

This study investigates the role of surfactin in pellicle biofilm formation in B. subtilis, finding that surfactin deletion delays pellicle formation, which is rescued by exogenous surfactin.

Pseudomonas aeruginosa Alkyl Quinolone Response is dampened by Enterococcus faecalis

This paper shows that Enterococcus faecalis reduces Pseudomonas aeruginosa's production of quinolone signals and pyocyanin through ornithine, enabling mutualistic survival in co-culture.

Long-term adaptation to hypoxia provides insight into mechanisms facilitating the switch of Pseudomonas aeruginosa to chronic lung infections

This study shows that long-term hypoxia exposure leads to stable phenotypic and proteomic changes in Pseudomonas aeruginosa, similar to those observed in chronic CF lung infections.

The conserved global regulator H-NS has a strain-specific impact on biofilm formation in Vibrio fischeri symbionts

This paper shows that the conserved regulator H-NS has strain-specific effects on biofilm formation in Vibrio fischeri, with different strains showing varied phenotypes when hns is disrupted.

The Fem Cell-surface Signaling System is Regulated by ExsA in Pseudomonas aeruginosa and Affects Pathogenicity

The paper shows that ExsA regulates the Fem system in Pseudomonas aeruginosa, linking it to virulence factors and affecting pathogenicity in vivo.

New tools to monitor Pseudomonas aeruginosa infection and biofilms in vivo in C. elegans.

This paper develops tools to study Pseudomonas aeruginosa infection and biofilm formation in vivo using C. elegans, identifying RhlR as a potential antimicrobial target.

The Histidine Kinase NahK Regulates Denitrification and Nitric Oxide Accumulation through RsmA inPseudomonas aeruginosa

This study identifies NahK as a regulator of denitrification and NO accumulation in P. aeruginosa, linking it to cell morphology changes through RsmA.

Exploring aggregation genes in a P. aeruginosa chronic infection model

This study identifies genes essential for Pseudomonas aeruginosa aggregate formation in CF sputum using RNA-seq and microscopy, revealing distinct spatial phenotypes and cooperative interactions.

Anti-biofilm activity of new low molecular weight compounds produced by Lactiplantibacillus plantarum SJ33 against Klebsiella pneumoniae

This study identifies two new low molecular weight compounds, C1 and C2, produced by Lactiplantibacillus plantarum SJ33 that exhibit antibacterial and anti-biofilm activity against Klebsiella pneumoniae.

Serotype switching in Pseudomonas aeruginosa ST111 enhances adhesion and virulence

This study shows that serotype switching in Pseudomonas aeruginosa ST111 from O4 to O12 enhances adhesion, motility, and virulence in murine pneumonia.

Biofilm dispersal patterns revealed using far-red fluorogenic probes

This study uses far-red fluorogenic probes to reveal single-cell dispersal patterns in Vibrio cholerae biofilms, showing that dispersal starts at the periphery and 25% of cells never disperse.

RetS senses neurotransmitter norepinephrine and adrenergic agents to regulate Pseudomonas aeruginosa virulence

The paper identifies RetS as a bacterial adrenergic receptor in P. aeruginosa that responds to norepinephrine and dronedarone, modulating virulence and pathogenesis.

Bacterial single-cell RNA sequencing captures biofilm transcriptional heterogeneity and differential responses to immune pressure

This study develops BaSSSh-seq, a bacterial single-cell RNA sequencing method, to analyze transcriptional heterogeneity and immune responses in biofilms.

Adhesive interactions within microbial consortia can be differentiated at the single-cell level through expansion microscopy

This study uses expansion microscopy to differentiate adhesive interactions between microbial cells in biofilms at the single-cell level.

An in-depth analysis and exploreation with focus on the biofilm in Staphylococcus aureus

This study analyzes RNA sequencing data on Staphylococcus aureus biofilm formation using machine learning and co-expression analysis to identify functional modules and novel protein interactions, and develops a database for storing and analyzing the data.

Structures of the multi-domain oxygen sensor DosP: remote control of a c-di-GMP phosphodiesterase by a regulatory PAS domain

The paper determines six structures of DosP, revealing how its heme-binding PAS domain regulates c-di-GMP phosphodiesterase activity through allosteric mechanisms.

Pseudomonas aeruginosa essential gene perturbations that confer vulnerability to the mammalian host environment

This study identifies Pseudomonas aeruginosa genes whose partial knockdown increases vulnerability in a murine pneumonia model, highlighting potential targets for antibacterial strategies.

Quorum sensing orchestrates parallel cell death pathways in Vibrio cholerae via Type 6 secretion dependent and independent mechanisms

The paper identifies vca0646-0649 genes regulated by HapR that induce cell death in Vibrio cholerae independently of the T6SS system, revealing a second parallel cell death pathway.

Interspecies interactions alter the antibiotic sensitivity of Pseudomonas aeruginosa

This study quantifies how interspecies interactions affect antibiotic sensitivity of Pseudomonas aeruginosa in polymicrobial infections and shows these interactions can alter treatment effectiveness.

Polymicrobial interactions between Staphylococcus aureus and Pseudomonas aeruginosa promote biofilm formation and persistence in chronic wound infections

This study shows that Pseudomonas aeruginosa proteases cleave SasG in Staphylococcus aureus to induce aggregation and biofilm formation in chronic wound co-infections.

Role of Non-Exponential Reversal times in Aggregation Models of Bacterial Populations

The paper analyzes 1D aggregation models of bacterial populations with non-exponential reversal times, showing they improve aggregate formation and persistence.

Immune Cell Attachment on Material Surface Promotes Bacterial Aggregation and Biofilms

This study shows immune cell attachment on medical device surfaces promotes bacterial biofilm formation, and introduces an antimicrobial composite that converts immune cells into bactericidal effectors.

Pseudomonas aeruginosa faces a fitness trade-off between mucosal colonization and antibiotic tolerance during airway infections

This study investigates how Pseudomonas aeruginosa adapts to the airway environment by balancing biofilm formation for colonization and antibiotic tolerance, revealing trade-offs that contribute to chronic infections.

E. coli cells advance into phase-separated (biofilm-simulating) extracellular polymeric substance containing DNA, HU, and lipopolysaccharide

The paper shows E. coli cells enter phase-separated extracellular polymeric substances containing HU, DNA, and LPS, with LPS modulating liquidity and structural integrity of biofilms.

Loss of O-specific antigen shapes Pseudomonas aeruginosa population microbiogeography in murine preclinical pulmonary infection model

The paper investigates how O-specific antigen loss in Pseudomonas aeruginosa affects population dynamics and aggregate formation in a murine infection model.

Virulence-linked adhesin drives mutualist colonization of the bee gut via biofilm formation

This study identifies biofilm-forming adhesins in Snodgrassella alvi that are essential for its mutualistic colonization of the bee gut.

An Investigation of Cellulase and Epigallocatechin-3-gallate as Enhancers of Antibiotic Efficacy in Polymicrobial Biofilm Treatments

This study investigates cellulase and epigallocatechin-3-gallate as potential enhancers of antibiotic efficacy against polymicrobial biofilms using in vitro assays.