Bacterial biofilms and quorum sensing

Biophysical properties and phenotypes of cell clusters detached from Staphylococcus epidermidis biofilms after matrix-targeted disruption

This study quantifies the physical properties and phenotypes of S. epidermidis biofilm-detached cell clusters after matrix-targeted disruption, revealing differences in cluster morphology and abundance across disruption methods and strains.

Inorganic polyphosphate and pyoverdine synthesis are essential for the virulence of Pseudomonas aeruginosa PAO1 in zebrafish larvae

The paper shows that polyphosphate synthesis and pyoverdine production are critical for Pseudomonas aeruginosa virulence in zebrafish larvae, using a simple infection model.

Biophysical Constraints Dictate the Stability of Social Traits in Pseudomonas aeruginosa Aggregates

This study shows that cell surface hydrophobicity drives micron-scale segregation in Pseudomonas aeruginosa aggregates, physically suppressing cheater fitness and stabilizing cooperation.

Development of a continuous bioreactor to maintain stable nasal microbiomes from swab specimens and synthetic communities

This paper develops a continuous bioreactor to maintain stable nasal microbiomes from swab specimens and synthetic communities, showing long-term stability and S. aureus decolonization.

Microscale spatial fragmentation promotes bacterial survival under antibiotics

The paper shows that microscale spatial fragmentation enhances bacterial survival under antibiotics through physical mechanisms, not genetic resistance.

Acinetobacter spp. with lower susceptibility to quaternary ammonium compounds enriched in microbial communities of frequently used sinks

The paper examines sink drain biofilm microbiomes in academic buildings, finding spatial and temporal variation, dominance of Acinetobacter, and prevalence of QAC resistance genes.

Programmable bacterial adhesion to plastic surfaces for enhanced biodegradation

This paper demonstrates programmable bacterial adhesion to plastic surfaces using curli and Ag43 overexpression, enhancing PET degradation through concurrent enzyme secretion.

The interplay between temperature and growth phase shapes the transcriptional landscape of Pseudomonas aeruginosa

This paper investigates how temperature and growth phase influence the transcriptome of Pseudomonas aeruginosa PAO1, identifying temperature- and phase-specific gene regulation and LasR-regulated genes at different temperatures.

Vancomycin tolerance and dispersion of dual species biofilms of Clostridioides difficile and Vancomycin-resistant Enterococcus faecium

This study investigates how VRE affects C. difficile biofilm formation and vancomycin tolerance in dual-species cultures, finding that VRE inhibits biofilm formation with fermentable glucose but not vancomycin tolerance or dispersion.

Gelatinase regulates the egress of intracellular replicating populations during Enterococcus faecalis infection

This study identifies gelatinase as a key factor enabling Enterococcus faecalis to transition from intracellular replication to extracellular egress during infection.

Chiral Histidine-Modified Gold Nanoclusters Loaded into Cationic Lipid Nanoparticles for Treatment of Biofilm-associated Infections

This paper develops chiral histidine-modified gold nanoclusters encapsulated in cationic lipid nanoparticles to treat biofilm-associated infections, showing enhanced efficacy in reducing bacterial burden and biomass.

AI-2 Production in Fusobacterium nucleatum Is Subspecies-Specific and Uncoupled from Quorum Sensing

This study shows AI-2 production in Fusobacterium nucleatum is subspecies-specific and not linked to quorum sensing.

Analysis of Biofilm Complexity in 3D (ABC3D): An open-source framework for quantitative fractal, textural, and statistical analysis of colony biofilm morphology in three dimensions

ABC3D quantifies 3D biofilm morphology using fractal, textural, and statistical features, showing nutrient availability and cell shape influence biofilm structure in E. coli.

Identification of Antibiofilm Agents against Salmonella enterica from the Pathogen Box Compound Library

This study screens a compound library to identify anti-biofilm agents against Salmonella enterica, finding several compounds that inhibit biofilm formation without significantly affecting growth.

Discovery of inhibitors of the Pseudomonas aeruginosa NADH:ubiquinone oxidoreductase (NQR) that hinder virulence factors

The paper identifies inhibitors of Pseudomonas aeruginosa NQR that affect virulence factors by disrupting electron transfer and proton pumping.

Protozoan predation selects for key symbiotic traits in an environmentally transmitted beneficial symbiosis

This study shows how protozoan grazing pressures shape biofilm traits in Vibrio fischeri, affecting their ability to colonize squid hosts.

Metabolic exchange and siderophore sharing underlie emergent biofilm synergism

The paper identifies metabolic exchange and siderophore sharing mechanisms that enhance biofilm formation among three rhizosphere bacterial species and improve plant growth in iron-depleted soil.

Conformations and sequence determinants in the lipid binding of an adhesive peptide derived from Vibrio cholerae biofilm

The paper investigates how a Vibrio cholerae peptide binds lipids through a β-hairpin conformation, contributing to biofilm adhesion and host colonization.

Regulatory Rewiring Drives Intraspecies Competition in Bacillus subtilis

This study shows that disrupting quorum-sensing pathways in Bacillus subtilis provides a competitive advantage by increasing growth rate, with such mutations found in ~16% of isolates.

Defining the role of Pseudomonas aeruginosa PilY1 in signaling and virulence

This study identifies PilY1-controlled genes and shows PilY1 regulates cAMP and the AlgZ/R system in Pseudomonas aeruginosa, impacting virulence without affecting bacterial survival in key organs.

Identification of Pseudomonas aeruginosa genetic determinants that connect redox metabolism to alginate biosynthesis

This paper identifies genetic determinants in Pseudomonas aeruginosa linking redox metabolism to alginate biosynthesis by analyzing RNA sequencing data and mutant strains.

Opposing range-dependent interactions create complex spatial patterns of antibiotic tolerance in multispecies biofilms

The paper shows that opposing range-dependent interactions between microbial exoproducts create complex spatial patterns of antibiotic tolerance in biofilms.

Furin Inhibition Protects Against Acute Lung Injury in a Mouse Model of Pseudomonas Aeruginosa Infection

This study shows that furin inhibition with BOS-318 reduces acute lung injury and improves survival in mouse models of Pseudomonas aeruginosa infection.

Impact of a DNase I and Vancomycin Drug Combination against a Mature Methicillin Resistant Staphylococcus aureus (MRSA) in vitro Biofilm

This study tests the effectiveness of combining DNase I with vancomycin against mature MRSA biofilms, finding that the combination improves antibiotic efficacy but requires high concentrations for complete eradication.

Discovery of a Human Metabolite that Mimics the Bacterial Quorum-Sensing Autoinducer AI-2

This study identifies L-xylosone as a human metabolite that mimics the bacterial quorum-sensing autoinducer AI-2, revealing a new host-microbe interaction.

DSF signalling integrates c-di-GMP and σ54 pathways with metabolic reprogramming to control Stenotrophomonas maltophilia pathogenicity and antibiotic resistance

This study shows how DSF signaling in S. maltophilia links quorum sensing, c-di-GMP, σ54 pathways, and metabolic reprogramming to control pathogenicity and antibiotic resistance.

Previously uncharacterized aliphatic amino acid positions modulate the apparent catalytic activity of the EAL domain of ZMO_1055 and other cyclic di-GMP specific EAL phosphodiesterases

The paper identifies an aliphatic amino acid substitution that modulates the catalytic activity of the EAL domain in ZMO1055, affecting cyclic di-GMP signaling in Zymomonas mobilis.

The Pseudomonas aeruginosa PrrF sRNAs promote biofilm formation at body temperature

This paper shows that PrrF sRNAs are required for optimal Pseudomonas aeruginosa biofilm formation at 37°C but not 25°C, highlighting temperature's role in biofilm physiology.

Tracking eco-evolutionary dynamics among lung pathobiome members from children with cystic fibrosis

The paper tracks eco-evolutionary dynamics in lung pathobiomes of children with cystic fibrosis, finding that P. aeruginosa variants reduce antagonism towards other pathogens, potentially stabilizing communities.

Transcriptomic dissection of genetically diverse Wrinkly Spreader mutants reveals hidden layers of complexity

The paper uses RNA-seq to analyze 14 WS mutants of Pseudomonas fluorescens SBW25, revealing genotype-specific transcriptional responses and highlighting the polygenic nature of the WS phenotype.