CRISPR and Genetic Engineering

This study shows that genome editing can transfer mitochondrial DNA into the nuclear genome, and provides a solution using TREX1 or TREX2 exonucleases.

The paper describes a gene drive system called RIDD that suppresses Drosophila populations by inducing sterility in females through a dominant sterile homing drive targeting doublesex.

The paper identifies new germline Cas9 promoters in Drosophila that improve drive conversion efficiency while reducing embryo resistance and somatic expression.

The paper introduces CRIMP, a CRISPR/Cas9 insertional mutagenesis system, and CRIMPkit, a plasmid toolkit for efficient gene disruption in fish without genetic compensation.

The paper reports the structure of AsCas12f1 and engineers it to enhance genome editing activity in human cells.

This paper engineers a TnpB-ωRNA system for efficient gene editing in mice, showing improved activity over Cas12f1 and successful disease prevention in a tyrosinaemia model.

This study analyzes DNA double-strand break repair pathways in CRISPR-mediated gene knock-in, finding that multiple repair mechanisms affect precision and efficiency.

The paper describes a CRISPR/Cas9-based curriculum enabling secondary school students to generate LexA lines from GAL4 lines, resulting in 16 novel lines and a standardized workflow.

The paper introduces a CRISPR/Cas9 HDR plasmid platform for Drosophila with higher efficiency than existing methods, enabling new genetic experiments.

This paper expands genetic manipulation tools in Candida species using CRISPR-Cas9 RNP with new drug resistance cassettes, enabling efficient gene deletions and epitope tagging in multiple fungal pathogens.

The paper identifies widespread RNA-guided nucleases in eukaryotes and their viruses, showing they can be used for genome editing in human cells.

The paper introduces CrAAVe-seq, an AAV-based CRISPR screening platform for in vivo cell type-specific analysis, demonstrating its scalability and sensitivity in identifying neuron-essential genes.

This paper develops a CRISPR-based genetic sterilization system for Anopheles gambiae males, achieving high infertility rates and population suppression in competition cages.

This study demonstrates CRISPR-based gene drives in Culex quinquefasciatus, showing biased inheritance of transgenes with modest efficiency.

The paper shows that NbaAgo forms a complex with an effector nuclease to cleave DNA/RNA upon target recognition, providing bacterial immunity against invaders.

The paper identifies compact CRISPR-based activators with durable gene activation, improving upon existing tools in potency and persistence.

SPARCS enables genome-wide CRISPR screening for spatial cellular phenotypes by combining laser microdissection with deep learning, identifying key genes involved in autophagosome formation.

This study identifies CRISPR-induced genomic events like crossover, deletions, and chromothripsis-like rearrangements in plants using a LOH assay.

This study uses prime editing in mice brains to introduce a mutation in the Adrb1 gene, leading to increased activity and exploratory behavior.

CRISPRδ uses dCas13 to specifically block translation without affecting mRNA stability, achieving high gene knockdown specificity in mammalian cells.

This study identifies how directed-evolution mutations in ABE8e enhance DNA-binding affinity and protein stability, improving its base editing activity.

The paper designs and optimizes a synthetic gene regulatory network using dCas12a, achieving higher reporter expression than dCas9-based systems.

This study removes a genomic duplication in a mouse model using DN-CRISPRs, improving synaptic transmission and behavior.

The paper investigates DNA transposition activity of PGBD5 in human cells, showing that overexpression causes DNA damage and challenges previous findings of transposition.

This paper engineers Nme2Cas9 base editors to improve activity and targeting scope, enabling correction of MECP2 mutations with minimal off-target effects.

The paper introduces a PCR-RFLP method called PIM-RFLP to efficiently detect CRISPR-induced indels in cell clones and pools.

This study applies DIPA-CRISPR to Aedes aegypti, demonstrating its effectiveness in polytrophic meroistic ovaries and confirming optimal injection timing for gene editing.

This study demonstrates the use of protecting oligos to enhance crRNA stability and potency for in vivo genome editing via AAV co-delivery in mice.

The paper describes the construction of CRISPR/Cas9 vectors for knocking out SSSII genes using Gateway cloning technology.

The paper demonstrates efficient multiplexed gene editing in immune cells using Cas12a/Cpf1 knock-in mice and associated viral vectors.