Trypanosoma species research and implications

The paper identifies critical domains of KREPA3 essential for RNA editing complex structure and function in Trypanosoma brucei, showing stage-specific effects of mutations.

This study identifies and characterizes non-histone protein methylation in Trypanosoma cruzi using mass spectrometry-based proteomics, revealing its role in various biological processes.

The paper uses AlphaFold2 and genetic approaches to identify protein complexes in trypanosomatids, revealing structurally conserved and novel complexes involving MRG domain-containing proteins.

The paper reports the cryo-EM structure of the RESC1-RESC2 complex from Trypanosoma brucei, revealing its role in guide RNA selection through 5-triphosphate binding.

This study uses long-read direct RNA sequencing to identify 207 novel lncRNAs in T. brucei, including stage-specific ones, providing a comprehensive view of its transcriptome.

This study shows that eIF3m gene silencing in Rhodnius prolixus disrupts blood meal digestion, moulting, reproduction, and survival, highlighting its critical role in triatomine physiology.

This study identifies heme-deficient metabolism and impaired cellular differentiation as evolutionary trade-offs for human infectivity in Trypanosoma brucei gambiense due to HpHbR inactivation.

The paper investigates the roles of RESC13 and RESC14 in kinetoplastid RNA editing across multiple transcripts, finding conserved and transcript-specific functions.

The paper investigates the roles of EIF4E2, EIF4E5, and EIF4E6 in Trypanosoma brucei, finding that EIF4E2 maintains S-phase mRNA abundance and is involved in mRNA stabilization.

The paper describes DNA-cholesterol nanoparticles that kill Trypanosoma brucei by disrupting lysosomal membrane integrity and demonstrate a dual-target approach using DNAzymes.

The paper investigates dynamic changes in Bdf3 chromatin occupancy during Trypanosoma brucei differentiation from bloodstream to insect stage, finding increased occupancy at genes involved in surface protein remodeling.

This paper sequences and compares the genomes of two non-pathogenic trypanosomes to identify genomic features associated with host and vector specificity.

The paper identifies multiple regulatory sequences in the 3'-untranslated regions of mRNAs that control bloodstream-form-specific gene expression in Trypanosoma brucei.

The paper uses single-cell RNA sequencing to identify and characterize sexual stages in Trypanosoma brucei, revealing gene expression patterns associated with gamete formation.

This study shows that oxidative phosphorylation is essential for Trypanosoma brucei migration within tsetse flies but not for initial colonization.

The paper develops a high-content screen to identify inhibitors of mitochondrial DNA maintenance in trypanosomes and identifies a novel kDNA-targeting compound.

The paper shows that the SLS pathway in Trypanosoma brucei is triggered by disruptions in ER, Golgi, or mitochondrial protein handling and identifies PK3 phosphorylation as critical for its activation.

This study investigates the role of the EIF4E1-4EIP cap-binding complex in translation repression in Trypanosoma brucei, showing that 4EIP stabilizes EIF4E1 and recruits TUT3, but NOT complex may be involved in 4EIP function.

The paper investigates how the position of DNA double strand breaks influences gene expression and VSG switching pathway choice in Trypanosoma brucei.

This study identifies the roles of histone variants H3v and H4v and base J in transcription termination, antigenic variation, and proliferation in Trypanosoma brucei.

This study uses scRNA-seq to analyze Trypanosoma brucei development in tsetse fly salivary glands, revealing dynamic VSG gene activation and monoallelic expression establishment.

The paper describes two new Trypanosoma brucei brucei strains with minimal in vitro passage and reports gene copy-number changes associated with culture adaptation.

The paper sequences maxicircles from T. cruzi strains using long-read sequencing, revealing their architecture, evolutionary relationships, and proposing a new nomenclature.

The paper re-analyzes genomic data to define clades of monomorphic Trypanozoon species and identifies lineage-specific variation, challenging traditional classifications and suggesting evolutionary relationships.

The paper presents an improved genome assembly of Trypanosoma cruzi and reveals a unique genomic structure involving subtelomeric regions and retrotransposons contributing to antigenic variation.

The paper identifies JBP3, a J-binding protein in Leishmania, which associates with complexes involved in transcription termination and chromatin remodeling, enabling polycistronic transcription and post-transcriptional regulation.

The paper identifies a novel mechanism in trypanosomes where inter-chromosomal interactions between an antigen coding gene and an mRNA splicing locus ensure monogenic antigen expression.

The paper identifies tsetse fly blood-meal sources, endosymbiont infections, and trypanosome infections in the Maasai Mara National Reserve, finding that hippopotamus and buffalo are significant hosts and that Sodalis glossinidius endosymbionts correlate with higher trypanosome infection rates.

The paper develops a high-resolution melt assay to identify Trypanosoma brucei gambiense and T. b. rhodesiense in tsetse flies, offering a rapid and specific alternative to multiple PCR methods.