Trypanosoma species research and implications

Proteomic mapping of novel tubulin post-translational modifications in Trypanosoma cruzi cytoskeleton

The paper identifies and maps novel tubulin post-translational modifications in Trypanosoma cruzi using proteomic analysis.

Anatomy and mechanics of tsetse fly blood feeding

This study examines the anatomy and mechanics of tsetse fly blood feeding, finding that efficient feeding is achieved through subtle adaptations rather than unique structures or forces.

The modular origin and evolutionary expansion of the enigmatic DGF-1 protein family in trypanosomatids

The paper re-annotates and characterizes the DGF-1 gene family in T. cruzi, revealing its modular structure and evolutionary history across trypanosomatids.

Chemotactic responses of Trypanosoma brucei procyclic forms to proline and other metabolites

This study shows Trypanosoma brucei procyclic forms respond to metabolites like proline and succinate through chemotaxis, involving cAMP signaling and specific proteins.

A luminal proteome of the endoplasmic reticulum and Golgi apparatus reveals a novel modulator of ER stress tolerance in African trypanosomes

This study maps the luminal proteome of the ER and Golgi in T. brucei using TurboID proximity biotinylation, identifying TbMANF as a novel modulator of ER stress tolerance in African trypanosomes.

BDF6 deficiency severely compromises intracellular amastigotes development and infectivity in Trypanosoma cruzi

This study shows that BDF6 deficiency impairs intracellular development and infectivity of Trypanosoma cruzi parasites.

Vampire bats in Belize harbor multiple Trypanosoma cruzi genotypes: implications for parasite transmission at the wildlife-domestic-human interface

The paper identifies multiple Trypanosoma cruzi genotypes in vampire bats in Belize, highlighting their potential role in linking sylvatic, domestic, and human transmission cycles of Chagas disease.

Functional Mapping of the Trypanosoma cruzi Serinome by Fluorophosphonate Activity-Based Protein Profiling

This study maps the serinome of Trypanosoma cruzi using fluorophosphonate activity-based protein profiling, identifying 37 SH-like proteins and their functional roles.

De novo assembly of the Trypanosoma congolense genome reveals an organisation influenced by antigenic variation but distinct from Trypanosoma brucei

This paper presents a telomere-telomere genome assembly of Trypanosoma congolense, revealing distinct VSG organization and expression mechanisms compared to Trypanosoma brucei.

Creating complete life histories of individual female tsetse (Glossina spp) to study the effects of meteorological conditions on fly size in Zimbabwe

The paper estimates life histories of tsetse flies to model how meteorological conditions affect their size, finding that egg and wing lengths are influenced by NDVI and temperature.

The major diagnostic VSG LiTat 1.3 of the human parasite Trypanosoma brucei gambiense is a trimer in solution

The paper shows that LiTat 1.3, a key VSG for diagnosing T. b. gambiense, forms homotrimers in solution, not homodimers, using structural and biophysical methods.

Structural basis for the inhibition of Trypanosoma brucei enolase by a camelid single-domain antibody

This paper identifies a camelid single-domain antibody that inhibits T. brucei enolase and elucidates its inhibitory mechanism using biochemical, biophysical, and structural methods.

Renal and endothelial biomarkers in Chagas disease in the Brazilian Amazon region: early indicators of kidney injury and disease progression

This study identifies elevated biomarkers in blood and urine that indicate early renal and endothelial dysfunction in Chagas disease patients in the Brazilian Amazon, undetectable by conventional tests.

Glycerol metabolism triggers trypanosome differentiation into transmissible forms in mammalian tissue-like conditions

This study identifies glycerol as a trigger for Trypanosoma brucei differentiation into transmissible forms under tissue-like conditions, challenging the traditional bloodstream model of transmission.

Acute Sublethal Heat Stress Impairs Blood Feeding and Trypanosome Infection in the Kissing Bug, Rhodnius prolixus

This study shows that acute heat stress reduces blood feeding and Trypanosome infection in Rhodnius prolixus, with recovery improving these outcomes.

Cyclic AMP compartmentalization drives signal specificity to control vector colonization and mammalian host infection by American trypanosomes

This study identifies two cAMP microdomains in Trypanosoma cruzi, showing their distinct roles in parasite survival and infection.

The bromodomain protein Bdf3 is sufficient to activate expression of a procyclin gene in bloodstream stage African trypanosomes

This paper shows that Bdf3 activation at the EP promoter in bloodstream Trypanosoma brucei increases EP1 transcript levels, supporting its role in initiating transcription during differentiation to the procyclic stage.

Integrative genomics identifies candidate genes underlying trypanotolerance in hybrid African cattle

This study identifies candidate genes for trypanotolerance in African cattle by integrating genomic and transcriptomic data from hybrid populations.

Remodeling of tRNA modification in Trypanosoma cruzi life forms

This study profiles tRNA modifications in different life stages of Trypanosoma cruzi and finds that hydroxywybutosine modification levels regulate metacyclogenesis.

The Trypanosoma cruzi cell atlas; a single-cell resource for understanding parasite population heterogeneity and differentiation.

This paper creates a single-cell atlas of Trypanosoma cruzi, revealing life cycle genes, cell heterogeneity, and transcriptomic changes during differentiation.

Cell surface localisation of GPI-anchored receptors in Trypanosoma brucei

This study shows GPI-anchored receptors in Trypanosoma brucei are localized across the entire cell surface, not just the flagellar pocket, challenging previous assumptions about immune evasion.

Loss of Vitellogenin Receptor Function Results in Yolk Depletion, Virome Expansion and Reduced Bacterial Load Within the Oocytes of Rhodnius prolixus

This paper shows that blocking the vitellogenin receptor in Rhodnius prolixus causes yolk depletion and changes in the oocyte microbiome, including reduced bacteria and increased viruses.

A Genome-Wide Genetic Screen Identifies a Novel kDNA Replication Protein in Trypanosomes

This study uses a genetic screen in Trypanosoma brucei to identify novel kDNA maintenance factors, validates one as a novel replication factor, and provides a prioritized list of candidates.

Revisiting Cysteine Protease Function in Trypanosoma cruzi: Implications for Parasite Egress and Differentiation

This study investigates the role of cysteine proteases in Trypanosoma cruzi differentiation and egress, showing that their inhibition blocks maturation and delays parasite release.

Single-cell RNA-seq reveals trans-sialidase-like superfamily gene expression heterogeneity in Trypanosoma cruzi populations

This study uses single-cell RNA sequencing to show that T. cruzi trypomastigote cells express trans-sialidase-like superfamily genes with high heterogeneity and no recurrent combinations.

Specialised RNA decay fine-tunes monogenic antigen expression in African trypanosomes

The paper identifies three proteins that negatively regulate ESAG transcripts through specialised RNA decay in Trypanosoma brucei, revealing a novel post-transcriptional regulatory mechanism.

Germline Targeted Baboon Apolipoprotein L-1 Protects Mice Against African Trypanosomes

This study generates trypanosome-resistant murine models expressing primate APOL1 proteins to investigate their role in immunity, finding that baboon APOL1 provides partial protection but with limited expression, while a chimeric APOL1 variant shows protection with a fitness cost.

A Trypanosome Trifecta: an independently tunable triple inducer system for genetic studies in Trypanosoma brucei.

This paper develops a triple inducer system in Trypanosoma brucei for independently regulating three genetic elements, demonstrating independent control and tunability.

The trypanosome mRNA decapping enzyme ALPH1 prefers caps without m7G methylation and produces diphosphate-RNA

This study characterizes the trypanosome mRNA decapping enzyme ALPH1, showing it preferentially cleaves non-m7G-capped RNAs and produces diphosphate-RNA.

Glycosomal phosphoenolpyruvate carboxykinase CRISPR/Cas9-deletion and its role in Trypanosoma cruzi metacyclogenesis and infectivity in mammalian host

This study uses CRISPR/Cas9 to delete PEPCK in T. cruzi, showing it impairs differentiation and host invasion but enhances replication once inside host cells.