Circular RNAs in diseases

Distinct virus-derived circular RNA molecule influences host response during SARS-CoV-2 infection

The paper identifies a novel SARS-CoV-2-derived circular RNA, circ7b8N, which modulates host gene expression and is detectable in clinical samples, suggesting a role in host-pathogen interactions.

The RNase and RNA binding activities of selected RNase R truncations and mutations plus a detailed step by step protocol to purify recombinant RNase R

This paper presents a cost-effective protocol to purify recombinant RNase R and evaluates mutations affecting its RNA binding and degradation activities.

Detection of Candidate Circular RNAs to Monitor Anti-Hormonal Response in the Mammary Gland

The paper identifies circular RNAs that change in response to anti-hormonal treatments in mouse models, suggesting potential as non-invasive biomarkers for therapeutic response.

Benchmarking circRNA Detection Tools from Long-Read Sequencing Using Data-Driven and Flexible Simulation Framework

This paper benchmarks circRNA detection tools using a simulation framework and finds they vary in performance, with limited overlap in detected circRNAs and significant computational efficiency differences.

The mechanism of Hsa_circ_0000629 in bronchial asthma through sponge adsorption of miR-212-5p/NLRP3

This study investigates how Hsa_circ_0000629 regulates bronchial asthma by sponging miR-212-5p to modulate NLRP3 expression, leading to reduced inflammation and apoptosis.

A dual-function variant on chromosome 17 regulates circRNA expression and splicing in multiple sclerosis

The paper identifies genetic variants that regulate circRNA expression and splicing in multiple sclerosis, highlighting a dual-function variant on chromosome 17.

Assessing the impact of parental linear gene normalization on the performance of statistical models for circular RNA differential expression analysis

This study evaluates normalization and filtering strategies for circRNA differential expression analysis, finding that edgeRs filterByExpr() and linear-aware normalization improve performance.

Mechanism of circZNF827-mediated transcriptional repression during neuronal differentiation

This study investigates how circZNF827 and hnRNPL repress transcription during neuronal differentiation by analyzing transcriptomic and epigenetic changes.

Circular RNA identification using a genomic language model and a small number of authenticated examples

The paper introduces circFormer, a genomic language model-driven method for circular RNA identification, which outperforms traditional approaches and validates a high percentage of its candidates.

Circular Smad1-Encoded Polypeptide Regulates Myogenesis

This study identifies circSmad1-194aa as a functional peptide that promotes myogenesis by regulating Id1 and Myod1 expression in mouse skeletal muscle cells.

Global Mapping of circRNA-Target RNA Interactions Reveals P-Body-Mediated Translational Repression

The paper maps circRNA-target RNA interactions using RIC-seq, identifies translational repression by P-bodies, and highlights disease relevance of interaction sites.

Mitochondrial CircRNA CircMT-RNR2 Safeguards Antioxidant Defense to Support Fibroblast Functions in Wound Repair

CircMT-RNR2 promotes fibroblast function and maintains mitochondrial redox balance via stabilization of PRDX3, offering a therapeutic target for diabetic foot ulcers.

Common Pitfalls in CircRNA Detection and Quantification

This study highlights issues with circRNA detection in poly(A)-enriched RNA-seq data, showing it leads to false positives and recommends total RNA-seq with rRNA depletion for accurate profiling.

circPCMTD1: A protein-coding circular RNA that regulates DNA damage response in BCR/ABL-positive leukemias

This study identifies circPCMTD1 as a circular RNA that promotes leukemic cell proliferation by enhancing DNA damage response tolerance through a novel peptide product.

The RNA-binding protein NOVA-1 regulates circRNA expression, alternative splicing, and aging in C. elegans

This study shows that NOVA-1 regulates circRNA expression, alternative splicing, and aging in C. elegans by modulating splicing events and circRNA production.

HIV-1 virions selectively package circESYT2 to sculpt an actin scaffold that constraints egress

This study identifies circESYT2 as a host circular RNA selectively packaged by HIV-1 to modulate actin dynamics and viral egress.

Human left ventricle circRNA-miRNA-mRNA network analyses reveals a novel proangiogenic role for circNPHP1 under ischemic conditions

This study identifies a circRNA-miRNA-mRNA network in ischemic heart disease, revealing circNPHP1's role in enhancing angiogenesis by sponging miR-221-3p to upregulate VEGF-A and BCL2.

Comparative analysis of eccDNA and circRNA tools shows increased accuracy of tool combination

The paper evaluates circular nucleic acid detection tools, finding that combining at least three tools improves accuracy.

Circular RNA profiling revealed an evolutionarily conserved circACACA promotes liver lipid metabolism, oxidative stress, and autophagy disorder in a ceRNA manner

This study identifies circACACA as an evolutionarily conserved circRNA that disrupts lipid metabolism and induces oxidative stress and autophagy disorder in NAFLD through a ceRNA mechanism.

3' to 5' Translation of Circular RNAs?

The paper presents evidence that eukaryotic circular RNAs can be translated in the 3' to 5' direction, producing novel proteins with distinct features, and identifies millions of potential BT proteins across species.

circASbase：A Comprehensive Database of Alternative Splicing Events in circRNAs

circASbase is a database cataloging 452,129 alternative splicing events in 884,047 circRNAs from 581 samples across 13 species, revealing unique splicing features and functional impacts of circRNAs.

Accurate Reconstruction of Circular RNAs from Complex Rolling Circular Long Reads with CircPlex

The paper introduces CircPlex, a method to accurately reconstruct circRNAs from complex rolling circular long reads by addressing repeat pattern complexities, improving detection sensitivity and uncovering novel isoforms.

Scalable production of immune-silent circular RNAs for efficient protein expression

This paper develops a scalable method for producing high-purity circular RNAs with minimal immune activation and efficient protein expression.

abcFISH enables multiplexed, single-molecule visualization of circular RNA spatial heterogeneity

abcFISH enables multiplexed, single-molecule imaging of circRNA spatial heterogeneity, revealing cell-type-specific expression and functional interactions.

RNA denaturation underlies circular RNA separation

This study evaluates gel electrophoresis and HPLC-SEC for separating circRNAs, showing both rely on RNA denaturation and that magnesium ions impair separation.

A novel role of circCPSF6 regulating antiviral innate immunity via miR-665 and PCBP2-IPS-1 axis

This study identifies circCPSF6 as an antiviral factor that regulates host innate immunity by sequestering miR-665 and modulating PCBP2-IPS-1 interactions.

Circular RNA Pde4dip regulates myogenesis by interacting with Zfp143 mRNA: A novel regulatory axis

The paper identifies circPde4dip as an mRNA-interacting circular RNA that regulates myogenesis by modulating Zfp143 expression in C2C12 myoblasts.

Resistance to Radiation Enhances Metastasis by Altering RNA Metabolism

This study shows that radiation resistance in TNBC enhances metastasis through RNA metabolism changes, specifically via HNRNPL-mediated circRNAs that regulate ITGβ3 expression.

Identification of Circular RNAs associated with Ageing of the Dorsolateral Prefrontal Cortex across the Adult Lifespan

The paper identifies seven age-associated circRNAs in the dorsolateral prefrontal cortex across the adult lifespan, linked to synapse regulation and post-transcriptional networks.

Deciphering the circular RNAs landscape in amyotrophic lateral sclerosis

This study identifies circRNAs involved in ALS and related motor neuron diseases, showing their regulatory roles through miRNA sponging and interactions with RNA-binding proteins.