Per- and polyfluoroalkyl substances research

Single-cell RNA sequencing uncovers cell-type-specific reprogramming of water-channel and cell-wall genes in PFOA uptake by lettuce root tips

The paper uses single-cell RNA sequencing to identify cell-type-specific gene expression changes in lettuce root tips exposed to PFOA, revealing mechanisms of uptake and deposition.

Short-chain PFAS exposure alters embryonic development and behavior in zebrafish

This study shows that early-life TFA exposure in zebrafish embryos alters development and increases anxiety-like behaviors, with associated proteomic changes.

Transcriptomic insights into developmental toxicity of per- and poly-fluoroalkyl substances (PFAS) in Caenorhabditis elegans: The potential key role of xenobiotic detoxification pathway

This study investigates the developmental toxicity of PFAS in C. elegans, finding that PFDA and PFOS disrupt development and activate xenobiotic detoxification pathways.

A Human Next Generation PBK Model for PFOA

This paper develops a mechanistic NG-PBK model for PFOA using in vitro and in silico data, showing it accurately predicts toxicokinetics and can be extrapolated to other PFAS for risk assessment.

Exposure to per- and polyfluoroalkyl substances elicits cell type-specific impacts on p53 and TGF-β signaling pathways

This study examines the cell type-specific effects of PFAS compounds on p53 and TGF-β signaling pathways in human cell lines.

PFOA induced metabolic and immune perturbations in a SARS-2 infection model

This study examines how PFOA exposure affects metabolism and immune response to SARS-2 in ferrets, finding altered metabolic responses and impaired immune function.

Structural insights into PFAS-β-lactoglobulin binding mechanism mediating PFAS toxicity

This study reveals how β-lactoglobulin binds PFAS compounds through structural analysis and molecular dynamics, showing hydrophobic and electrostatic interactions that stabilize the binding.

Differential Toxicity of Perfluorooctane Sulfonate (PFOS) in Wild-Type and Oatp1d1 Mutant Zebrafish Embryos

This study compares PFOS toxicity in wild-type and oatp1d1 mutant zebrafish embryos, finding higher susceptibility in mutants with altered gene expression and developmental abnormalities.

Maternal PFAS Transfer through Lactation: Dolphin Milk Reveals Routes of Early-Life Exposure

This study analyzes PFAS concentrations in dolphin milk over two years, finding high levels of PFOS exceeding safety thresholds and suggesting breastmilk as a major source of early-life PFAS exposure.

Identification of Functional Genetic Components Modulating Toxicity Response to PFOS using Genome-wide CRISPR Screens in HepG2/C3A cells

This study identifies 340 genes modulating PFOS-induced cytotoxicity in liver cells using CRISPR screens and validates two genes, SLC6A9 and CPSF2, with functional and molecular evidence.

Biotransformation and biodefluorination of chlorinated polyfluorocarboxylic acids by Acetobacterium species

This study investigates the biodefluorination of chlorinated polyfluorocarboxylic acids by Acetobacterium species, identifying defluorination pathways and enzyme requirements.

Early exposure to PFAS disrupts neuro-muscular development in zebrafish embryos

This study investigates how PFOA exposure disrupts neuro-muscular development in zebrafish embryos, revealing molecular perturbations linked to behavioral changes.

Genome-wide CRISPR screens identified C18orf32 as a novel regulator of lipid metabolism that mediates PFOA-induced toxicity

The paper identifies C18orf32 as a novel regulator of lipid metabolism involved in PFOA-induced toxicity through CRISPR screening and transcriptomic analysis.

Comparative Transcriptomic Analysis of Perfluoroalkyl Substances-Induced Responses of Exponential and Stationary Phase Escherichia coli

This study uses RNA sequencing to compare the transcriptional responses of Escherichia coli to PFAS and non-fluorinated compounds across growth phases, revealing distinct gene expression changes induced by PFAS.

Structural and Biophysical Basis for PFAS Binding by Human Sterol Carrier Protein-2

The paper investigates SCP2's interaction with PFAS, showing it binds PFAS for the first time and identifies residues involved in this interaction.

Metabolic effects and biotransformation of perfluorohexyloctane (F6H8) in human hepatocytes

This study examines the metabolic effects and biotransformation of F6H8 in human hepatocytes, revealing concentration-dependent metabolic alterations and the formation of a PFAS-like biotransformation product.

Temperature modulates PFAS accumulation and energy allocation in sheepshead minnows

This study shows warming reshapes PFAS tissue concentrations and distribution, influencing fish energy-allocation trade-offs.

Acute PFOS exposure consistently dysregulates glucose-stimulated insulin secretion across model systems

This study shows PFOS exposure dysregulates glucose-stimulated insulin secretion in multiple models, with effects varying by exposure duration and model system.

Synthesis of perfluorooctanoic acid-containing membrane lipids by human pathobionts

This study shows human gut bacteria can synthesize fluoroalkyl-containing membrane lipids in response to PFOA exposure, suggesting new pathways for PFAS bioaccumulation and host-microbe interactions.

Multi-Modal Mass Spectrometry Imaging Reveals Isomer-Specific PFOS Distribution and Spatial Lipid Disruption in Mouse Liver

This study uses multi-modal mass spectrometry imaging to show that PFOS isomers have distinct spatial distributions in mouse liver and cause zonal lipid disruptions.

Brief report on differential toxicity of legacy and second-generation PFAS on dopaminergic neurons and mitochondria

This study compares the neurotoxicity and mitochondrial effects of legacy and second-generation PFAS using C. elegans and rat brain mitochondria, finding distinct mechanisms between the two classes.

Mycothiol Conjugation Drives Biotransformation and Biodefluorination of Fluorotelomer Carboxylic Acids by Actinomycetota and Sludge Microbiomes

This study identifies mycothiol conjugation as a novel mechanism for PFAS biodefluorination by Actinomycetota and sludge microbiomes, resolving mass balance issues and revealing a cascading metabolic network.

Arctic-Atlantic gradient shaped PFAS exposure variability in sympatric guillemot species off Iceland

The paper examines PFAS exposure variability in sympatric guillemot species, revealing structured patterns linked to Arctic-Atlantic oceanographic gradients and foraging strategies.

Inoculation with arbuscular mycorrhizal fungi can increase the concentration of PFAS compounds in cereal crops

This study shows that arbuscular mycorrhizal fungi can increase PFAS concentrations in cereal crops, depending on soil contamination and plant species.

PFAS Compounds PFOA and Gen X are Teratogenic to Sea Urchin Embryos

This study shows that both PFOA and Gen X, two PFAS compounds, are teratogenic to sea urchin embryos, with Gen X causing more severe developmental effects.

Updated Guidance for Communicating PFAS Identification Confidence with Ion Mobility Spectrometry

An audit of 38 PFAS non-targeted studies from 2023 found confidence levels often missing or misapplied; a five-level, sublevel-free checklist with predefined platform tolerances is proposed for ion mobility workflows.

Developmental Toxicity and Lethality of Structurally Diverse PFAS in Zebrafish

This study evaluates the lethality and developmental toxicity of 17 structurally diverse PFAS in zebrafish under pH-neutral conditions, finding that chain length and head group type significantly influence toxicity.

Mobilization of genes encoding potential PFAS-degradation enzymes and positive selection in cyanobacteria

The paper identifies mobilized genes for PFAS-degradation enzymes in cyanobacteria and suggests positive selection driven by stress mitigation rather than metabolic benefit.

Chronic exposure to PFAS triggers systems-level cellular reprogramming independent of their bioaccumulation

This study shows chronic PFAS exposure causes cellular reprogramming through oxidative stress and lipid metabolism changes, even with minimal accumulation.

Extensive PFAS accumulation by human gut bacteria

This study shows human gut bacteria can accumulate PFAS, with some species like Bacteroides uniformis accumulating them to high concentrations, and identifies mechanisms involving transmembrane transport and efflux pumps.