Sheena E. Radford

Distinct mechanistic pathways of early tauopathy revealed by MAPT mutations

This study uses mouse models with tau mutations to show two distinct pathogenic mechanisms in tauopathies, with different progression patterns and responses to amyloid.

Buffer-dependent conformational dynamics of α-synuclein revealed by nanopipette electrospray ionisation ion mobility mass spectrometry

This study uses nanopipette electrospray ionisation ion mobility mass spectrometry to investigate how buffer conditions affect α-synuclein conformational dynamics.

Methods for high throughput discovery of fluoroprobes that recognize tau fibril polymorphs

The paper develops a high-throughput method to identify fluoroprobes that distinguish between tau fibril polymorphs using paDSF and chemical screening.

Design and Characterisation of Photoactivatable and Lysine Reactive o-Nitrobenzyl Alcohol-Based Crosslinkers

This study develops and characterizes oNBA-based crosslinkers for structural proteomics, showing high labelling efficiency and crosslinking yields with optimized photolysis conditions.

Residues 2-7 of alpha-synuclein regulate amyloid formation via lipid-dependent and -independent pathways

This study identifies residues 2-7 of alpha-synuclein as regulators of amyloid formation, showing they slow fibril formation and reduce aggregation in vitro and in vivo without affecting membrane binding.

Sculpting conducting nanopore size and shape through de novo protein design

This paper designs stable transmembrane beta-barrel nanopores with tailored sizes and shapes using de novo protein design, achieving conductances ranging from 110 pS to 430 pS.

The in-tissue molecular architecture of β-amyloid pathology in the mammalian brain.

This study reveals the in-tissue molecular architecture of β-amyloid fibrils in a mouse model of Alzheimer's disease, showing their arrangement in lattices and their structural differences due to the Arctic mutation.

De novo design of transmembrane beta-barrels

This study successfully designs transmembrane beta-barrels from first principles, revealing structural constraints and enabling custom pore design for biotechnology applications.