snRNA-seq

Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers

The paper uses snRNA-seq to analyze transcriptional diversity in multinucleated skeletal myofibers, revealing distinct myonuclear populations and novel gene markers.

Single cell transcriptome profiling of the human alcohol-dependent brain

This study uses snRNA-seq to analyze transcriptomes of 16,000 nuclei from human prefrontal cortex in alcoholics and controls, identifying cell type-specific gene expression changes associated with alcohol dependence.

Single-nucleus RNA-seq identifies Huntington disease astrocyte states

This study uses snRNA-seq to identify distinct transcriptional states of astrocytes in Huntington disease, revealing gene expression changes compared to controls.

Systematic Comparison of High-throughput Single-Cell and Single-Nucleus Transcriptomes during Cardiomyocyte Differentiation

The paper compares Drop-seq and DroNc-seq in profiling cardiomyocyte differentiation, finding similar cell types and trajectories in both methods.

Transcriptional analysis supports the expression of human snRNA variants and reveals U2 snRNA homeostasis by an abundant U2 variant

The paper identifies transcribed snRNA variants and shows how sequence variation and promoter regulation affect U2 snRNA homeostasis and splicing.

Single-nucleus RNA-Seq reveals a new type of brown adipocyte regulating thermogenesis

This study identifies a novel adipocyte subtype using single-nucleus RNA-Seq that regulates thermogenesis via paracrine signaling in humans and mice.

Iterative Refinement of Cellular Identity from Single-Cell Data Using Online Learning

The paper develops an online learning algorithm for integrating large single-cell datasets, enabling efficient processing of millions of cells with reduced memory usage and allowing iterative refinement of cellular identity.

Single cell and single nucleus RNA-Seq reveal cellular heterogeneity and homeostatic regulatory networks in adult mouse stria vascularis

The paper uses single cell and single nucleus RNA-sequencing to identify cell populations and gene regulatory networks in adult mouse stria vascularis, linking known deafness genes to specific cell types.

A Spatiomolecular Map of the Striatum.

The paper maps the molecular and spatial organization of striatal projection neurons, identifying new subtypes and spatial domains in both mice and non-human primates.

Probing glioblastoma and its microenvironment using single-nucleus and single-cell sequencing

The paper uses single-cell and single-nucleus sequencing to analyze glioblastoma microenvironment composition and interactions, identifying diverse cell types and pathway rewiring.

Topology of the U12-U6atac snRNA complex of the minor spliceosome and binding by NTC-related protein RBM22

This study characterizes the structure of the U12-U6atac snRNA complex in the minor spliceosome and shows RBM22 binds both U2-U6 and U12-U6atac complexes with similar affinity.

Nuclei multiplexing with barcoded antibodies for single-nucleus genomics

The paper introduces a method to multiplex single-nucleus RNA-seq using barcoded antibodies, reducing costs and enabling large-scale studies.

Systematic comparative analysis of single cell RNA-sequencing methods

Seven single-cell and single-nucleus protocols run side by side on the same cell lines, PBMCs and mouse cortex, analysed through one pipeline: 10x Chromium led the high-throughput methods.

The RNA demethylase FTO targets m6Am in snRNA to establish distinct methyl isoforms that influence splicing

This study shows FTO demethylates m2-snRNA to regulate splicing by controlling reversible methylation of snRNAs.

Equivalent high-resolution identification of neuronal cell types with single-nucleus and single-cell RNA-sequencing

The paper shows that single-nucleus and single-cell RNA-seq can equally identify neuronal cell types in mouse visual cortex when intronic sequences are included.

Comparative analysis of kidney organoid and adult human kidney single cell and single nucleus transcriptomes

The paper compares kidney organoid and adult human kidney single-cell and single-nucleus transcriptomes to assess differentiation maturity and identify transcription factor networks.

sNucDrop-Seq: Dissecting cell-type composition and neuronal activity state in mammalian brains by massively parallel single-nucleus RNA-Seq

sNucDrop-Seq isolates nuclei from adult mouse brains to analyze cell-type composition and neuronal activity states at single-nucleus resolution.