Lipid Membrane Structure and Behavior

Independent tuning of outer membrane fluidity and mechanics in Gram-negative bacteria

This study shows that outer membrane fluidity and mechanics in Gram-negative bacteria can be independently controlled by altering lipid composition and crosslinking.

Fluorescence imaging-induced phospholipid oxidation drives membrane phase separation by shifting miscibility boundaries

This study shows fluorescence imaging induces phospholipid oxidation, shifting miscibility boundaries and causing membrane phase separation in model systems.

Domain Dissolution in Supported Lipid Bilayers Triggered by Unsaturated Phospholipid Addition

This study demonstrates that adding unsaturated phospholipid liposomes can dissolve lipid rafts in supported lipid bilayers, altering domain composition and redistributing raft-localized molecules.

Ether linkages in phospholipids provide modular control of membrane mechanics

The paper shows that ether linkages in phospholipids modulate membrane mechanics and topology through distinct physical mechanisms, explaining their evolutionary conservation and biological roles.

Diverse ancestral myosin motors generate and segregate distinct types of nanocluster-rich domains at the plasma membrane

The paper shows that two classes of myosins organize distinct membrane domains through active forces, enabling spatial segregation of nanoclusters and modulating information transduction.

Peptide Sequence Features that Reduce Membrane Pore Line Tension

The paper identifies sequence features of α-helical peptides that reduce membrane line tension through computational simulations and evolutionary optimization.

Large phospholipid-number asymmetry is not required to reproduce plasma membrane physical properties

The paper uses simulations to show that lipid-type asymmetry, not phospholipid-number imbalance, is sufficient to reproduce key physical properties of plasma membranes.

Cell-sized droplet interfaces reorganize protein secondary structures through confinement-enhanced membrane interactions

This study shows that membrane interactions can reorganize denatured proteins into β-sheet structures under confinement, using cell-sized droplets as a platform.

Ups and downs of liquid-liquid transitions in GUV membranes from osmolarity and aspiration tensions

This study investigates how osmotic pressure and micropipette aspiration affect liquid-liquid phase transitions in giant unilamellar vesicles, finding both can increase transition temperatures.

Molecular Mechanism of Lipid Recognition and Membrane-Guided Gating in Plant Minimal START Proteins

This study identifies a bipartite ligand-binding mechanism and membrane-responsive gating in plant minimal START proteins, confirming their role in lipid exchange.

Lipid Composition Modulates Secondary Structure at the Biocondensate-Membrane Interface

The paper uses SEIRAS to show that membrane charge affects the secondary structure of poly-GR condensates at the membrane interface.

Hydrophobic mismatch induces lipid sorting based on tail unsaturation

This paper uses molecular dynamics simulations to show that hydrophobic mismatch between transmembrane proteins and lipid membranes leads to lipid sorting based on tail unsaturation.

Septin Architecture Dictates Size-Dependent Diffusion Barriers on biomimetic Membranes

This study shows septins create size-dependent diffusion barriers on synthetic membranes by organizing into filaments, restricting the movement of membrane-bound objects with large cytosolic domains.

On the effect of applied lateral tension on the deformation energetics of biological membranes and the lipid dynamics within

MARTINI 2.2 simulations show lateral tension changes the free energies of membrane bending and thickness deformations through a projected-area term (γΔΔA), while leaving lipid mixing, residence and exchange essentially unchanged.

Dehydration triggers anomalous subdiffusion in biomimetic cell membranes

This study shows that mild dehydration of biomimetic cell membranes causes reversible anomalous subdiffusion of lipids, indicating a shift in diffusion mode rather than membrane damage.

Mechanisms of viral budding through cellular membranes

The paper develops a theoretical model to explain how membrane geometry and boundary conditions influence viral budding completion, showing that vesicle-like geometries favor closure while flat membranes can stall.

Cardiolipin increases the peak of reversible traveling H+ fronts at the membrane surface

The paper investigates how cardiolipin (CL) affects H+ concentration and movement at mitochondrial membranes, finding that CL increases surface H+ activity and enables reversible acidification fronts.

Effects of Cholesterol on Nanodisc Formation and Magnetic Alignment in DMPC and Glycyrrhizic Acid Systems Probed by 31P and 14N Solid-State NMR

This study uses 31P and 14N solid-state NMR to analyze how cholesterol affects nanodisc formation and magnetic alignment in DMPC + glycyrrhizic acid systems, revealing optimal cholesterol concentrations for aligned bicelles.

Tunable electrostatic interactions of lipid-coated quantum dots with biological membranes

This study demonstrates lipid-coated quantum dots with tunable surface charge that enhance membrane interactions and labeling density in HEK293 cells, supported by molecular dynamics simulations.

ORB-TXTL: cell-free expression of membrane proteins on lipid bilayer-coated beads

This paper introduces ORB-TXTL, a cell-free platform for expressing membrane proteins on lipid bilayer-coated beads, demonstrating its utility for studying protein integration and reconstituting metabolic pathways.

Membrane Phase, Charge, and Curvature Regulate α-Synuclein Binding Dynamics

This study investigates how membrane phase, charge, and curvature jointly regulate α-synuclein binding dynamics using experimental and simulation methods.

Covalently linked peptides and membrane potential enable CyaA segment translocation

This study investigates the translocation mechanisms of two CyaA-derived peptides, showing that P454 translocates independently of membrane potential while P233 requires a negative potential, and their covalent linkage enables translocation without potential.

Ionizable Lipids Promote Curvature Remodeling and Altered Fluctuation Dynamics in Endosomal Membranes

This study investigates how ionizable lipids alter endosomal membrane properties to enhance endosomal escape, showing they reduce membrane rigidity and curvature.

Membrane Mimetic-Thermal Proteome Profiling Reveals Broad, Sequence-Independent Membrane Protein Stabilization by Cholesteryl Hemisuccinate

This paper uses MM-TPP to show that cholesteryl hemisuccinate broadly stabilizes membrane proteins across different systems and membrane mimetics.

Cooperative Regulation of Membrane Domain Registration by Lipid Headgroup Properties and Cholesterol Dynamics

This study uses molecular dynamics simulations to show how lipid headgroup properties and cholesterol concentration cooperatively regulate domain registration and anti-registration in model membranes.

Inward and Outward Tethers Read Out the Spontaneous Curvature of Cellular Membranes

This paper introduces a method to measure spontaneous curvature in cellular membranes using tether pulling and simulations, revealing baseline negative curvature and its modulation by treatments.

An On-Demand Nanodisc Platform for Reconstitution of Functional Membrane Proteins into Model and Living Membranes

This paper develops a nanodisc platform for rapidly reconstituting functional membrane proteins into model and living membranes, demonstrating its effectiveness with calcium channels and GPCRs.

A Bottom-Up Approach to Fungal Plasma Membrane Model: Lipid Mixture Design and Biophysical-Mechanical Characterization

The paper develops a fungal plasma membrane model using lipidomic data and biophysical characterization to mimic natural yeast vesicles for studying membrane properties and interactions.

Molecular Mechanisms Governing Peptide Nanodisc Assembly and Stability

This study uses molecular dynamics to uncover the assembly and stability mechanisms of peptide nanodiscs formed by 4F and identifies factors affecting their structure and function.

Cell surface remodeling caused by the loss of flippase subunit TMEM30A in immune cells

This study investigates the effects of TMEM30A loss on immune cell membrane dynamics, revealing PS externalization, proteome reorganization, and glycocalyx remodeling with implications for immune therapy.