Pluripotent Stem Cells Research

This study shows that Plakoglobin regulates naive pluripotency in mouse ESCs through mechanosensitivity, with expression specific to the blastocyst stage in human and mouse embryos.

This study shows that STAT3 phosphorylation variants regulate naïve pluripotency and developmental timing in mouse embryos, with loss of active STAT3 causing developmental delay and metabolic defects.

The paper demonstrates that TBLCs can efficiently generate blastocyst-like structures with 80% efficiency, outperforming EPSCs in blastoid formation.

The paper describes a method to generate reproducible single-rosette brain organoids from human pluripotent stem cells and uses them to study structural phenotypes in neurodevelopmental disorders.

The paper generates PRKN and PINK1-KO and double KO human iPSC lines using CRISPR/Cas9 to model Parkinson's disease.

The paper differentiates iPSCs from EA/TEF patients and healthy donors into esophageal organoids, identifying SOX2 and NKX2.1 dysregulation as potential causes of foregut compartmentalization defects.

This paper develops a cross-species platform to model human disease using genetically diverse mouse and human stem cells, showing that DYRK1A loss of function effects vary with genetic background.

This study identifies SAM68 as a regulator of cardiomyocyte differentiation by enhancing Gata4 translation through RNA-binding in mouse embryonic stem cells.

This study shows that SOX17 is not required for deriving and maintaining mouse XEN cell lines.

The paper successfully generates a rat-derived lung in a mouse model using a reverse-blastocyst complementation method, showing rat lung epithelial cells retain their developmental timing in the mouse body.

This study shows that Bcl-2 knockout in hiPSCs delays cardiomyocyte differentiation and reduces c-Myc expression, but not functionality in active areas.

This study shows Hbxip is essential for embryonic development and ESC differentiation by activating mTORC1 signaling.

This study identifies the cellular origins of mouse XEN cell lines, showing they derive from both extraembryonic endoderm and epiblast lineages in embryos.

This study shows that cyclic OKSM expression rejuvenates adult cardiomyocytes in mice with heart failure, improving systolic function without dedifferentiation.

This study identifies Platr4 as an ESC-specific lncRNA that regulates meso/endoderm lineage commitment by interacting with Tead4 to control Ctgf expression, leading to cardiac defects in knockout mice.

The paper shows that Irf1, controlled by the formative pluripotency network, regulates ISG expression and Oct6 in formative pluripotent cells.

The paper examines how MEK and GSK3 inhibition affects the methylome and transcriptome of mouse embryonic stem cells, revealing distinct epigenetic and transcriptional changes.

This study shows RAP1 modulates TIP60/p400 complex activity to regulate gene expression, increasing 2-cell-like cells in mouse embryonic stem cells.

This paper identifies 8-cell like cells (8CLCs) in human naive stem cells that model zygotic genome activation, expressing ZGA markers and transposable elements, and can be manipulated to enhance ZGA-like transcription.

The paper identifies 20 post-transcriptionally regulated genes essential for pluripotent stem cell survival, showing their protein upregulation and nuclear-to-cytoplasmic mRNA localization.

This paper develops a method to generate neuronal cells from adult fibroblasts and uses it to study gene expression and epigenetic changes in 22q11.2 deletion syndrome.

The paper develops a modular differentiation system to generate diverse human hindbrain and spinal neurons and uses computational methods to identify region-specific transcriptomic patterns.

This paper describes protocols to overcome ASH1L haploinsufficiency in stem cells, improving genome editing and neuronal differentiation efficiency.

This study shows OCT4 is essential for maintaining pluripotency in the epiblast and differentiation of the hypoblast in bovine embryos.

This study accelerates and standardizes the maturation of iPSC-derived neurons by optimizing extracellular cues, resulting in synchronized neurogenesis and earlier calretinin expression.

This study generates neural stem cells from fibroblasts using a single microRNA, achieving high purity and functionality in 2-3 days.

This study identifies and characterizes hPSC-derived FOXA2+ progenitor cells with enhanced ventricular cardiomyocyte differentiation potential.

This study uses human pluripotent stem cells to model lung development, generating renewable lung epithelial spheroids and identifying new cell lineage relationships.

This study identifies how TGF-β1 modulates cell fate in human ES cell-derived foregut endoderm by inhibiting multiple signaling pathways, promoting pancreas fate over liver.

This study shows that inhibiting N-myristoyltransferase (Nmt) promotes naive pluripotency in both mouse and human pluripotent stem cells.