Pluripotent Stem Cells Research

Gradient-Based Regulation of Activin A Directs Multilineage Liver Organoid Development from Human Pluripotent Stem Cells

This paper shows that adjusting Activin A levels in a 3D bioreactor system can generate liver-like structures from human stem cells, mimicking early liver development.

Reconstruction of the human nigrostriatal pathway in vitro reveals target-dependent dopamine neuron maturation

The paper introduces a human stem cell-based model of the nigrostriatal pathway that mimics in vivo connectivity and reveals target-dependent dopamine neuron maturation.

Simplified In Vitro Generation of Human Gastruloids for Modelling Early Development

This paper optimizes a protocol for generating human gastruloids by adjusting culture conditions, improving efficiency and cost-effectiveness for studying early development.

Multi-omic analysis reveals maturation programs in human pluripotent stem cell-derived cardiomyocytes during long-term culture

This study identifies molecular pathways and regulatory programs associated with hPSC-CM maturation through multi-omic profiling during long-term culture.

Generation of an induced pluripotent stem cell line from a patient with immune checkpoint inhibitor-induced myocarditis and concurrent type I diabetes

The paper describes the generation of an iPSC line from a patient with immune checkpoint inhibitor-induced myocarditis and type I diabetes, highlighting its pluripotency and potential for studying disease mechanisms.

Protracted fate acquisition and epigenetic de-aging during induced neural stem cell conversion of human blood cells

This study converts human blood cells into induced neural stem cells, showing prolonged epigenetic de-aging and delayed acquisition of neural stem cell identity over time.

Microgels enable iPSCs to assemble, expand, and differentiate into organoids -- from sizable to high throughput

This paper introduces a method using PEG microgels to enable reproducible, scalable iPSC organoid production and differentiation into specific tissues.

Growth Factor-Based Manufacturing of Human Pluripotent Stem Cell-Derived Cardiomyocytes Using the Vertical Wheel Bioreactor System

This paper develops a scalable bioreactor-based method using growth factors to produce human pluripotent stem cell-derived cardiomyocytes with high purity and yield.

Navigating Human Astrocyte Differentiation: Direct and Rapid one-step Differentiation of Induced Pluripotent Stem Cells to Functional Astrocytes Supporting Neuronal Network development

This study presents a one-step protocol to differentiate hiPSCs into functional astrocytes, enabling co-culture with neurons to support network development.

Self-amplifying RNA enables rapid, durable, integration-free programming of hiPSCs

This paper demonstrates that self-amplifying RNA (saRNA) enables durable, integration-free transgene expression in hiPSCs, supporting cell-fate programming and functional reporter analysis.

New iPSC resource with long-read whole genome sequencing characterizations for enhanced in vitro modeling

The paper introduces a new iPSC resource with long-read whole-genome sequencing data for in vitro modeling and genomic analysis.

Generation of effector CD4+ T cells from Human iPSC

This paper generates functional CD4+ T cells from human iPSC by modulating Notch signaling, showing cytokine diversity and transcriptional similarity to blood CD4+ T cells.

Transcriptional landscape of direct reprogramming toward the hematopoietic lineage

The paper develops a single-cell transcriptomic reference atlas and evaluates a transcription factor recipe for reprogramming fibroblasts into hematopoietic stem cells, revealing intermediate transcriptomic states and transcriptional remodeling.

Derivation of human post-mitotic cardiomyocytes from tetraploid iPSCs

This study generates tetraploid iPSCs to produce more mature human cardiomyocyte-like cells with improved characteristics compared to conventional iPSC-derived cardiomyocytes.

TGF-β Signalling Drives Chemotaxis of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Response to MI Stimulus

This study shows hiPSC-CMs migrate toward MI tissue, driven by TGF-β signalling, and identifies TGF-β as a key mediator of this chemotactic response.

TGFβ-dependent upregulation of OCIAD2 is essential for epithelial-to-mesenchymal transition during mesendoderm differentiation

This study shows OCIAD2 upregulation during mesendoderm differentiation is TGFβ-dependent and essential for EMT, with implications for lineage commitment and tumor progression.

ERH elicits cell lineage restriction in mammalian preimplantation development and differentiation from pluripotency via H3K9me3-heterochromatin

This study shows ERH maintains H3K9me3 heterochromatin in mammalian cells, restricting lineage potential and repressing pluripotency genes during development and reprogramming.

Xeno-free human iPSC-derived prostate organoid platform for multilineage differentiation and genetic manipulation

This paper develops a xeno-free human iPSC-derived prostate organoid platform that enables multilineage differentiation and genetic manipulation for studying prostate biology and disease.

Modeling physiological insulin sensitivity and pre-diabetic insulin resistance in multiple hPSC-derived cell types

The paper develops a protocol to model insulin sensitivity and resistance in multiple hPSC-derived cell types, showing insulin resistance across various cell types under diabetic conditions.

A Universal 6iL/E4 Culture System for Deriving and Maintaining Embryonic Stem Cells Across Mammalian Species

The paper develops a serum-free 6iL/E4 culture system that enables derivation and maintenance of embryonic stem cells (ESCs) across multiple mammalian species, revealing conserved mechanisms for ESC self-renewal.

Isthmin-1 is a Key Regulator of Induced Pluripotent Stem Cell-Derived Cardiomyocytes Maturation through Activation of p53 Signaling

This paper identifies ISM1 as a key regulator of iPSC-CM maturation through p53 activation, improving structural and metabolic features.

Generation of spermatogonia from pluripotent stem cells in humans and non-human primates

This study generates spermatogonia from human and non-human primate pluripotent stem cells using xenogeneic testes, providing models for male infertility research.

Generation of three induced pluripotent stem cell lines from an immune checkpoint inhibitor-induced myocarditis patient and controls

The paper generates three iPSC lines from an ICIM patient and controls to study disease mechanisms and develop therapies.

A PERTURBATION CELL ATLAS OF HUMAN INDUCED PLURIPOTENT STEM CELLS

The paper generates a CRISPRi perturbation cell atlas in human induced pluripotent stem cells, identifies novel regulators of pluripotency and metabolism, and proposes a machine learning framework for predicting perturbation recipes.

WNT signaling in human pluripotent stem cells promotes HDAC2-dependent epigenetic programs and development of retinoic acid-responsive mesoderm

This study shows that WNT signaling in hPSCs promotes mesoderm development through HDAC2-dependent epigenetic programs and RA responsiveness, enhancing T cell generation.

Stage-adaptive integration of polydopamine promotes hPSC-derived alveolar organoids differentiation and maturation

This study shows that stage-adaptive integration of polydopamine coatings and nanoparticles enhances the maturation and secretory function of hPSC-derived alveolar organoids.

Engineering functional ventral midbrain dopaminergic neurons in human organoids through WNT modulation and bioreactor culture

This paper develops a method to generate human midbrain organoids enriched in SNpc-like dopaminergic neurons using WNT modulation and bioreactor culture, showing improved functionality and vulnerability to Parkinson's disease pathology.

iSCORE-PD: an isogenic stem cell collection to research Parkinson Disease

The paper generates a collection of 65 isogenic human stem cell lines with PD-associated mutations to study Parkinson's disease, revealing that most genetic variation comes from cell culture rather than genome editing.

Efficient multi-lineage cardiovascular differentiation of human pluripotent stem cells in animal serum-free conditions

This study develops a serum-free method for efficient differentiation of human pluripotent stem cells into cardiac lineages and evaluates engineered heart tissue fabrication.

Scalable Hypothalamic Arcuate Neuron Differentiation from Human Pluripotent Stem Cells Suitable for Modeling Metabolic and Reproductive Disorders

This paper describes a scalable method to differentiate human pluripotent stem cells into hypothalamic arcuate neurons, which can model metabolic and reproductive disorders.