Toxoplasma gondii Research Studies

A Toxoplasma gondii putative arginine transporter localizes to the plant-like vacuolar compartment and controls parasite extracellular survival and stage differentiation

This study identifies TgAAT1, a putative arginine transporter in Toxoplasma gondii, which localizes to the PLVAC and is essential for extracellular survival and bradyzoite differentiation.

Mapping host-microbe transcriptional interactions by dual perturb-seq

This paper introduces dual perturb-seq, a method combining CRISPR screening and single-cell RNA-sequencing to identify host-microbe transcriptional interactions, revealing new effector proteins in Toxoplasma gondii.

Toxoplasma gondii aspartic protease 5 (TgASP5): Understanding structural details and inhibition mechanism.

This study investigates the structure and inhibition mechanism of TgASP5, identifying potential inhibitors for developing therapeutic strategies against toxoplasmosis.

Complement-dependent loss of inhibitory synapses on pyramidal neurons following Toxoplasma gondii infection

This study shows that Toxoplasma gondii infection causes complement-dependent loss of inhibitory synapses on excitatory pyramidal neurons in the neocortex.

IFN-γ stimulated murine and human neurons mount anti-parasitic defenses against the intracellular parasite Toxoplasma gondii

This study shows that IFN-γ stimulated murine and human neurons can clear Toxoplasma gondii parasites, challenging the dogma that neurons cannot mount anti-parasitic defenses.

Mechanisms regulating reactivation pathways of Toxoplasma gondii as revealed by bradyzoite transgenesis.

The paper develops ex vivo bradyzoite-based methods to generate transgenic Toxoplasma gondii and identifies gene functions in bradyzoite reactivation pathways.

Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in Toxoplasma gondii by CRISPR-Cas9

The paper develops single and duplex TaqMan qPCR methods to accurately quantify integrated selection markers in Toxoplasma gondii CRISPR-Cas9 knockouts.

Cell-based screen identifies human type I interferon-stimulated regulators of Toxoplasma gondii Infection

The paper uses a CRISPR/Cas9 screen to identify type I interferon-stimulated genes that regulate Toxoplasma gondii infection in macrophages.

A unique Toxoplasma gondii haplotype under strong selection has accompanied domestic cats in their global expansion

The paper identifies a unique Toxoplasma gondii haplotype associated with domestic cats and shows it has been selected for its role in sexual reproduction in domestic cats during their global expansion.

A novel fragmented mitochondrial genome in the protist pathogen Toxoplasma gondii and related tissue coccidia

The paper reports a novel fragmented mitochondrial genome in Toxoplasma gondii and related tissue coccidia, revealing a unique genome architecture and cytochrome gene structure.

Reevaluation of the Toxoplasma gondii and Neospora caninum genomes reveals misassembly, karyotype differences and chromosomal rearrangements

The paper reevaluates the genomes of Toxoplasma gondii and Neospora caninum, revealing misassembly, chromosomal rearrangements, and new genes, with implications for genome-based comparative biology.

Third generation sequencing revises the molecular karyotype for Toxoplasma gondii and identifies emerging copy number variants in sexual recombinants.

This paper uses third-generation sequencing to revise the molecular karyotype of Toxoplasma gondii, identifying chromosome fusions and copy number variants in sexual recombinants.

Virulence shift in Type X Toxoplasma gondii: natural cross QTL identifies ROP33 as rodent Vir locus

The paper identifies ROP33 as a murine virulence gene in Type X Toxoplasma gondii strains isolated from southern sea otters, showing differential pathogenicity across hosts.

Morphological and biochemical repercussions of Toxoplasma gondii infection in a 3D human brain neurospheres model

This study investigates the effects of Toxoplasma gondii infection on a 3D human brain model, showing changes in morphology, gene expression, and inflammation.

Single-cell RNA-seq reveals CD16- monocytes as key regulators of human monocyte transcriptional response to Toxoplasma

The paper uses single-cell RNA sequencing to show that CD16- monocytes are key in the transcriptional response of human monocytes to Toxoplasma infection, revealing transcriptional heterogeneity and cytokine secretion patterns.

Influence of Activating and Inhibitory Killer Immunoglobulin-Like Receptors (KIR) genes on the recurrence rate of ocular toxoplasmosis in Brazil

The paper investigates the association between KIR gene polymorphism and recurrence rates of ocular toxoplasmosis in Brazilian patients, finding protective and susceptibility markers.

Transcriptional profiling reveals T cells cluster around neurons injected with Toxoplasma gondii proteins

The paper uses RNA-seq to compare transcriptional profiles of neurons infected with Toxoplasma gondii, bystander neurons, and controls, finding immune cell transcripts enriched in infected neurons.

Toxoplasma induces stripping of perisomatic inhibitory synapses

This study shows that Toxoplasma gondii infection causes loss of perisomatic inhibitory synapses through microglia-mediated synaptic stripping.

Apicomplexan motility depends on the operation of an endocytic-secretory cycle

This study shows that endocytosis is essential for gliding motility in Toxoplasma gondii, as it enables retrograde membrane flow and the recycling of surface proteins.

Toxoplasma gondii ROP18 Inhibits Human Glioblastoma Cell Apoptosis through Mitochondrial Pathway by Targeting Host Cell P2X1

Impaired social behaviour and molecular mediators of associated neural circuits during chronic Toxoplasma gondii infection in female mice

This study examines how chronic Toxoplasma gondii infection affects social behavior and neural circuits in female mice, identifying molecular changes in brain regions associated with social behavior.

Latent Toxoplasma gondii infection increases soluble mutant huntingtin and promotes neurodegeneration in the YAC128 mouse model of Huntington's disease

This study shows that latent Toxoplasma gondii infection worsens neurodegeneration in YAC128 HD mice by increasing soluble mutant huntingtin and neurofilament light protein levels.

Engineering Brain Parasites for Intracellular Delivery of Therapeutic Proteins

This paper engineers Toxoplasma gondii to deliver therapeutic proteins to the CNS by fusing them to the parasite's secretory proteins, demonstrating potential for intracellular delivery of treatments for neurological disorders.

Radial Glia cell infection by Toxoplasma gondii disrupts brain microvascular endothelial cell integrity

This study shows that Toxoplasma gondii infection of radial glia cells reduces their proliferation and neurogenesis, disrupts endothelial cell barrier function through cytokine changes, and impairs neurovascular interactions.

Toxoplasmosis, but not borreliosis, is associated with psychiatric disorders: A cross-sectional survey on 46 thousand of subjects

The paper investigates associations between toxoplasmosis and borreliosis infections and psychiatric disorders in 46,000 subjects, finding stronger links with toxoplasmosis for several mental health conditions.

Physical And Mental Health Status In Toxoplasma-Infected Women Before And Three Years After They Learn About Their Infection: The Role Of Rh Factor

The paper compares physical and mental health status of Toxoplasma-infected and uninfected women before and three years after infection disclosure, finding poorer health in infected women regardless of Rh factor.

Intragenomic Redistribution Of Host Transcription Factor Binding With Toxoplasma gondii Infection

This study investigates how Toxoplasma gondii infection alters host transcriptional regulation by redistributing AP-1 transcription factor binding, leading to changes in chromatin accessibility and gene expression.