Toxoplasma gondii Research Studies

A MultiOmics time-resolved systems map of gene regulation in Toxoplasma gondii

This study creates a time-resolved multi-omics map of gene regulation in Toxoplasma gondii by integrating perturbation data with single-cell measurements, revealing regulatory networks and novel interactions.

Tissue-specific transcriptomic responses to early Toxoplasma gondii infection in sheep during congenital toxoplasmosis

This study examines early transcriptomic responses to Toxoplasma gondii infection in pregnant sheep, identifying tissue-specific immune and stress pathways linked to fetal loss.

A transporter essential for apical secretory organelles homeostasis in Toxoplasma gondii

This study identifies TFP5 as a transporter critical for lipid homeostasis in apical secretory organelles of Toxoplasma gondii, impacting parasite virulence.

CD4+ T cell-intrinsic T-bet sustains protective CNS immunity and parasite control during Toxoplasma gondii infection

This study shows that CD4+ T cell-intrinsic T-bet is essential for sustaining CNS immunity and parasite control during chronic Toxoplasma gondii infection, but not for acute stages.

APEX2 proximity labeling maps the mGBP2-proximal proteome at the Toxoplasma gondii parasitophorous vacuole

The paper uses APEX2 proximity labeling to identify mGBP2-associated proteins at the Toxoplasma gondii parasitophorous vacuole and finds COX2 is critical for parasite survival.

Histone H2A.X contributes to Gene Expression Regulation and Cell-Cycle Progression in Toxoplasma gondii Tachyzoites

This study shows that H2A.X in Toxoplasma gondii is not essential for DNA damage response but regulates cell cycle progression and virulence gene expression.

Pentachloropseudilin disrupts multiple steps of the Toxoplasma gondii lytic cycle, consistent with predicted multi-myosin engagement

This paper shows pentachloropseudilin disrupts multiple stages of the Toxoplasma gondii lytic cycle by interfering with different myosins, suggesting it as a tool for studying myosin functions and a potential antiparasitic strategy.

Toxoplasma gondii GRA66 prevents premature egress driven by the host phospholipase RARRES3, independently of RNF213

Organization of Myosin H in the Apical Complex of Toxoplasma Gondii Revealed by 3D Single-Molecule Super-Resolution Microscopy

This paper uses 3D single-molecule super-resolution microscopy to determine the orientation of Myosin H in the apical complex of Toxoplasma gondii, revealing differences in radial organization between its N- and C-termini.

A Suite of Eight Toxoplasma gondii Effectors Cooperates to Activate the Non-canonical NF-κB Pathway

This paper shows that Toxoplasma gondii uses eight effectors to activate the non-canonical NF-κB pathway in host cells through a collaborative network.

Dual apical methyltransferases orchestrate motility initiation in apicomplexan parasites

The paper identifies two methyltransferases, PCKMT and AKMT, that regulate motility initiation in apicomplexan parasites through coordinated actin nucleation and force propagation.

The live attenuated DGAT1-knockout whole-cell Toxoplasma vaccine confers protective immunity against acute and chronic toxoplasmosis

This paper develops a live-attenuated Toxoplasma vaccine by knocking out DGAT1, showing it provides long-term protection against both acute and chronic toxoplasmosis.

SecretTarget: A pipeline for identifying host-interacting effector candidates through secondary localization features

SecretTarget identifies host-interacting effector candidates using secondary localization features, revealing a novel host ER-parasite interaction critical for parasite survival.

Parasitophorous vacuole membranes of Toxoplasma gondii and Plasmodium falciparum lack the lipid asymmetry characteristic of host cell plasma membranes

The paper analyzes lipid asymmetry in parasitophorous vacuole membranes of Toxoplasma gondii and Plasmodium falciparum, finding symmetric distribution of key lipids unlike host cell membranes.

Short linear motifs - Underexplored players driving Toxoplasma gondii infection

The paper identifies short linear motifs in Toxoplasma gondii secreted proteins using a computational pipeline and validates some predictions experimentally.

A proximity biotinylation approach for the identification of membrane contact site proteins in Toxoplasma gondii

The paper uses proximity biotinylation to identify membrane contact site proteins in Toxoplasma gondii, validating a candidate involved in apicoplast-mitochondrion interactions.

Toxoplasma gondii Fatty Acid Elongases are Important for Virulence and Persistence

This paper investigates the role of fatty acid elongases (FAE) in Toxoplasma gondii type II strains, showing their importance for virulence, persistence, and lipid metabolism under stress.

Toxoplasma GRA8 engages the host ESCRT accessory protein ALG-2 and is necessary for parasite metabolic integrity

This paper identifies TgGRA8 as a Toxoplasma protein that recruits ALG-2 to the parasitophorous vacuole, essential for parasite metabolism and ESCRT hijacking.

Toxoplasma TgATG2 works with TgATG9 and TgProp1 to drive autophagic flux for parasite extracellular survival and persistence.

This paper identifies TgATG2 as a key component of the Toxoplasma gondii autophagy machinery required for bradyzoite survival and cyst persistence.

A pooled image-based CRISPR screen identifies EAF1 as a T. gondii modulator of ESCRT subversion.

The paper identifies EAF1 as a Toxoplasma gondii effector that modulates ESCRT subversion using a pooled image-based CRISPR screening framework called spaCR.

ABCE1-dependent translational control links Fe-S cluster biogenesis to parasite growth and lipid homeostasis in Toxoplasma gondii

This study shows that ABCE1 is essential for parasite growth and lipid homeostasis in Toxoplasma gondii by linking translational control to Fe-S cluster biogenesis.

Cysteine-S-nitrosylation inhibits Rop5-mediated immune evasion in Toxoplasma gondii

This study shows that RNS inhibit ROP5-mediated immune evasion in Toxoplasma gondii by modifying ROP5 through S-nitrosylation, reducing its association with the parasitophorous vacuole membrane.

The human parasite, Toxoplasma gondii, is paralyzed without two components of the apical polar ring

This study identifies APR9 and KinesinA as essential components of the apical polar ring in Toxoplasma gondii, showing that their combined loss paralyzes the parasite and impairs key life cycle processes.

Comparison of Four Density Gradient Centrifugation Protocols for Purification of Toxoplasma gondii PRU Brain Cysts

The paper compares four density gradient centrifugation methods for purifying Toxoplasma gondii brain cysts, finding that Percoll, CsCl, and sucrose yield more cysts than LSM, with varying viability and infectivity outcomes.

Identification of UCB-9721 as a potent inhibitor of MyoA, the essential class XIV myosin motor of apicomplexan parasites

This paper identifies UCB-9721 as a potent inhibitor of T. gondii MyoA, showing greater potency than KNX-002 and inhibiting related apicomplexan parasites.

Characterization of Ovine Abortion in Uruguay Reveals Extensive Non-Clonal Diversity and Multiple Evolutionary Origins of Toxoplasma gondii

This study characterizes Toxoplasma gondii strains causing ovine abortion in Uruguay, revealing non-clonal diversity and multiple evolutionary origins linked to human infections.

Legacy 4(1H)-quinolone scaffolds activity against acute and chronic Toxoplasma gondii infection

This study evaluates two 4(1H)-quinolone compounds for their efficacy against both acute and chronic Toxoplasma gondii infection, showing potent activity against tachyzoites and bradyzoites.

TGIF is a golgin-like protein required for Golgi structural maintenance and function in Toxoplasma gondii

This study identifies TGIF as a Golgi-associated protein essential for Golgi structure and function in Toxoplasma gondii, showing its role in secretory pathway organization.

Toxoplasma IMC1 is a central component of the subpellicular network and plays critical roles in parasite morphology, replication, and infectivity

This study identifies IMC1 as a critical component of the Toxoplasma gondii inner membrane complex, showing its role in morphology, replication, and infectivity through expression timing, knockout, and interaction analyses.

Evolutionary Remodeling of Ubiquinone Biosynthesis in Toxoplasma gondii Reveals an Essential Bi-functional Monooxygenase

The paper identifies a unique bi-functional monooxygenase in Toxoplasma gondii essential for ubiquinone biosynthesis and presents inhibitors targeting this enzyme.