Zebrafish Biomedical Research Applications

Single cell RNA-seq analysis of spinal locomotor circuitry in larval zebrafish

This study uses single-cell RNA sequencing to identify molecular differences between motoneuron types in larval zebrafish that contribute to their roles in swimming behavior.

Plasticity of the dopaminergic phenotype and of locomotion in larval zebrafish induced by changes in brain excitability during the embryonic period

The paper shows that modulating Embryonic Neuronal Excitability (ENE) in zebrafish embryos affects dopaminergic neuron numbers and larval locomotion, with implications for neurodevelopmental disorders.

Effect of caldesmon mutations in the development of zebrafish embryos.

The paper examines the effects of caldesmon mutations in zebrafish embryos, finding mild developmental impacts and normal gross development, suggesting caldesmon may be a viable cancer therapy target.

Progenitor derived glia are required for spinal cord regeneration in zebrafish

This study identifies the role of progenitor-derived glia in zebrafish spinal cord regeneration through genetic and molecular analyses.

Roles of the 5-HT2C receptor on zebrafish sociality

This study investigates the role of 5-HT2C receptors in zebrafish social behavior using pharmacological manipulations and behavioral tests.

Transgenerational Effects of Early-Life Stress on Anxiety in Zebrafish (Danio rerio)

This study shows that early-life stress in zebrafish affects anxiety-related behaviors and these effects are transgenerational across three generations.

Hemato-vascular specification requires arnt1 and arnt2 genes in zebrafish embryos

This study shows that arnt1 and arnt2 genes are required alongside npas4l for hemato-vascular specification in zebrafish embryos, with each gene necessary but not sufficient alone.

Acute administration of a dopamine D2/D3 receptor agonist alters behavioral and neural parameters in adult zebrafish

This study examines the effects of quinpirole, a dopamine D2/D3 receptor agonist, on behavior and neurotransmitter levels in adult zebrafish, finding altered locomotion, anxiety, memory, and neurotransmitter profiles.

Efficient knock-in method enabling lineage tracing in zebrafish

The paper introduces an efficient CRISPR-Cas9-based knock-in method in zebrafish for lineage tracing and generates reporter lines for specific cell types.

From Multi-Allele Fish to Non-Standard Environments, How ZFIN Assigns Phenotypes, Human Disease Models, and Gene Expression Annotations to Genes

The paper describes how ZFIN annotates phenotype, expression, and disease model data in zebrafish research across different experimental designs.

Neural circuits underlying habituation of visually evoked escape behaviors in larval zebrafish

The paper identifies three distinct neural pathways in larval zebrafish that mediate habituation to dark looming stimuli, with one pathway specifically inhibiting escape responses.

Examining craniofacial variation among crispant and mutant zebrafish models of human skeletal diseases

This study evaluates a semi-automated tool to quantify craniofacial variations in zebrafish models of human skeletal diseases, finding phenotypic differences for four genes and parallels to human symptoms for two.

Actomyosin contractility in olfactory placode neurons opens the skin epithelium to form the nostril

The paper shows that actomyosin contractility in olfactory placode neurons causes the skin epithelium to rupture, forming the nostril in zebrafish.

Generation of a transparent killifish line through multiplex CRISPR/Cas9-mediated gene inactivation

The paper generates a transparent killifish line by inactivating three pigmentation genes, enabling in vivo studies of aging and behavior.

Pleiotropic contribution of rbfox1 to psychiatric and neurodevelopmental phenotypes in a zebrafish model

This study investigates the behavioral effects of rbfox1 deficiency in zebrafish, finding multiple behavioral changes that resemble those in Rbfox1-deficient mice and psychiatric patients.

A single-cell resolution gene expression atlas of the larval zebrafish brain

The paper creates a single-cell gene expression atlas of the larval zebrafish brain using HCR, identifies a novel cerebellar cell type, and integrates gene expression with functional activity maps.

The roles of NADPH oxidases during adult zebrafish fin regeneration

This study identifies Duox as a key NADPH oxidase for ROS production and fin regeneration in adult zebrafish, with secondary roles for Nox1-4 and a circadian rhythm in ROS levels.

Characterization of the zebrafish gabra1sa43718/sa43718 germline loss of function allele confirms a function for Gabra1 in motility and nervous system development.

The paper characterizes a zebrafish gabra1 loss of function allele, showing decreased mRNA expression and seizure-like phenotypes, supporting previous findings on GABRA1's role in nervous system development.

Scalable Apparatus to Measure Posture and Locomotion (SAMPL): a high-throughput solution to study unconstrained vertical behavior in small animals

The paper introduces SAMPL, a high-throughput system for measuring posture and locomotion in small animals, demonstrating its use in zebrafish and showing genetic and developmental effects on movement.

Next-generation plasmids for transgenesis in zebrafish and beyond

The paper introduces next-generation transgenesis tools for zebrafish and other species, including vectors with fluorescent markers and promoters for specific tissue expression.

Ten years of unpredictable chronic stress in zebrafish: a systematic review and meta-analysis

This review meta-analyzes 38 studies on unpredictable chronic stress in zebrafish, finding increased anxiety/fear and cortisol, decreased locomotor function, but no significant effect on social behavior, while highlighting methodological issues.

Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish

This study identifies Zbtb14 as a regulator of monocyte and macrophage development by inhibiting PU.1 expression in zebrafish, with implications for myeloid disorders.

The Digital 3D-Atlas MAKER (DAMAKER): a dynamic and expandable digital 3D-tool for monitoring the temporal changes in tissue growth during hindbrain morphogenesis

The paper introduces DAMAKER, a 3D tool for tracking tissue growth and neuronal differentiation in zebrafish hindbrain over time using in vivo imaging and segmentation.

A cerebellar-prepontine circuit for tonic immobility triggered by inescapable threat

This study identifies a cerebellar-prepontine circuit that mediates tonic immobility in larval zebrafish in response to inescapable threats.

A dual involvement of Protocadherin-18a in stromal cell development guides the formation of a functional hematopoietic niche.

This study shows that Pcdh18a in stromal cell progenitors is essential for hematopoietic niche formation in zebrafish.

Alone in a crowd: Effect of a nonfunctional lateral line on expression of the social hormone parathyroid hormone 2.

This study shows that zebrafish with nonfunctional lateral lines have reduced pth2 expression in social contexts, suggesting they experience isolation despite being raised socially.

Whole-brain optical access in small adult vertebrates with two- and three-photon microscopy

This paper demonstrates that two- and three-photon microscopy can access the entire adult Danionella brain without modifications, enabling whole-brain imaging in live adult vertebrates.

Thyroid Ablation Alters Passive Stiffness and Swimming Kinematics in Zebrafish

This study shows that thyroid hormone affects zebrafish swimming mechanics by altering passive stiffness and kinematic patterns during development.

Generation of floxed alleles for cell-specific knockout in zebrafish

The paper develops a protocol to generate floxed alleles in zebrafish for cell-specific knockout, demonstrating its utility in studying gene function.

Lateral line hair cells integrate mechanical and chemical cues to orient navigation

This study shows zebrafish lateral line hair cells detect serotonin, influencing navigation behavior in response to environmental cues.