Zebrafish Biomedical Research Applications

Duplicated zebrafish (Danio rerio) inositol phosphatases inpp5ka and inpp5kb diverged in expression pattern and function

The paper shows that zebrafish inpp5ka and inpp5kb, duplicated from whole genome duplication, have diverged in expression and function, with inpp5ka more closely related to human INPP5K and inpp5kb losing catalytic activity.

Stac1 Regulates Sensory Stimulus Induced Escape Locomotion

This study identifies stac1 in zebrafish embryos and shows it is required for sensory stimulus-induced escape locomotion via regulation of CSF-cNs.

Reduced wound healing and angiogenesis in aged zebrafish and turquoise killifish.

This study shows that aged zebrafish and turquoise killifish exhibit reduced fin regeneration and angiogenesis, with VEGF-A expression and vascular density changes, suggesting a key role for angiogenesis in aging wound healing.

V2b neurons act via multiple targets in spinal motor networks

This study maps the connectivity of V2b neurons in larval zebrafish spinal circuits, showing they inhibit motor neurons and other interneurons, with patterned inhibition along the rostrocaudal axis.

zFACE: Facial Analytics from a Coordinate Extrapolation System for Developing Zebrafish

The paper introduces zFACE, a method for analyzing craniofacial development in zebrafish embryos, showing that smarca4a loss causes craniofacial anomalies similar to human Coffin-Siris syndrome.

Early pre-neural serotonin modulates balance of late monoamines and behavioral patterns in fish model system

The paper shows that early pre-neural serotonin in zebrafish affects later serotonin synthesis and behavioral patterns without causing major developmental abnormalities.

A primer genetic toolkit for exploring mitochondrial biology and disease using zebrafish

The paper presents a collection of zebrafish genetic mutants for studying mitochondrial biology and disease.

Visual recognition of social signals by a tecto-thalamic neural circuit

The paper identifies a tecto-thalamic neural circuit that processes visual social signals and drives social attraction in zebrafish.

What do zebrafish prefer? Directional and color preferences in maze tasks

This study investigates innate directional and color preferences in male and female zebrafish using maze tasks, finding sex differences in color preference but not direction.

A viral toolbox for conditional and transneuronal gene expression in zebrafish

The paper develops viral tools for conditional and transneuronal gene expression in zebrafish to study neuronal circuits.

Macrophages break interneuromast cell quiescence by intervening the inhibition of Schwann cells in zebrafish lateral line

The paper shows macrophages help regenerate neuromasts in zebrafish by disrupting Schwann cell inhibition of interneuromast cells during injury.

Zebrafish fin regeneration requires generic and regeneration-specific responses of osteoblasts to trauma

This study shows that osteoblast dedifferentiation and migration are distinct, differentially regulated processes required for zebrafish fin regeneration.

Zebrafish Cre/lox regulated UFlip alleles generated by CRISPR/Cas targeted integration provide cell-type specific conditional gene inactivation

The paper develops a CRISPR/Cas9-based method to generate Cre/lox regulated conditional gene alleles in zebrafish, enabling cell-type specific gene inactivation.

VALPROIC ACID AFFECTS NEURONAL SPECIFICATION AND DIFFERENTIATION DURING EARLY OPTIC TECTUM DEVELOPMENT OF ZEBRAFISH

This study examines the effects of valproic acid on zebrafish optic tectum development, showing impaired neuronal specification and neurite extension.

Temporally and regionally distinct morphogenetic processes govern zebrafish tail fin blood vessel network expansion

The paper identifies temporally and spatially distinct morphogenetic processes in zebrafish tail fin vascular development.

Longitudinal brain studies in adult zebrafish by MRI

This paper introduces a method to image adult zebrafish brains using MRI for longitudinal studies, including a 3D-printed life support chamber and optimized contrast agent delivery.

New functional vessels form after spinal cord injury in zebrafish

This study shows zebrafish can re-vascularize spinal tissue after injury, with early angiogenesis and barrier restoration, but Vegfr2 inhibition does not block vessel formation.

A structural atlas of the developing zebrafish telencephalon based on spatially-restricted transgene expression

The paper maps the development of cell populations in the zebrafish telencephalon during eversion using transgene expression and creates a structural atlas.

Growth orientations, rather than heterogeneous growth rates, dominate jaw joint morphogenesis in the larval zebrafish

This study uses computational modeling to show that growth orientation, not growth rate, primarily drives jaw joint morphogenesis in larval zebrafish.

Deficiency of the ywhaz gene, involved in neurodevelopmental disorders, alters brain activity and behaviour in zebrafish

This study shows that ywhaz gene deficiency in zebrafish affects brain activity and behavior, with altered neuronal connectivity and monoamine levels.

A combined human in silico and CRISPR/Cas9-mediated in vivo zebrafish based approach for supporting gene target validation in early drug discovery

The paper identifies three genes associated with heart failure using human exome sequencing and validates their roles in cardiovascular function using zebrafish CRISPR/Cas9 mutagenesis.

Using light-sheet microscopy to study spontaneous activity in the developing lateral-line system

This study uses light-sheet microscopy to investigate spontaneous activity in the developing zebrafish lateral-line system, showing that hair cells and surrounding cells autonomously generate spontaneous calcium activity.

Follow that cell: leukocyte migration in L-plastin mutant zebrafish.

This study examines the role of L-plastin in macrophage migration and morphology in zebrafish, finding that its absence impairs migration speed and directionality.

Coagulation Factor IIIa (f3a) Knockdown in Zebrafish Leads to Defective Angiogenesis and Mild Bleeding Phenotype

This paper investigates the role of f3a in zebrafish development, finding that its knockdown causes defective angiogenesis and mild bleeding.

Regenerating vascular mural cells in zebrafish fin blood vessels are not derived from pre-existing ones and differentially require pdgfrb signaling for their development

Evolutionary divergence of locomotion in two related vertebrate species

This study compares swimming patterns and neuronal circuits in two closely related fish species to understand how environmental factors drive evolutionary divergence in locomotion.

V3 Interneurons are Active and Recruit Spinal Motor Neurons During In Vivo Fictive Swimming in Larval Zebrafish

The paper shows V3 interneurons are active during fictive swimming in larval zebrafish and contribute to locomotor amplitude but not frequency.

Integration of vascular progenitors into functional blood vessels represents a novel mechanism of vascular growth

The paper identifies a novel site of late vasculogenesis in zebrafish embryos that contributes to functional blood vessels after circulation is established.

Brain-wide visual habituation networks in wild type and fmr1 zebrafish

The paper uses calcium imaging to study brain-wide visual habituation networks in zebrafish, finding altered network dynamics in fmr1 mutants linked to slower habituation.

Zebrafish Information Network, the knowledgebase for Danio rerio research

The paper describes ZFIN, a central repository for zebrafish genetic and genomic data, emphasizing its role in curating, integrating, and making data FAIR for research.