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Exploring diverse routes to high-affinity-antibody variable domains through deep-sequencing-informed machine learning

This study uses deep sequencing and machine learning to optimize antibody discovery by analyzing training data and search space design for high-affinity VHHs.

Short single-stranded DNA oligonucleotides enable intracellular transcription of functional RNAs in mammalian cells

This paper introduces a method using single-stranded DNA templates and bacteriophage RNA polymerases to produce functional RNAs in mammalian cells, demonstrating applications in CRISPR-based genome editing.

A Growth-Based Framework for Scaling Infant Anthropometry in Motion Capture Studies

This paper develops a growth-adjusted anthropometric scaling framework to improve biomechanical analysis of infants by integrating CDC growth charts with motion capture data.

High-density Lipoprotein Inspired Lipid Nanoparticles for Systemic RNA delivery to the Brain

This study develops HDL-inspired lipid nanoparticles for targeted RNA delivery to the brain, showing improved BBB transport and cell-type-specific therapeutic effects in mouse models.

AI-enhanced cardiac digital twins extend drug proarrhythmic risk assessment through experimental data uncertainty propagation and overdose exploration: a loperamide case study

The paper uses AI-enhanced cardiac digital twins to assess drug proarrhythmic risk by propagating experimental uncertainty and simulating extreme exposures, finding sex-specific arrhythmogenic thresholds for loperamide.

Synthesis and mechanical characterization of polyacrylamide (PAAm) hydrogels with different stiffnesses for large-batch cell culture applications

This paper provides a step-by-step protocol for synthesizing and characterizing PAAm hydrogels of varying stiffness for cell culture and mechanobiology studies.

Precision Fermentation of Recombinant Myofibrillar Proteins for Future Foods

This study produces recombinant myofibrillar proteins (actin and myosin) via precision fermentation, achieving higher yields and purity than prior methods, and shows improved textural properties when combined with plant-based proteins.

In Situ Graphene-Seq: Spatial Transcriptomics and Chronic Electrophysiological Characterization of Tissue Microenvironments

This paper introduces in situ graphene-seq, a platform integrating chronic electrophysiology with spatial transcriptomics to analyze tissue microenvironments, showing how spatial heterogeneity affects cell function and gene expression.

An IgG-Optimized Enzyme-Linked Lectin Assay (ELLA) for Quantitative Analysis of Immunoglobulin Glycosylation

The paper develops an optimized ELLA assay for quantifying IgG glycosylation, improving reproducibility and cross-study comparability through standardized protocols and a quantitative surrogate standard.

Quantifying the Localization of Histological Staining Markers within the GI Epithelial Unit Axis: A Gastrointestinal Spatial Pathology Plugin for ImageJ

Mitochondria Clearance Enables Macrophage-Driven Maturation of iPSC-Derived Cardiomyocyte Metabolism

This study shows that adding macrophages to cardiomyocytes improves their metabolic maturity through mitochondrial clearance and mitophagy.

Beyond Ground Truth in K-Complex Detection: A Waveform-Based SVM Classifier and the Limits of Expert Agreement

The paper introduces an SVM classifier for detecting K-complexes in EEG data, achieving performance comparable to expert scorers and highlighting discrepancies in visual scoring.

Reciprocal macrophage-MSC crosstalk drives immunomodulatory and regenerative phenotypes in a mineralized collagen scaffold

This study shows that MSC-macrophage interactions in a mineralized collagen scaffold drive immunomodulatory and regenerative responses through IL-6 secretion and M2-like macrophage polarization.

Schwann Cell Mapping and Characterization in Bone of Different Embryonic Origins

This study maps and characterizes Schwann cells in bone, revealing differences in maturity and distribution between neural crest- and mesoderm-derived bone compartments.

Mechanisms of surface and volume light scattering from Caenorhabditis elegans revealed by angle-resolved measurements

This study investigates light scattering mechanisms in C. elegans using angle-resolved measurements and simulations, identifying surface and volume scattering components and their impact on image contrast.

Nuclear confinement from matrix stiffness drives epigenomic reprogramming of gingival fibroblasts

This study shows that matrix stiffness influences epigenomic changes in gingival fibroblasts, with stiff matrices promoting CpG methylation and reduced inflammation, while soft matrices lead to hypomethylation and increased inflammatory signaling.

A Sandwich-Structured Silk Fibroin Mesh with ROS-Responsive and Immunoregulatory Functions for Pelvic Floor Repair

This paper develops a ROS-responsive silk fibroin mesh with immunoregulatory properties for pelvic floor repair, showing improved immune modulation and tissue integration.

NEO-AAV: an engineered extracellular vesicle-enveloped AAV platform for activation-coupled T cell transduction and CAR-T cell generation

This paper introduces NEO-AAV, an engineered AAV platform that enhances T cell transduction and CAR-T cell generation by enveloping AAV in extracellular vesicles, improving immune resistance and transduction efficiency.

Integrative Multiomic Analysis Reveals How Non-Viral Delivery System Selection Shapes CRISPR Gene Editing Outcomes in Stem Cells.

This study compares non-viral CRISPR delivery methods in stem cells, finding that lipid-based delivery offers high efficiency but risks genomic instability, while CPPs reduce side effects at lower efficiency.

Information-Theoretic Functional Connectivity Characterizes Multiscale Network Reorganization in Postoperative Cognitive Decline

This study identifies network reorganization patterns in postoperative cognitive decline using static and dynamic connectivity measures from resting-state fMRI.