C. R. Bertozzi

Tumor Immune Cell Targeting Chimeras (TICTACs) reprogram tumor-associated macrophages

The paper develops TICTACs, which selectively target and degrade checkpoint receptors on tumor-associated macrophages without affecting other cells.

Microglia mediate contact-independent neuronal pruning via secreted Neuraminidase-3 associated with extracellular vesicles

This study shows that microglia secrete Neuraminidase-3 via extracellular vesicles to prune neurons contact-independently, disrupting network connectivity and contributing to neuroinflammatory diseases.

Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE

The paper uses a mucinase to analyze glycoproteomic features of TIM family proteins and shows how glycosylation affects their structure through molecular dynamics simulations.

Antibody-lectin chimeras for glyco-immune checkpoint blockade

This paper introduces antibody-lectin chimeras (AbLecs) as a new immunotherapy approach that blocks glyco-immune checkpoints, enhancing cancer immune responses and synergizing with existing checkpoint inhibitors.

Mutational screens highlight glycosylation as a modulator of CSF3R activity

The paper identifies glycosylation sites as critical for CSF3R activity and shows that mutations at these sites can lead to ligand-independent cell growth in chronic neutrophilic leukemia.

Comprehensive analysis of platelet glycoprotein Ibα glycosylation.

This study identifies O- and N-glycosites on GPIb and characterizes its glycan structures using mass spectrometry.

Design of a mucin-selective protease for targeted degradation of cancer-associated mucins

This paper designs a mucin-selective protease conjugated to a nanobody to degrade cancer-associated mucins, showing reduced tumor growth in mouse models.

Ferroptosis Regulation by the NGLY1/NFE2L1 Pathway

This study identifies the NGLY1/NFE2L1 pathway as a regulator of ferroptosis, showing that NGLY1-mediated deglycosylation of NFE2L1 is necessary for its anti-ferroptotic function through GPX4 expression.

Multi-Omics Analysis of Fibroblasts from the Invasive Tumor Edge Reveals that Tumor-Stroma Crosstalk Induces O-glycosylation of the CDK4-pRB Axis

The paper analyzes multi-omics data from fibroblasts at the tumor edge and core to show O-glycosylation of the CDK4-pRB axis is induced by tumor-stroma crosstalk.

Targeting cancer glycosylation repolarizes tumor-associated macrophages allowing effective immune checkpoint blockade

This study shows that targeting cancer glycosylation with an antibody-sialidase conjugate repolarizes tumor-associated macrophages and enhances immune checkpoint blockade efficacy in mouse models.

Anti-GD2 antibody disrupts GD2:Siglec-7 interactions and synergizes with CD47 blockade to mediate tumor eradication

This study shows that combining anti-GD2 and anti-CD47 antibodies enhances anti-tumor responses by recruiting macrophages and disrupting the GD2:Siglec-7 interaction in GD2+ cancers.

Revealing the human mucinome

This study develops a mucinomics platform using an inactive mucinase to enrich and identify mucin-domain glycoproteins, revealing molecular signatures in cancer samples.

On-tissue spatially-resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape

This paper develops a spatially-resolved glycoproteomics approach combining MALDI imaging and LC-MS/MS to identify glycan patterns in canine gliomas, revealing dysregulated glycosylation in tumor and necrotic regions.

Cell type-selective secretome profiling in vivo

The paper introduces a method to profile cell-type-specific secretomes in vivo and identifies novel secreted proteins and dynamic changes in hepatocyte secretomes.

Towards Mycobacterium tuberculosis detection at the point-of-care: a brighter solvatochromic probe permits the detection of mycobacteria within minutes

This paper develops a solvatochromic dye conjugate for rapid, sensitive detection of Mycobacterium tuberculosis in minutes.

Systematic identification of regulators of antibody-drug conjugate toxicity using CRISPR-Cas9 screens

This study uses CRISPR-Cas9 screens to identify genes regulating antibody-drug conjugate (ADC) toxicity, revealing endolysosomal trafficking regulators and mechanisms of ADC resistance.

Detection of live mycobacteria with a solvatochromic trehalose probe for point-of-care tuberculosis diagnosis

This paper develops a fluorogenic trehalose analog to detect live mycobacteria, showing it can label Mtb in clinical samples and is reduced by TB drugs.

A Bulky Glycocalyx Fosters Metastasis Formation by Promoting G1 Cell Cycle Progression

This study shows that a bulky glycocalyx promotes metastasis by enabling G1 cell cycle progression through integrin adhesion and signaling.