Shane Crotty

Germinal centers can persist for up to one year with declining somatic hypermutation and ongoing plasma cell output

This study tracks germinal center responses in rhesus macaques over a year, showing prolonged GCs with declining SHM and ongoing plasma cell production.

Durability and diversity of human upper airway T cell memory

This study longitudinally tracks antigen-specific T cells in human upper airway tissues and blood, finding sustained memory T cell populations over 18 months.

Heterologous betacoronavirus spike immunization in nonhuman primates elicits cross-reactive antibodies that neutralize both sarbeco- and merbecoviruses

This study vaccinates nonhuman primates with SARS-CoV-2 and MERS-CoV spike proteins to elicit cross-reactive antibodies that neutralize both sarbecoviruses and merbecoviruses.

Immunofocusing on the conserved fusion peptide of HIV envelope glycoprotein in rhesus macaques

This study shows that targeting the conserved HIV fusion peptide with modified Env immunogens in rhesus macaques can focus immune responses on neutralizing epitopes.

Vaccination with mRNA-encoded membrane-bound HIV Envelope trimer induces neutralizing antibodies in animal models

Membrane-bound BG505 MD39.3 Env trimer delivered as mRNA-LNP reduced base-directed antibody responses in rabbits and macaques and elicited neutralizing antibodies, with memory B cell responses documented in macaques.

Local B-cell immunity and durable memory following live-attenuated influenza intranasal vaccination of humans

This study shows that intranasal influenza vaccination generates durable local B cell memory in the upper airway, while intramuscular vaccination does not.

Evolution of SARS-CoV-2 T cell responses as a function of multiple COVID-19 boosters

This study examines T cell and antibody responses in vaccinated individuals over three years, finding stable T cell responses and increased regulatory T cells after multiple boosters.

Virus glycoprotein nanodisc platform for vaccine design

The paper presents a nanodisc platform for assembling transmembrane glycoproteins into vaccine candidates, demonstrating its utility in HIV vaccine development through structural and functional analyses.

Diverse priming outcomes under conditions of very rare precursor B cells

The paper investigates how vaccine antigen delivery and adjuvant selection affect the priming of rare HIV broadly neutralizing antibody-precursor B cells in non-human primates, finding that specific dosing and adjuvant combinations enhance their detection in germinal centers.

Dose-dependent regulation of immune memory responses against HIV by saponin monophosphoryl lipid A nanoparticle adjuvant

This study evaluates the dose-dependent effects of the SMNP adjuvant on HIV Env-specific immune responses in non-human primates, showing increased memory T and B cell responses and neutralizing antibodies at higher doses.

A Broad Survey and Functional Analysis of Immunoglobulin Loci Variation in Rhesus Macaques

The paper develops methodologies to generate and analyze immunoglobulin loci variation in rhesus macaques, revealing extensive diversity and expanding existing allele sets by 60%.

Profiling Transcription Initiation in Peripheral Leukocytes Reveals Severity-Associated Cis-Regulatory Elements in Critical COVID-19

The paper profiles the active cistrome in peripheral leukocytes of critically ill COVID-19 patients to identify severity-associated cis-regulatory elements and their regulatory programs.

AI-guided discovery of the invariant host response to viral pandemics

The paper identifies a conserved 166-gene host immune signature across viral pandemics, with a 20-gene subset predicting disease severity and guiding therapeutic strategies.