Hematopoietic Stem Cell Transplantation

This paper shows that CD201 is a more reliable marker than SCA1 for identifying long-term reconstituting hematopoietic stem cells in mice after septic challenge.

The paper shows that mitochondrial content influences lineage commitment in megakaryocyte-erythroid progenitors, with mitochondria-rich cells favoring erythroid differentiation and mitochondria-poor cells favoring megakaryocyte differentiation.

This study shows that erythroid precursors in the bone marrow increase local oxygen levels after injury, promoting tissue repair by enhancing angiogenesis and osteogenesis.

The paper investigates the roles of Atf3, Zfp711, and Bcl6b in early hemato-endothelial lineage specification using scRNA-seq and KO mESCs, finding distinct effects on mesodermal and hematopoietic lineages.

The paper identifies a mesodermal progenitor in mouse gastrula and human iPSC cultures that gives rise to pro-definitive angio-hematopoietic lineage cells, including yolk sac endothelium, blood islands, and macrophages.

The paper creates a spatial atlas of human gastrointestinal GVHD, identifying immune and epithelial changes that contribute to disease pathophysiology.

The paper develops a single-cell transcriptomic map of cynomolgus macaque hematopoietic stem cells and progenitors, revealing molecular differences from humans and a direct differentiation pathway to megakaryocyte lineages.

This study shows that linker histone H1.0 regulates HSPC fate bias toward lymphoid or myeloid differentiation, with H1.0 levels decreasing in response to IFN signaling.

This study identifies desaminotyrosine (DAT) as a microbial metabolite that protects against graft-versus-host disease by promoting intestinal regeneration and modulating immune responses.

This study identifies GABA as a regulator of HSPCs produced by B cells and endothelial cells in the bone marrow, showing lower GABA levels reduce proliferation and increase B cell differentiation.

This study optimizes the dose and timing of MSC-derived culture-conditioned media (CCM) to enhance its immunomodulatory effects on CD3+ T-cells in acute graft-versus-host disease.

The paper identifies two bone marrow compartments and shows inflammation affects them differently, with type I interferon causing changes in mesenchymal stromal cells.

This paper develops a streptavidin-drug conjugate platform to efficiently optimize antibody-based conditioning for HSCT, finding that PBD dimer SGD-1882 is most effective for targeting HSCs and AML cells.

This paper introduces a 3D hemogenic gastruloid model to study infant acute myeloid leukemia origins, showing MNX1-driven transformation of erythroid-myeloid progenitors.

This study identifies PF4 as a key regulator of HSC aging, showing that PF4 deficiency accelerates aging and that PF4 supplementation can rejuvenate aged HSCs.

This study identifies new functionally and molecularly distinct multipotent progenitors in adult human hematopoiesis using multiomic single cell analyses and functional assays.

The paper shows that myeloid-biased hematopoiesis in aging is due to altered LT-HSC/ST-HSC ratios, not HSC clone heterogeneity.

This study identifies distinct causes for three hematopoietic aging phenotypes using mouse models and single-cell analysis, showing HSC accumulation is NFκB-independent, functional impairment is HSC-autonomous NFκB-driven, and myeloid bias is driven by the NFκB-altered bone marrow environment.

This study shows that chronic glucocorticoid exposure, via HIF1 deficiency in adrenocortical cells, promotes HSPC expansion and myeloid bias while impairing B-cell development through GR-dependent mechanisms.

This study shows BMI1 promotes erythroid self-renewal through both gene repression and activation, offering a strategy to expand immature erythroid precursors for clinical use.

This paper shows that pretransplant targeting of TNFRSF25 and CD25 with a TL1A-Ig fusion protein and low-dose IL-2 expands recipient Tregs, reducing GVHD after HSCT while preserving GVL.

This study identifies how Gata2 haploinsufficiency disrupts HSC function and drives pre-leukemic changes through DNA damage and inflammatory signaling, with ASXL1 mutations exacerbating these defects.

The paper investigates lineage commitment bias in human and murine hematopoietic stem cells, finding that variation in myeloid and lymphoid engraftment is stochastic and not due to cell-intrinsic bias.

This paper develops a system to predict hematopoietic stem cell diversity using quantitative phase imaging and machine learning, capturing temporal kinetics rather than single snapshots.

This study evaluates the dose- and time-dependent effects of CoPP on granulocyte mobilization and metabolic markers in mice, finding a dose-dependent mobilizing effect with mild side effects.

This study identifies an epigenetically distinct HSC subset, Kitlo, that supports better thymic and T-cell reconstitution in mice and humans.

This paper develops a 3D artificial marrow organoid model to study NK and myeloid cell differentiation, showing improved immune receptor expression and drug response compared to 2D models.

The paper uses single-cell and spatial transcriptomics to study hematopoietic stem and progenitor cell diversity and their niches in zebrafish development.

This study examines the long-term effects of medium-dose irradiation and 5-FU on hematopoietic stem cells and hematopoietic recovery in mice, finding that irradiation causes sustained impairments and selective depletion of MHC class II- HSCs.

This study shows PERK signaling promotes HSC proliferation under ER stress to maintain stem cell pool integrity by activating mTOR, without relying on apoptosis.