Hematopoietic Stem Cell Transplantation

Modeling competitive transplantation using HLA-mismatched human hematopoietic stem cells

This paper introduces a competitive transplantation method using HLA-mismatched human hematopoietic stem cells in humanized mice to study repopulating capacity and track graft dynamics.

Transforming clinical apheresis waste into a renewable source of patient-derived CD34+ hematopoietic stem cell biobank for beta-hemoglobinopathy research and therapeutic discovery

This paper describes a method to recover and biobank CD34+ hematopoietic stem cells from clinical apheresis waste for hemoglobinopathy research, showing comparable yields and viability to peripheral blood samples.

Midbody inheritance predicts re-entry into quiescence, but not lineage potential, in mouse hematopoietic stem cells

This study shows midbody inheritance in HSCs delays re-entry into the next cell cycle but does not affect lineage potential.

GRIMM-II: A Two-Stage Real-Time Algorithm for Nine-Locus HLA Imputation and Matching with Up to Three Mismatches

GRIMM-II improves HLA donor matching by imputing nine loci and identifying donors with up to three mismatches in real-time.

Chromatin Accessibility Shapes Developmental-Specific Lineage Plasticity in Hematopoiesis

The paper shows fetal hematopoietic stem and progenitor cells (HSPCs) have greater lineage plasticity than adult HSPCs, linked to chromatin accessibility differences.

Resolving hematopoietic stem versus progenitor cell potential in the mouse dorsal aorta by differential Runx1 +110 enhancer activity

The paper identifies a Runx1 enhancer that distinguishes hematopoietic stem cells (HSCs) from progenitor cells (HPCs) in the mouse dorsal aorta using enhancer-reporter models and single-cell multiome analysis.

Clonal memory of cell division in humans diverges between healthy haematopoiesis and acute myeloid leukaemia

The paper shows clonal memory in human HSPCs persists across divisions and is disrupted in AML, with potential for therapeutic modulation.

JARID2 Inhibition Reprograms Human Hematopoietic Progenitor Cells To Enhance Bone Marrow Transplantation

JARID2 inhibition enhances repopulating activity of human hematopoietic stem and progenitor cells in vivo via STAT1 upregulation.

Donor-Targeted Anti-HLA-A2 Antibody Shows Nanogram-Level Efficacy in a Novel GVHD Mouse Model

This paper develops an HLA-A2-specific antibody that effectively reduces GVHD in a mouse model with minimal dose.

Septin7 is essential in early hematopoiesis, but redundant at later stages

This study shows Septin7 is essential for early hematopoiesis but dispensable at later stages, as its deletion in hematopoietic cells leads to engraftment failure but not steady-state blood lineage defects.

Niche-targeted therapy via YAP/TAZ activation enhances hematopoietic regeneration

This study shows that activating YAP/TAZ improves bone marrow niche recovery and hematopoietic regeneration after myeloablative stress.

Quantitative molecular cartography of emergency myelopoiesis reveals conserved modules of hematopoietic activation

The paper identifies shared and distinct molecular mechanisms of emergency myelopoiesis using scRNA-seq and develops tools to annotate and model hematopoietic differentiation.

Activating an Interleukin-4-FLT3-STAT6 axis in Multipotent Progenitors Restores Lymphopoiesis in Inflammation and Aging

This study identifies IL-4 as a signal that restores lymphopoiesis in inflammation and aging by activating the FLT3-STAT6 pathway in multipotent progenitors.

Ex Vivo Expansion of Hematopoietic Stem and Progenitor Cells from Human Mobilized Peripheral Blood for Gene Therapy Applications

This study optimizes an ex vivo expansion protocol for mobilized peripheral blood HSCs, enabling robust gene therapy applications with long-term engraftment.

A kinetics-based model of hematopoiesis reveals extrinsic regulation of skewed lineage output from stem cells

This study uses single-cell transplantation and mathematical modeling to show that lineage biases in hematopoiesis arise from extrinsic feedback and clonal competition, not intrinsic HSC properties.

Local Oxygen Tension Dictates Hematopoietic Cell Growth and Potency

This study shows that local oxygen levels affect hematopoietic cell growth, metabolism, and potency, with low oxygen preserving less proliferative, stress-resistant cells.

MLL3 and MLL4 sustain hematopoietic stem cell multipotency by opposing a B-cell default state

This study shows that MLL3 and MLL4 maintain hematopoietic stem cell multipotency by opposing a B-cell default state through distinct enhancer regulation mechanisms.

Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrow

The paper identifies adult hemogenic endothelial cells in mouse bone marrow that generate hematopoietic stem/progenitor cells and functional blood cells.

Imlifidase and EndoS enables semi-allogeneic bone marrow engraftment in sensitized mice under reduced intensity conditioning

The paper shows that imlifidase and EndoS desensitization allows long-term bone marrow engraftment in sensitized mice with reduced intensity conditioning.

Corticosteroid resistance is predetermined by early immune response dynamics at acute Graft Versus Host disease onset

The paper identifies intrinsic immune cell mechanisms that drive steroid resistance in acute graft versus host disease before treatment.

Long-term clonal analysis using stochastic models reveals heterogeneity and quiescence of hematopoietic stem cells

The paper uses stochastic models to analyze clonal data from non-human primates, showing that HSC heterogeneity and quiescence are critical for long-term hematopoiesis.

Single Spectral Flow Cytometry Panel for Simultaneous Detection of Hematopoietic Stem, Progenitor, and Mature Lineages in Mouse Bone Marrow

The paper introduces a 19-marker spectral flow cytometry panel to simultaneously identify hematopoietic stem, progenitor, and mature cell lineages in mouse bone marrow.

Advanced Deep Learning Enables Prediction of Allogeneic Stem Cell Mobilization Success

This paper develops machine learning models to predict donor success in stem cell mobilization using clinical data, enabling early intervention and personalized strategies.

Bnip3lb-driven mitophagy sustains expansion of the embryonic hematopoietic stem cell pool

This study shows that Bnip3lb-driven mitophagy maintains embryonic HSPC multipotency and expansion by regulating ROS levels, and enhances ex vivo hematopoietic potential of iPSC-derived HSPCs.

Proliferative events ameliorate DNA damage accumulation without affecting function in hematopoietic stem cells

This study shows that increased HSC proliferation reduces age-related DNA damage without affecting function in mice.

An Inflammatory and Quiescent HSC Subpopulation Expands with Age in Humans

The paper identifies an age-related expansion of an inflammatory and quiescent HSC subpopulation using integrated single-cell data.

Novel markers for haemogenic endothelium and haematopoietic progenitors in the mouse yolk sac

The paper identifies novel molecular markers for haemogenic endothelium and haematopoietic progenitors in mouse yolk sac using single-cell RNA sequencing and pseudotime analysis.

Serum-free differentiation platform for the generation of B lymphocytes and natural killer cells from human CD34+ cord blood progenitors

This paper introduces a serum-free co-culture system using human bone marrow stromal cells to differentiate cord blood CD34+ progenitors into B and NK cells, offering a more physiologically relevant model for hematopoiesis research.

Murine limb ischaemia induces structural and functional remodelling of local and distant bone marrow

This study examines how murine limb ischaemia affects bone marrow structure and function, revealing both local and systemic changes in haematopoiesis and myeloid cell function.

Endothelial protein C receptor CD201 is a better marker than SCA1 to identify mouse long-term reconstituting hematopoietic stem cells following septic challenge

This paper shows that CD201 is a more reliable marker than SCA1 for identifying long-term reconstituting hematopoietic stem cells in mice after septic challenge.