Lipid Membrane Structure and Behavior

Protein-protein interaction networks can be highly sensitive to the membrane phase transition

The paper models how membrane phase transitions affect protein-protein interaction networks, showing sensitivity to thermodynamic parameters near critical points.

Unconventional Fusion Mechanism at the Origin of Eukaryotic Membranes

This study proposes a non-canonical lipid-mediated mechanism for eukaryotic membrane formation through molecular dynamics simulations.

DNA Framework Nanoreactor for Programmable Membrane Fusion

This paper introduces a DNA framework vesicle nanoreactor that enables programmable and quantifiable membrane fusion by controlling geometry and kinetics.

QuantGUV: Quantifying Encapsulation Efficiency of Small Molecules in GUVs

QuantGUV measures encapsulation efficiency of small molecules in GUVs using image-based analysis, revealing that efficiency varies with experimental conditions.

Membrane curvature during membrane rupture and formation of pentagonal pyramidal superassemblies by a pore-forming toxin, Vibrio cholerae Cytolysin, using single particle cryo-EM

This study uses cryo-EM to show how Vibrio cholerae Cytolysin induces membrane curvature and forms superassemblies during membrane rupture.

Dynamic shape remodeling of vesicles by internal active filaments

This study uses simulations to show how active filaments inside vesicles drive dynamic shape changes under osmotic stress, forming unstable structures like branched tubes and compartmentalized vesicles.

In situ evidence for systematic membrane thickness variation across cellular organelles

The paper develops a computational method to measure membrane thickness in cryo-electron tomograms, revealing systematic variations across organelles and supporting hydrophobic matching models.

Direct measurement of PIP2 densities in biological membranes using a peptide-based sensor

The paper develops a peptide-based sensor to measure PI(4,5)P2 densities in membranes, showing mutation-specific changes in surface levels in cells with Ras mutations.

Lipid packing and local geometry influence septin curvature sensing

This study shows that lipid packing and membrane geometry jointly regulate septin localization in vitro and in cells.

Homeostatic regulation of intrinsic lipid curvature in eukaryotic cells

This study shows eukaryotic cells maintain lipid curvature homeostasis under pressure through metabolic adjustments, with yeast and human cells using different mechanisms.

Lipid tail chemistry regulates selective membrane interactions with model DNA nanoprobes and DNA-based coacervates

This study investigates how lipid acyl-chain chemistry affects membrane properties and interactions with DNA nanoprobes and coacervates, identifying a packing-dependent guiding principle for membrane interactions.

Dynamic Shape Modulation of Deflated and Adhered Lipid Vesicles

This study shows that weakly adhered giant unilamellar vesicles can be deflated to small volumes, and uses shape analysis to determine mechanical parameters related to membrane behavior.

Palmitoylation of PSD95 flips the bilayer juxtaposed domain and drives cluster formation on membrane

This study reveals how palmitoylation of PSD95 reorients its domains to promote membrane association and clustering, with lipid composition modulating this process.

Unveiling Eukaryotic Membrane Proteins in High Resolution Using Peptide Solubilization

This paper introduces a peptide-based method to solubilize eukaryotic membrane proteins without detergents, enabling high-resolution structural studies.

Lateral lipid packing governs bilayer solubilization by styrene-maleic acid copolymers: a case study with cardiolipin-containing membranes

This study investigates how cardiolipin affects SMA copolymer-mediated membrane solubilization by examining lateral lipid packing effects.

Packing of apolar amino acids is not a strong stabilizing force in transmembrane helix dimerization.

The paper designs and tests transmembrane helix dimers to assess if apolar side chain packing and van der Waals interactions are sufficient for stabilizing dimerization, finding they are weak unless highly optimized.

The longer transmembrane helices of class I viral fusion proteins may facilitate viral fusion

The paper examines the lengths of transmembrane helices in class I viral fusion proteins and finds they are longer than model membranes, suggesting functional relevance for viral fusion.

Conformational changes, excess area, and elasticity of the Piezo protein-membrane nanodome from coarse-grained and atomistic simulations

This study uses simulations to analyze the conformational changes and elasticity of Piezo proteins in membrane nanodomains under tension, finding a half-maximal reduction of excess area at 3-4 mN/m tension.

The action mechanism of actinoporins revealed through the structure of pore-forming intermediates

The paper determines the structures of two actinoporins in a membrane environment using cryogenic electron microscopy, revealing their pore-forming mechanism through intermediate states.

Tryptophan-aspartic acid containing peptide analog from coronin 1 inhibits model membrane fusion and enveloped viral infection in cells

The paper designs a tryptophan-aspartic acid peptide analog, mGG21, that inhibits membrane fusion and enveloped viral infection by altering membrane physical properties.

Leaflet-specific phospholipid imaging using genetically encoded proximity sensors

This paper introduces FACES, a tool for imaging lipid pools and transbilayer orientation in living cells, and applies it to study lipid transfer proteins and membrane asymmetry.

Micron-scale, liquid-liquid phase separation in ternary lipid membranes containing DPPE

This study investigates how phosphatidylethanolamine (PE) lipids affect liquid-liquid phase separation in model membranes, finding that PE lipids suppress phase separation compared to phosphatidylcholine (PC) lipids.

Thermotropic Properties of Large, Circularized Nanodiscs

This study examines how the size of circularized nanodiscs affects lipid bilayer properties, finding that larger nanodiscs better approximate lipid behavior in large unilamellar vesicles.

Nanoscale PI(4,5)P2 partitioning driven by FGF2 oligomerization triggers membrane pore formation

This paper shows FGF2 oligomerization drives PI(4,5)P2 partitioning and membrane pore formation, enabling FGF2 translocation across the plasma membrane.

DNA Flipping as Facile Mechanism for Transmembrane Signaling in Synthetic Cells

This paper demonstrates DNA flipping across lipid membranes in synthetic cells to enable transmembrane signaling and transcriptional activation.

SARS-CoV E protein couples asymmetric leaflet thickness and curvature deformations

This study shows SARS-CoV E proteins cause local membrane deformations and asymmetry but not long-range curvature, highlighting the role of hydrophobic mismatch and membrane asymmetry in deformation energy.

Membrane Proteins at Scale: Automated Copolymer Nanodisc Purification for Structure and Function

This paper introduces an automated copolymer screening platform for membrane protein solubilization, showing next-generation copolymers outperform classical ones across multiple human targets.

TS2CG as a membrane builder

The paper introduces TS2CG version 2, a tool for building coarse-grained membrane structures with specific shapes and lateral organization, demonstrating its capabilities through various simulations.

Structural Role of Stomatin in Organizing Functional Membrane Microdomains

This study determines the cryo-EM structure of stomatin, revealing its role in organizing functional membrane microdomains and identifying associated proteins involved in cell migration and vesicle trafficking.

Atomistic modelling of lysophospholipids from the Campylobacter jejuni lipidome

The paper presents atomistic models of lysophospholipids from Campylobacter jejuni and evaluates their phase behavior and membrane properties using molecular dynamics simulations.