Cellular transport and secretion

This study uses molecular dynamics simulations to show that Stx1A TMD palmitoylation stabilizes its conformation, facilitates SNARE complex formation, and modulates fusion pore dynamics during neurotransmitter release.

PDGFR/{beta} heterodimer binding to MYO1D promotes rapid internalization, inhibiting ERK1/2 phosphorylation and reducing cell proliferation.

The paper characterizes intra-Golgi trafficking intermediates by analyzing proteomic profiles of Golgi-derived vesicles under COG complex dysfunction, revealing COG-dependent recycling of glycosylation machinery.

This study investigates the role of FLWR-1 in synaptic vesicle recycling and neuronal activity in C. elegans, finding it regulates Ca2+ levels via PMCA and affects excitation-inhibition balance.

The paper identifies a novel cellular organelle complex called hemifusomes through cryo-electron tomography, revealing their role in intraluminal vesicle biogenesis.

The paper identifies an unconventional Pex5-Eci1 interaction interface that enables PTS1-independent peroxisomal import of Eci1 in yeast.

The paper identifies two CLC binding sites on Sla2 and shows Pan1 competes with CLC for a conserved site, revealing divergence in endocytic mechanisms between fungi and metazoa.

This study identifies VPS35/retromer as a regulator of MT1-MMP localization and stability, showing its role in melanoma metastasis and suggesting it as a therapeutic target.

The paper shows that cargo adaptors bind myosin V through multiple sites, using a handhold mechanism to enhance motor-cargo interactions and regulation.

This study identifies a novel pathway involving retromer and trans-Golgi SNARE proteins that regulates apoplastic effector secretion in Magnaporthe oryzae, essential for pathogenicity.

The paper shows that TFG forms a hollow condensate at the ER-Golgi interface, acting as a molecular sieve that separates anterograde and retrograde carriers.

The paper profiles the LAM family of contact site tethers to understand their localization and function, revealing unique and shared roles through proximity labeling.

This study investigates how Vps5 and Vps17 SNX-BAR proteins assemble with Retromer, revealing a PL motif in Vps5 that binds Vps29 and is essential for membrane tubule association and cargo recycling.

This study shows ATP9A and ATP9B form complexes and are involved in transporting VSVG from the Golgi to the plasma membrane in the secretory pathway.

This study identifies a mechanism by which UNC-13 in C. elegans regulates synaptic vesicle exocytosis through domain interactions and functional switches.

The paper shows that VPS13C senses lysosome damage, relocates to lysosomes, and tethers them to the ER, suggesting a protective lipid transport response.

The paper tracks exocytic vesicle movements in epithelial cells to show microtubule organisation controls apical secretion and reveals spatially organised ECM secretion.

This study shows that lysosomal positioning in melanoma cells correlates with aggressiveness, promoting invasion and matrix degradation.

The paper uses a SNAP25-based FRET construct to show SNARE complex assembly and disassembly dynamics in live cells, revealing that FRET ratio transients reflect complex disassembly rather than diffusion.

This study shows that HOPS/CORVET complexes are essential for malaria parasite endocytosis and invasion organelle function, with distinct roles in different developmental stages.

This study investigates the role of BECLIN-1 in maintaining gastrointestinal epithelial integrity by regulating endocytic trafficking, F-actin organization, and lysosomal function using organoids from BECLIN-1 knockout mice.

This study shows Rab10 maintains Ca2+ homeostasis and protein synthesis to regulate neuropeptide release via DCVs in mouse hippocampal neurons.

This study shows RalB uncouples from the exocyst to mediate Weibel-Palade body exocytosis in endothelial cells through phosphorylation-dependent regulation.

This study determines the structure of the AP5:SPG11-SPG15 complex and shows it binds PI3P, senses membrane curvature, and drives membrane remodeling in vitro.

This study shows Salmonella Typhimurium manipulates syntaxin 7 to alter endo-lysosomal trafficking, aiding its survival in host cells.

This study identifies phosphorylation sites in Aly3 that regulate Ght5 endocytosis and proliferation in low glucose in fission yeast.

The paper quantifies evolutionary fitness gains of endomembrane systems through two models: pinocytosis and proto-endoplasmic reticulum, finding the latter provides net fitness gains.

This study models membrane deformation during endocytosis in yeast, showing that boundary lipid flow under turgor pressure drives tubulation, independent of actin polymerization.

The paper identifies LyLAP as a key enzyme for lysosomal degradation of transmembrane proteins, showing its role in maintaining endocytic activity and lysosomal stability in pancreatic cancer.

The paper determines the structure of ARF6 protein lattice on tubulated membranes, revealing its role in vesicle fission and how GAP proteins interact with it.