microscopy & imaging analysis

The paper reports the cryo-EM structures of an Acinetobacter baumannii podovirus, revealing its capsid maturation mechanism and providing a reference for phage engineering.

This study investigates how tau protein regulates motor proteins on microtubules using single-molecule imaging, cryo-EM, and MD simulations, revealing distinct mechanisms for inhibiting kinesin-1 and dynein.

The paper maps AMPA and NMDA receptors at mouse hippocampal synapses using cryo-ET, revealing their distribution and relationship to glutamate release sites.

This study determines the structure of human GluD1 and elucidates its ligand-gating mechanism using cryoEM and electrophysiology.

The paper determines the Cryo-EM structure of the Arabidopsis thaliana V-type ATPase, revealing its catalytic cycle and organelle-specific interactions with TLDc proteins.

This study uses cryo-EM to reveal how calcium binding to connexin-46/50 gap junctions induces structural changes leading to pore collapse and gating.

This study identifies structural adaptability in HIV-2 Vif that enables broad antagonism of APOBEC3G through conformational selection.

This study determines the cryo-EM structure of HIV-1 capsid lattice with lenacapavir, showing how it disrupts capsid formation and function during virion maturation.

This study uses cryo-EM and tomography to reveal the structural changes in bacteriophage P2 during tail contraction and host infection, identifying new architectural features and mechanisms of genome translocation.

This study compares low-magnification super-resolution and physical-pixel workflows in cryo-EM, showing that effectiveness depends on specimen-specific signal and sampling limitations.

This study uses cryogenic electron tomography to visualize the mechanism of membrane perforation during rhesus rotavirus entry, linking structural data with live-cell imaging.

The paper determines the in vivo structures of P22 infection intermediates and explains how gp10 templates the sequential assembly of three proteins into a trans-envelope channel for genome translocation.

This study presents cryo-EM structures of MBOAT2, revealing its catalytic mechanism, product retention, and ligand exchange dynamics.

The paper resolves the native structure of the Trichonympha centriole cartwheel using cryo-electron tomography, revealing a zigzag stacking pattern of SAS-6 tetramers and their role in structural stability and polarity.

The paper investigates the structural organization and assembly of the bacterial spore envelope in Clostridium sporogenes, revealing common principles of crystalline and disulphide-based self-assembly across distantly related bacteria.

The paper reports the cryoEM structure of the ITG3β1-CD151 complex and shows CD151 promotes ITG3β1 surface export, explaining the epidermolysis bullosa phenotype.

This paper introduces a method to reduce air-water interface artifacts in cryo-EM by encapsulating proteins in hydrophilic shells, demonstrating its effectiveness with three protein examples.

This study identifies lipoprotein-mediated endocytosis as a conserved mechanism for cationic cell-penetrating peptides across animal lineages, showing it modulates their antimicrobial function.

This study determines cryo-EM structures of orthoflaviviruses bound to LDLR family receptors, revealing distinct binding modes and receptor engagement mechanisms.

This study investigates the assembly and conformational dynamics of insect odorant receptor complexes using high-speed atomic force microscopy and cryo-EM, revealing non-tetrameric oligomeric states and symmetry-broken configurations.

This study reveals that INO80 reorients on a nucleosome upon ATP hydrolysis, enabling DNA translocation through a unique mechanism distinct from other chromatin remodelers.

The paper compares RSV and hMPV entry strategies, showing RSV uses structural organization for thermotolerance while hMPV uses genomic redundancy for stability.

This study uses cryo-EM and charge detection mass spectrometry to determine the stoichiometry and structure of Bpa-substrate complexes in Mtb, revealing an undecameric apo form and a Bpa12:HspR2 complex.

This study identifies a chain-feeding mechanism in BRCA1-A that explains its preference for long K63-linked ubiquitin chains through avidity and processivity.

This paper determines the cryo-EM structure of the PI3KC3-C2 complex with Rubicon's PIKBD domain, revealing how Rubicon selectively inhibits PI3KC3-C2 to regulate endolysosome maturation and mitophagy.

This study reports cryo-EM ribosome structures and rRNA modifications in Babesia divergens, revealing novel modifications with potential therapeutic implications.

The paper identifies an essential Ndc10 trimerization interaction in yeast kinetochores that stabilizes centromeric DNA and facilitates kinetochore assembly.

This study investigates BCoV spike protein evolution, showing preserved architecture but functional region remodeling, with implications for vaccine development.