wet-lab / biochemical assay

This study identifies WRNIP1's role in preventing DNA damage caused by G4/R-loop-associated transcription-replication conflicts by stabilizing FANCJ at G4 sites.

This study shows 5-ALA labeling is not specific to transformed glioma cells but can label untransformed cells directly or via PpIX from surrounding tumor cells.

This study shows that cleavage of cFLIP by Caspase-8 limits cell death during viral infection and tissue injury, promoting tissue repair.

The paper identifies NMNAT2 as a collateral lethality target in cancers with NMNAT1 deletions, showing selective toxicity in vitro and tumor eradication in vivo.

This study evaluates rapamycin's specificity as an mTOR inhibitor using gene-edited cells, showing minimal off-target effects on transcriptome and proteome.

The paper designs and evaluates 2,7,9-trisubstituted purin-8-ones as FLT3-ITD inhibitors, identifying compound 15a with improved selectivity and activity in vitro and in vivo.

The paper identifies LUBAC-mediated M1 ubiquitination as a species-specific checkpoint regulating necroptosis by controlling MLKL membrane accumulation in human cells and organoids.

The paper identifies a cell surface-triggered auxin signaling mechanism that regulates PIN1-mediated auxin efflux and developmental patterning in Arabidopsis.

This study identifies ARF6 as a critical vulnerability in PTEN-deficient ovarian cancer, showing its role in integrin-dependent invasion and its inverse association with patient outcomes.

This study shows that non-redundant DMSO reductases in S. Typhimurium enhance anaerobic growth and virulence by supporting electron acceptor function and type-3 secretion systems.

MuVEH and mitoMuVEH enhance variant detection in scRNA-seq and enable clonal relationship reconstruction in AML patients.

This study evaluates the neutralizing antibody resistance of SARS-CoV-2 Omicron subvariants BA.2.75.2, BA.4.6, and BQ.1.1 to monoclonal antibodies and sera from vaccinated individuals.

This study shows therapy-induced senescence in AML upregulates antigen presentation and enhances T-cell anti-leukemic activity, suggesting senescence as a target for immunotherapy.

The paper develops a method to construct genome-wide libraries for yeast surface display and identifies drug targets in protozoan pathogens using a fitness screen.

The paper maps immunocyte migration from the gut using single-cell genomics and a photoconvertible mouse model, showing lineage-specific emigration patterns and their influence on peripheral immunity.

This study identifies SAM68 as a regulator of endothelial cell adhesion and basement membrane assembly through RNA localization and transcriptional modulation.

This study shows lysosomes mediate the mitochondrial UPR through mTORC1-dependent ATF4 phosphorylation, essential for mitochondrial redox homeostasis and cell survival under stress.

The paper investigates the role of Calaxin in stabilizing outer arm dynein docking onto ciliary microtubules in vertebrates using zebrafish and structural analysis.

This study identifies PiC2 as a copper transporter regulated by MTF1, essential for skeletal muscle myoblast proliferation and differentiation by delivering copper to COX.

The paper introduces RAD-TGTs, a method to measure cellular tensions using DNA duplexes that rupture under force, with readout via flow cytometry and DNA sequencing.

The paper examines CD8+ T cell responses in human lymph nodes during cancer immunotherapy and finds that metastasis disrupts these responses.

The paper evaluates HIV-1 vaccine candidates using HCDR3-engineered B cells, finding that SOSIP-TM mRNA-lipid nanoparticles induce stronger anti-apex responses than multimerized SOSIP proteins.

The paper shows malonyl-CoA inhibits mTORC1 by competing with ATP, linking lipid biosynthesis to cellular growth regulation in yeast and mammals.

The paper identifies UBR5 as a quality control enzyme that degrades unpaired transcription factor subunits, shaping network dynamics and gene expression through continuous turnover.

This paper develops a therapeutic vaccine using tumor-derived extracellular vesicles modified with polymeric surfactants to enhance immunogenicity and efficacy against melanoma.

This study identifies SNX32's role in cargo sorting via its PX and BAR domains, showing its involvement in transferrin trafficking and neuronal differentiation.

The paper examines how S. mansoni infection impacts HIV-specific T-cell responses in coinfected Ugandan fishermen, finding reduced immune responses after parasite clearance.

This study shows that inhibiting the BMP pathway suppresses colorectal cancer growth by reducing EGFR, and combining BMP and MEK inhibition enhances therapeutic effects in patient-derived organoids.

This study shows that the intrinsically disordered region of Fus1 mediates condensation of the actin fusion focus, essential for yeast gamete fusion and preventing cell lysis.

This study identifies 4-hydroxynonenal as the ligand for GDAP1, showing its binding requires a unique -loop and that mutations affecting this loop impair 4HNE binding and contribute to CMT disease.