flow cytometry

Survival and Developmental Progression of Unselected Thymocytes in the Absence of the T Cell Adaptor Gads

This study investigates the role of Gads in thymocyte development by ablating Gads and tracking thymocyte progression, finding that Gads is essential for TCR-driven checkpoints and death by neglect of unselected cells.

Single-cell transcriptomic analysis of NK cell dynamics in myeloma patients reveal persistent reduction of cytotoxic NK cells from diagnosis to relapse

Flow cytometric isolation of drug-like conformational antibodies specific for amyloid fibrils

This paper describes a FACS method using quantum dots to isolate high-quality conformational antibodies against insoluble antigens like amyloid fibrils.

An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancer

This paper develops an ICG-based liquid biopsy test to detect circulating tumor cells in pediatric liver cancer patients, showing that CTC burden correlates with therapy response.

Quantification of Aquatic Unicellular Diazotrophs by Immunolabeled Flow Cytometry

This study develops a flow cytometry-based method to quantify unicellular diazotrophs by immunolabeling nitrogenase, revealing their abundance and nitrogen fixation rates in marine and freshwater environments.

Autoantibody subclass predominance is not driven by aberrant class switching or impaired B cell development

The paper investigates why IgG4 autoantibodies predominate in certain autoimmune diseases and finds that B cell development is normal, suggesting antigen-driven subclass predominance rather than B cell dysfunction.

Engagement of sialylated glycans with Siglec receptors on myeloid suppressor cells inhibit anti-cancer immunity via CCL2

This study shows that sialylated glycans on myeloid suppressor cells interact with Siglec receptors to suppress anti-cancer immunity through CCL2-mediated mechanisms.

Characterization of CAR T Cells Manufactured using Genetically Engineered Artificial Antigen Presenting Cells

This study compares CAR T cell expansion using genetically engineered aAPCs and antibody-based methods, finding similar potency but different immunophenotypes.

Androgen exposure impairs neutrophil maturation and function within the infected kidney

This study shows that androgen exposure impairs neutrophil maturation and function in the infected kidney, leading to severe UTIs and abscesses in male mice.

Immunogenicity of COVID-19 vaccines and their effect on the HIV reservoir in older people with HIV

This study evaluates the immunogenicity of COVID-19 vaccines in older people with HIV and their impact on HIV reservoirs, finding that three doses are needed for optimal neutralizing responses but vaccines may increase HIV reservoirs in those with persistent viremia.

Hyperglycosylation of prosaposin in tumor DCs promotes immune escape in cancer

This study shows that hyperglycosylation of prosaposin in tumor DCs leads to immune escape by impairing antigen presentation and T cell activation.

Fluorochrome-dependent specific changes in spectral profiles using different compensation beads or human cells in full spectrum cytometry

This study evaluates compensation beads and human/murine cells as reference controls for full spectrum cytometry, showing fluorochrome-specific spectral profile differences.

Spontaneous human CD8 T cell and EAE-inducible human CD4/CD8 T cell lesions in the brain and spinal cord of HLA-DRB1*15-positive multiple sclerosis PBMC humanized mice

This study develops a humanized mouse model of multiple sclerosis using PBMCs from HLA-DRB1*15-positive patients, showing spontaneous and EAE-induced T cell lesions in CNS tissues.

Long-term cytotoxic NK cells with broad anti-tumour capacity proliferate selectively, without exhaustion, after BCG priming and extremely low doses of cytokines

This paper describes a method to generate long-lived, cytotoxic NK cells with broad anti-tumour activity using BCG priming and low-dose cytokines, without cell sorting or exhaustion.

Cross-reactivity of antibodies against T cell markers in the Bank vole (Myodes glareolus)

The paper tests monoclonal antibody cross-reactivity against T cell markers in bank voles and provides sequence analysis of relevant molecules in Cricetidae rodents.

Differential cellular immune responses against Orientia tsutsugamushi Karp and Gilliam strains following acute infection in mice

This study compares immune responses in mice infected with two Orientia tsutsugamushi strains, showing differential cellular activation and cytokine profiles linked to disease severity.

DanioCTC: Injection of circulating tumor cells from metastatic breast cancer patients in zebrafish xenografts for analysis of metastasis

DanioCTC injects patient-derived CTCs into zebrafish embryos to study metastasis, showing distinct dissemination patterns compared to cell line controls.

Post-acute immunological and behavioral sequelae in mice after Omicron infection

This study investigates post-acute immunological and behavioral effects in mice after Omicron infection, showing vaccination reduces pulmonary immune perturbations and behavioral deficits.

Sustained store-operated calcium entry utilizing activated chromatin state leads to instability in iTregs

This study shows that sustained store-operated calcium entry (SOCE) and chromatin accessibility in iTregs lead to phenotypical instability, unlike tTregs.

A combined computational and experimental investigation of the filtration function of splenic macrophages in sickle cell disease

This study combines computational modeling and experiments to show that hypoxia and RBC aggregation enhance macrophage retention of sickle RBCs, contributing to splenic congestion in SCD.

Dichotomy of neutralizing antibody, B cell and T cell responses to SARS-CoV-2 vaccination and protection in healthy adults

The paper identifies four patterns of SARS-CoV-2 vaccine responses, including broad and narrow responders, and links broad responder traits to longer infection-free time after vaccination.

High Frequencies of Antiviral Effector Memory TEM Cells and Memory B Cells Mobilized into Herpes Infected Vaginal Mucosa Associated With Protection Against Genital Herpes

This study shows that CCL28 mobilizes HSV-specific memory B and T cells in vaginal mucosa, providing protection against genital herpes.

Autoreactive B cells in rheumatoid arthritis consist of activated CXCR3+ memory B cells and plasmablasts

The paper identifies CXCR3+ memory B cells and plasmablasts as autoreactive B cells in rheumatoid arthritis, showing cross-reactivity against PTM antigens and high CXCR3 expression.

Disrupted memory T cell expansion in HIV-exposed uninfected infants is preceded by premature skewing of T cell receptor clonality.

This study shows that HIV/ARV-exposed uninfected infants have reduced T cell receptor diversity and altered immune development, increasing infection risk.

Altered glucose utilization and disrupted mitochondrial homeostasis in CD4+ T cells from HIV-positive women on combination anti-retroviral therapy

This study examines metabolic changes in immune cells of HIV-positive women on antiretroviral therapy, finding altered glucose uptake and increased mitochondrial activity in CD4+ T cells.

Longitudinal analysis of memory T follicular helper cells and antibody response following CoronaVac vaccination

This study tracks memory T follicular helper cells and antibody response after three doses of CoronaVac vaccine over 300 days, showing increased neutralizing antibodies and cTFH cells, with correlation between cTFH and antibody titers.

MSCProfiler: An image processing workflow to investigate Mesenchymal Stem Cell heterogeneity using imaging flow cytometry data.

Quantitative flow cytometric selection of tau conformational nanobodies specific for pathological aggregates

This study selects conformational nanobodies specific for aggregated tau using flow cytometry, showing their utility in detecting tau aggregates in mouse and human samples.

Mild/Asymptomatic Maternal SARS-CoV-2 Infection Leads to Immune Paralysis in Fetal Circulation and Immune Dysregulation in Fetal-Placental Tissues

This study examines the effects of mild maternal SARS-CoV-2 infection on fetal immune responses, finding altered inflammatory mediators and immune cell activation in cord blood and placental tissues.

Maintained imbalance of triglycerides, apolipoproteins, energy metabolites and cytokines in long-term COVID-19 syndrome (LTCS) patients

This study identifies persistent metabolic, proteomic, and immunologic imbalances in long-term COVID-19 syndrome (LTCS) patients compared to healthy controls and acute COVID-19 patients.