Pluripotent Stem Cells Research

This study identifies signalling pathways that guide the generation of posterior ventral spinal cord motor neurons from human pluripotent stem cells.

This study shows that co-differentiating human and mouse pluripotent stem cells accelerates human neural differentiation, with gene expression changes correlating with in utero development.

The paper develops cerebral organoids from iPSCs derived from PBMCs to study mitochondrial health across stages and shows preserved mitochondrial function and treatment responses.

The paper uses iPSC-derived neurons from Christianson syndrome patients to show allele-specific responses to rescue strategies, highlighting the need for personalized therapeutic approaches.

This study shows that low Sox2 expression in naive pluripotent stem cells enhances their developmental plasticity without impairing neuroectoderm differentiation.

The paper introduces a GFE assay to measure gastruloid formation efficiency, showing that it discriminates between naive and primed pluripotency states in mouse ESCs.

This study identifies OCT4's role in maintaining pluripotency through metabolic regulation and Stat3 signaling in mouse embryos.

The paper introduces a computational tool to identify key transcription factors in lineage specification and validates PU.1 as a master regulator for generating LSEC-like cells from pluripotent stem cells.

The paper derives two distinct trophectoderm stem cells from human pluripotent stem cells, showing differences in markers, culture requirements, and transcriptome profiles.

The paper evaluates TH-Cre knock-in cell lines for identifying and isolating stem cell-derived dopaminergic neurons in vitro and in vivo.

This study identifies a novel polymer substrate for xeno-free, long-term expansion of human pluripotent stem cells in defined conditions.

This study generates AD patient-derived 3D brain organoids with PSEN2 mutation, showing increased Aβ42/Aβ40 ratio, asynchronous calcium transients, and neuronal hyperactivity.

The paper describes a method to generate trunk-like structures from mouse embryonic stem cells that mimic embryonic development features.

The paper identifies and characterizes formative stem cells in mice and humans, showing they have unique transcriptional and epigenetic features compared to naive and primed pluripotent cells.

This study profiles transcriptional and morphogenetic features of human gastruloids, identifying seven cell types and conserved sorting behaviors during embryogenesis.

This paper demonstrates that mouse embryonic stem cells can generate heart-like structures with in vivo-like morphogenesis, supporting cardiovascular progenitors and early heart field compartments.

This paper reports the derivation of airway basal stem cells from human iPSCs, demonstrating their ability to self-renew and differentiate into airway epithelial cell types, and model lung diseases.

The paper identifies essential enhancers for pluripotency in mouse ES cells using a CRISPR-Cas9 screen and characterizes their target genes and regulatory networks.

This study shows nicotinamide reduces neural progenitor proliferation and enhances catecholaminergic neuron differentiation from mouse embryonic stem cells.

This study uses scRNA-Seq and scATAC-Seq to show that human reprogramming occurs through asynchronous, diverse trajectories with distinct regulatory networks.

This study identifies a miR-290-PFN2 axis that regulates endocytosis and ERK signaling, affecting ESC self-renewal and differentiation.

This study enhances the co-differentiation of intestinal organoids with endothelial cells, showing they share an intestinal-specific transcriptional signature with native cells.

The paper uses single-cell RNA-sequencing to identify multiple neuronal cell types in human midbrain organoids, including dopaminergic, GABAergic, and glutamatergic neurons.

This study develops a reproducible method to differentiate iPSCs into RGCs with high purity using dual SMAD and Wnt pathway inhibition.

This study uses iPSCs from autistic individuals to identify early neurodevelopmental differences during cortical and midbrain differentiation.

This study compares gene expression in human and mouse Purkinje cells using transcriptomics, identifying shared and distinct genetic pathways and a novel human marker gene.

This study compares NuRD's role in human and mouse pluripotent stem cells, finding that NuRD is required for transcriptional responses to differentiation signals in both, but with distinct functional differences between species.

This study shows heat shock reduces Oct4 levels in mouse blastocysts and stem cells via the ubiquitin-proteasome pathway involving Dapk1 and Pin1.

This study investigates the role of PUM1 and PUM2 in human ESCs, showing their involvement in maintaining pluripotency and enhancing cardiomyogenic differentiation.